Influence of nitric oxide and noradrenaline on prostaglandin F(2α)-induced oxytocin secretion and intracellular calcium mobilization in cultured bovine luteal cells

D. J. Skarzynski, S. Kobayashi, K. Okuda

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Although prostaglandin (PG) F(2α) released from the uterus has been shown to cause regression of the bovine corpus luteum (CL), the neuroendocrine, paracrine, and autocrine mechanisms regulating luteolysis and PGF(2α) action in the CL are not fully understood. A number of substances produced locally in the CL may be involved in maintaning the equilibrium between luteal development and its regression. The present study was carried out to determine whether noradrenaline (NA) and nitric oxide (NO) regulate the sensitivity of the bovine CL to PGF(2α) in vitro and modulate a positive feedback cascade between PGF(2α) and luteal oxytocin (OT) in cows. Bovine luteal cells (Days 8-12 of the estrous cycle) cultured in glass tubes were pre-exposed to NA (10-5 M) or an NO donor (S-nitroso-N-acetylpenicillamine [S-NAP]; 10-4 M) before stimulation with PGF(2α) (10-6 M). Noradrenaline significantly stimulated the release of progesterone (P4), OT, PGF(2α), and PGE2 (P < 0.01); however, S-NAP inhibited P4 and OT secretion (P < 0.05). Oxytocin secretion and the intracellular level of free Ca2+ ([Ca2+](i)) were measured as indicators of CL sensitivity to PGF(2α). Prostaglandin F(2α) increased both the amount of OT secretion and [Ca2+](i) by approximately two times the amount before (both P < 0.05). The S-NAP amplified the effect of PGF(2α) on [Ca2+](i) and OT secretion (both P < 0.001), whereas NA diminished the stimulatory effects of PGF(2α) on [Ca2+](i) (P < 0.05). Moreover, PGF(2α) did not exert any additionally effects on OT secretion in NA-pretreated cells. The overall results suggest that adrenergic and nitrergic agents play opposite roles in the regulation of bovine CL function. While NA stimulates P4 and OT secretion, NO may inhibit it in bovine CL. Both NA and NO are likely to stimulate the synthesis of luteal PGs and to modulate the action of PGF(2α). Noradrenaline may be the factor that is responsible for the limited action of PGF(2α) on CL and may be involved in the protection of the CL against premature luteolysis. In contrast, NO augments PGF(2α) action on CL and it may be involved in the course of luteolysis.

Original languageEnglish
Pages (from-to)1000-1005
Number of pages6
JournalBiology of reproduction
Volume63
Issue number4
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • Calcium
  • Catecholamines
  • Corpus luteum
  • Corpus luteum function
  • Signal transduction

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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