Influence of prostaglandin F2α analogues on the secretory function of bovine luteal cells and ovarian arterial contractility in vitro

A. J. Korzekwa, K. Lukasik, W. Pilawski, K. K. Piotrowska-Tomala, J. J. Jaroszewski, S. Yoshioka, K. Okuda, D. J. Skarzynski

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Although prostaglandin (PG) F analogues are routinely used for oestrus synchronisation in cattle, their effects on the function of the bovine corpus luteum (CL), and on ovarian arterial contractility, may not reflect the physiological effects of endogenous PGF. In the first of two related experiments, the effects of different analogues of PGF (aPGF) on the secretory function and apoptosis of cultured bovine cells of the CL were assessed. Enzymatically-isolated bovine luteal cells (from between days 8 and 12 of the oestrous cycle), were stimulated for 24h with naturally-occurring PGF or aPGF (dinoprost, cloprostenol or luprostiol). Secretion of progesterone (P4) was determined and cellular [Ca2+]i mobilisation, as well as cell viability and apoptosis were measured.Naturally-occurring PGF and dinoprost stimulated P4 secretion (P2+]i mobilisation in luteal cells was observed post-luprostiol treatment (200%; P2α and aPGF on ovarian arterial contraction in vitro, were examined. No differences in the effects of dinoprost or naturally-occurring PGF were found across the studied parameters. The effects of cloprostenol and luprostiol on luteal cell death, in addition to their effects on ovarian arterial contractility, were much greater than those produced by treatment with naturally-occurring PGF.

Original languageEnglish
Pages (from-to)131-137
Number of pages7
JournalVeterinary Journal
Volume199
Issue number1
DOIs
Publication statusPublished - Jan 2014

Fingerprint

Synthetic Prostaglandins
Luteal Cells
luteal cells
Dinoprost
prostaglandins
cloprostenol
cattle
corpus luteum
apoptosis
secretion
estrus synchronization
Cloprostenol
estrous cycle
cell viability
cell death
progesterone
Corpus Luteum
calcium
In Vitro Techniques
Estrus Synchronization

Keywords

  • Arterial contraction
  • Cattle
  • Corpus luteum
  • Luteolysis
  • PGF
  • Prostaglandin F

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Medicine(all)

Cite this

Korzekwa, A. J., Lukasik, K., Pilawski, W., Piotrowska-Tomala, K. K., Jaroszewski, J. J., Yoshioka, S., ... Skarzynski, D. J. (2014). Influence of prostaglandin F2α analogues on the secretory function of bovine luteal cells and ovarian arterial contractility in vitro. Veterinary Journal, 199(1), 131-137. https://doi.org/10.1016/j.tvjl.2013.09.021

Influence of prostaglandin F2α analogues on the secretory function of bovine luteal cells and ovarian arterial contractility in vitro. / Korzekwa, A. J.; Lukasik, K.; Pilawski, W.; Piotrowska-Tomala, K. K.; Jaroszewski, J. J.; Yoshioka, S.; Okuda, K.; Skarzynski, D. J.

In: Veterinary Journal, Vol. 199, No. 1, 01.2014, p. 131-137.

Research output: Contribution to journalArticle

Korzekwa, AJ, Lukasik, K, Pilawski, W, Piotrowska-Tomala, KK, Jaroszewski, JJ, Yoshioka, S, Okuda, K & Skarzynski, DJ 2014, 'Influence of prostaglandin F2α analogues on the secretory function of bovine luteal cells and ovarian arterial contractility in vitro', Veterinary Journal, vol. 199, no. 1, pp. 131-137. https://doi.org/10.1016/j.tvjl.2013.09.021
Korzekwa, A. J. ; Lukasik, K. ; Pilawski, W. ; Piotrowska-Tomala, K. K. ; Jaroszewski, J. J. ; Yoshioka, S. ; Okuda, K. ; Skarzynski, D. J. / Influence of prostaglandin F2α analogues on the secretory function of bovine luteal cells and ovarian arterial contractility in vitro. In: Veterinary Journal. 2014 ; Vol. 199, No. 1. pp. 131-137.
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