TY - JOUR
T1 - Influence of prostaglandin F2α analogues on the secretory function of bovine luteal cells and ovarian arterial contractility in vitro
AU - Korzekwa, A. J.
AU - Lukasik, K.
AU - Pilawski, W.
AU - Piotrowska-Tomala, K. K.
AU - Jaroszewski, J. J.
AU - Yoshioka, S.
AU - Okuda, K.
AU - Skarzynski, D. J.
N1 - Funding Information:
This research was supported by the Special Statutory Fund of the Scientific Net of the Polish Ministry of Science and Higher Education (20/E183/SN0013/2008), the Japanese Society for the Promotion of Science (JSPS; 14360168), and the Japanese-Polish Joint Research Project agreement between the JSPS and Polish Academy of Sciences. The authors wish to thank Dr. Stanislaw Okrasa of the Warmia and Mazury University in Olsztyn, Poland, for the P4 antiserum. We also thank the Dainippon Pharmaceutical Co., Ltd., Osaka, Japan for providing recombinant human TNF-α (HF-13), and Dr. Yuichi Yokomizo of the National Institute of Animal Health for providing bovine IFN-γ. The authors are grateful to Mr. H. Jablonski of the experimental farm of the Polish Academy of Sciences in Baranowo, and to Mr. M. Baurycza from the animal farm in Cieszymowo, for their assistance and cooperation in providing animals for the study. We thank Dr. M.M. Bah and Dr. P. Warmowski for technical assistance and animal preparation, and VETMEDICAL (Warsaw, Poland) for the use of the Doppler diagnostic ultrasound scanner.
PY - 2014/1
Y1 - 2014/1
N2 - Although prostaglandin (PG) F2α analogues are routinely used for oestrus synchronisation in cattle, their effects on the function of the bovine corpus luteum (CL), and on ovarian arterial contractility, may not reflect the physiological effects of endogenous PGF2α. In the first of two related experiments, the effects of different analogues of PGF2α (aPGF2α) on the secretory function and apoptosis of cultured bovine cells of the CL were assessed. Enzymatically-isolated bovine luteal cells (from between days 8 and 12 of the oestrous cycle), were stimulated for 24h with naturally-occurring PGF2α or aPGF2α (dinoprost, cloprostenol or luprostiol). Secretion of progesterone (P4) was determined and cellular [Ca2+]i mobilisation, as well as cell viability and apoptosis were measured.Naturally-occurring PGF2α and dinoprost stimulated P4 secretion (P<0.05), whereas cloprostenol and luprostiol did not influence P4 synthesis. The greatest cytotoxic and pro-apoptotic effects were observed in the luprostiol-treated cells, at 37.3% and 202%, respectively (P<0.001). The greatest effect on [Ca2+]i mobilisation in luteal cells was observed post-luprostiol treatment (200%; P<0.001).In a second experiment, the influence of naturally-occurring PGF2α and aPGF2α on ovarian arterial contraction in vitro, were examined. No differences in the effects of dinoprost or naturally-occurring PGF2α were found across the studied parameters. The effects of cloprostenol and luprostiol on luteal cell death, in addition to their effects on ovarian arterial contractility, were much greater than those produced by treatment with naturally-occurring PGF2α.
AB - Although prostaglandin (PG) F2α analogues are routinely used for oestrus synchronisation in cattle, their effects on the function of the bovine corpus luteum (CL), and on ovarian arterial contractility, may not reflect the physiological effects of endogenous PGF2α. In the first of two related experiments, the effects of different analogues of PGF2α (aPGF2α) on the secretory function and apoptosis of cultured bovine cells of the CL were assessed. Enzymatically-isolated bovine luteal cells (from between days 8 and 12 of the oestrous cycle), were stimulated for 24h with naturally-occurring PGF2α or aPGF2α (dinoprost, cloprostenol or luprostiol). Secretion of progesterone (P4) was determined and cellular [Ca2+]i mobilisation, as well as cell viability and apoptosis were measured.Naturally-occurring PGF2α and dinoprost stimulated P4 secretion (P<0.05), whereas cloprostenol and luprostiol did not influence P4 synthesis. The greatest cytotoxic and pro-apoptotic effects were observed in the luprostiol-treated cells, at 37.3% and 202%, respectively (P<0.001). The greatest effect on [Ca2+]i mobilisation in luteal cells was observed post-luprostiol treatment (200%; P<0.001).In a second experiment, the influence of naturally-occurring PGF2α and aPGF2α on ovarian arterial contraction in vitro, were examined. No differences in the effects of dinoprost or naturally-occurring PGF2α were found across the studied parameters. The effects of cloprostenol and luprostiol on luteal cell death, in addition to their effects on ovarian arterial contractility, were much greater than those produced by treatment with naturally-occurring PGF2α.
KW - Arterial contraction
KW - Cattle
KW - Corpus luteum
KW - Luteolysis
KW - PGF
KW - Prostaglandin F
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U2 - 10.1016/j.tvjl.2013.09.021
DO - 10.1016/j.tvjl.2013.09.021
M3 - Article
C2 - 24268486
AN - SCOPUS:84893020241
VL - 199
SP - 131
EP - 137
JO - Veterinary Journal
JF - Veterinary Journal
SN - 1090-0233
IS - 1
ER -