TY - JOUR
T1 - Influence of methylprednisolone on the intracellular antimicrobial activity of erythromycin and clindamycin against legionella pneumophila
AU - Higa, Futoshi
AU - Saito, Atsushi
AU - Inadome, Jun
AU - Kusano, Nobuchika
AU - Kitsukawa, Keizo
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 1993/6
Y1 - 1993/6
N2 - We have investigated the effect of methylprednisolone on the intracellular activity of erythromycin and clindamycin in vitro. An assay system was developed for the determination of intracellular activity of antibiotics against Legionella pneumophila using Guinea pig resident alveolar macrophages. Erythromycin at a concentration of 0·625 mg/L (5 × MIC) and clindamycin at a concentration of 8 mg/L (MIC) inhibited the growth of a single strain of L. pneumophila in macrophages, whilst ceftizoxime at a concentration of 0·625 mg/L (5 × MIC) did not. Methylprednisolone at therapeutic concentrations did not affect the intracellular antibacterial activity of either erythromycin or clindamycin against L. pneumophila. We found no direct effect of methylprednisolone on the intracellular antibacterial activity of either erythomycin or cindamycin.
AB - We have investigated the effect of methylprednisolone on the intracellular activity of erythromycin and clindamycin in vitro. An assay system was developed for the determination of intracellular activity of antibiotics against Legionella pneumophila using Guinea pig resident alveolar macrophages. Erythromycin at a concentration of 0·625 mg/L (5 × MIC) and clindamycin at a concentration of 8 mg/L (MIC) inhibited the growth of a single strain of L. pneumophila in macrophages, whilst ceftizoxime at a concentration of 0·625 mg/L (5 × MIC) did not. Methylprednisolone at therapeutic concentrations did not affect the intracellular antibacterial activity of either erythromycin or clindamycin against L. pneumophila. We found no direct effect of methylprednisolone on the intracellular antibacterial activity of either erythomycin or cindamycin.
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U2 - 10.1093/jac/31.6.901
DO - 10.1093/jac/31.6.901
M3 - Article
C2 - 8360127
AN - SCOPUS:0027313670
VL - 31
SP - 901
EP - 908
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
SN - 0305-7453
IS - 6
ER -