Influence of methylprednisolone on the intracellular antimicrobial activity of erythromycin and clindamycin against legionella pneumophila

Futoshi Higa; Atsushi Saito; Jun Inadome; Nobuchika Kusano; Keizo Kitsukawa

doi:10.1093/jac/31.6.901

Influence of methylprednisolone on the intracellular antimicrobial activity of erythromycin and clindamycin against legionella pneumophila

Futoshi Higa, Atsushi Saito, Jun Inadome, Nobuchika Kusano, Keizo Kitsukawa

Research output: Contribution to journal › Article

2 Citations (Scopus)

Abstract

We have investigated the effect of methylprednisolone on the intracellular activity of erythromycin and clindamycin in vitro. An assay system was developed for the determination of intracellular activity of antibiotics against Legionella pneumophila using Guinea pig resident alveolar macrophages. Erythromycin at a concentration of 0·625 mg/L (5 × MIC) and clindamycin at a concentration of 8 mg/L (MIC) inhibited the growth of a single strain of L. pneumophila in macrophages, whilst ceftizoxime at a concentration of 0·625 mg/L (5 × MIC) did not. Methylprednisolone at therapeutic concentrations did not affect the intracellular antibacterial activity of either erythromycin or clindamycin against L. pneumophila. We found no direct effect of methylprednisolone on the intracellular antibacterial activity of either erythomycin or cindamycin.

Original language	English
Pages (from-to)	901-908
Number of pages	8
Journal	Journal of Antimicrobial Chemotherapy
Volume	31
Issue number	6
DOIs	https://doi.org/10.1093/jac/31.6.901
Publication status	Published - Jun 1 1993
Externally published	Yes

ASJC Scopus subject areas

Pharmacology
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

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Higa, F., Saito, A., Inadome, J., Kusano, N., & Kitsukawa, K. (1993). Influence of methylprednisolone on the intracellular antimicrobial activity of erythromycin and clindamycin against legionella pneumophila. Journal of Antimicrobial Chemotherapy, 31(6), 901-908. https://doi.org/10.1093/jac/31.6.901

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abstract = "We have investigated the effect of methylprednisolone on the intracellular activity of erythromycin and clindamycin in vitro. An assay system was developed for the determination of intracellular activity of antibiotics against Legionella pneumophila using Guinea pig resident alveolar macrophages. Erythromycin at a concentration of 0·625 mg/L (5 × MIC) and clindamycin at a concentration of 8 mg/L (MIC) inhibited the growth of a single strain of L. pneumophila in macrophages, whilst ceftizoxime at a concentration of 0·625 mg/L (5 × MIC) did not. Methylprednisolone at therapeutic concentrations did not affect the intracellular antibacterial activity of either erythromycin or clindamycin against L. pneumophila. We found no direct effect of methylprednisolone on the intracellular antibacterial activity of either erythomycin or cindamycin.",

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AU - Kitsukawa, Keizo

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