Influence of intraalveolar oxygen concentration on lung preservation in a rabbit model

W. Weder, B. Harper, S. Shimokawa, S. Miyoshi, H. Date, H. Schreinemakers, T. Egan, J. D. Cooper

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We previously reported the use of an inexpensive screening model for lung preservation involving ventilation and perfusion of excised rabbit lungs after their preservation. We now have extended this model by perfusing the preserved lung in cross circulation with an anesthetized rabbit to permit stable reperfusion of the preserved lungs for 60 minutes. With this model we compared the results of lung preservation with the lungs inflated with nitrogen, room air, or 100% oxygen during 24 hours of hypthermic storage. Four groups of animals were studied: group 1, excision and immediate evaluation; group 2, inflation with room air and storage for 24 hours at 10° C; group 3, same as group 2, with 100% oxygen for inflation during storage; group 4, same as group 2, with 100% nitrogen for inflation during storage. Assessment of the ex vivo perfused lung consisted of (1) blood gas analysis of inflow and outflow blood at 10-minute intervals; (2) continuous pulmonary artery and airway pressure monitoring; (3) measurement of pulmonary venous oxygen tension after 1 hour of reperfusion, with inflow oxygen tension adjusted to 15 mm Hg; (4) wet/dry weight ratio. We conclude that the paracorporeal circuit does not, in itself, cause lung injury over a 1-hour period; lungs preserved with nitrogen inflation rapidly became edematous and failed to function on reperfusion. Preservation with 100% oxygen inflation appears superior to inflation with room air.

Original languageEnglish
Pages (from-to)1037-1043
Number of pages7
JournalJournal of Thoracic and Cardiovascular Surgery
Volume101
Issue number6
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Economic Inflation
Oxygen
Rabbits
Lung
Reperfusion
Nitrogen
Air
Cross Circulation
Blood Gas Analysis
Lung Injury
Pulmonary Artery
Ventilation
Perfusion
Pressure
Weights and Measures

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Weder, W., Harper, B., Shimokawa, S., Miyoshi, S., Date, H., Schreinemakers, H., ... Cooper, J. D. (1991). Influence of intraalveolar oxygen concentration on lung preservation in a rabbit model. Journal of Thoracic and Cardiovascular Surgery, 101(6), 1037-1043.

Influence of intraalveolar oxygen concentration on lung preservation in a rabbit model. / Weder, W.; Harper, B.; Shimokawa, S.; Miyoshi, S.; Date, H.; Schreinemakers, H.; Egan, T.; Cooper, J. D.

In: Journal of Thoracic and Cardiovascular Surgery, Vol. 101, No. 6, 1991, p. 1037-1043.

Research output: Contribution to journalArticle

Weder, W, Harper, B, Shimokawa, S, Miyoshi, S, Date, H, Schreinemakers, H, Egan, T & Cooper, JD 1991, 'Influence of intraalveolar oxygen concentration on lung preservation in a rabbit model', Journal of Thoracic and Cardiovascular Surgery, vol. 101, no. 6, pp. 1037-1043.
Weder W, Harper B, Shimokawa S, Miyoshi S, Date H, Schreinemakers H et al. Influence of intraalveolar oxygen concentration on lung preservation in a rabbit model. Journal of Thoracic and Cardiovascular Surgery. 1991;101(6):1037-1043.
Weder, W. ; Harper, B. ; Shimokawa, S. ; Miyoshi, S. ; Date, H. ; Schreinemakers, H. ; Egan, T. ; Cooper, J. D. / Influence of intraalveolar oxygen concentration on lung preservation in a rabbit model. In: Journal of Thoracic and Cardiovascular Surgery. 1991 ; Vol. 101, No. 6. pp. 1037-1043.
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