Influence of androgen on tyrosine hydroxylase mRNA in adrenal medulla of spontaneously hypertensive rats

T. Kumai, M. Tanaka, M. Watanabe, Hironori Nakura, S. Kobayashi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We investigated the effects of castration and testosterone propionate on tyrosine hydroxylase mRNA, its activity, and catecholamine synthesis in the adrenal medulla of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Four-week-old male rats were castrated. Testosterone propionate (500 μg per rat) was administered subcutaneously twice a week to castrated rats (between 14 and 25 weeks of age). Systolic pressure was measured at the age of 25 weeks, and rats were decapitated. The systolic pressure of castrated SHR was significantly lower than that of control and testosterone- replaced SHR. Epinephrine and norepinephrine levels, tyrosine hydroxylase activity, and tyrosine hydroxylase mRNA in the adrenal medulla of castrated SHR were significantly lower than in control and testosterone-replaced SHR. Systolic pressure and epinephrine and norepinephrine levels, tyrosine hydroxylase activity, and tyrosine hydroxylase mRNA levels in the adrenal medulla of WKY showed no significant differences among the control, castrated, and testosterone-replaced groups. These results suggest that androgens contribute to the development and maintenance of hypertension in SHR via sustained enhancement of tyrosine hydroxylase synthesis in the adrenal medulla, leading to increased epinephrine and norepinephrine levels.

Original languageEnglish
Pages (from-to)208-212
Number of pages5
JournalHypertension
Volume26
Issue number1
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Adrenal Medulla
Tyrosine 3-Monooxygenase
Inbred SHR Rats
Androgens
Messenger RNA
Epinephrine
Testosterone
Testosterone Propionate
Norepinephrine
Inbred WKY Rats
Blood Pressure
Castration
Catecholamines
Maintenance
Hypertension

Keywords

  • adrenal medulla
  • androgens
  • epinephrine
  • norepinephrine
  • rats, inbred SHR
  • RNA, messenger
  • tyrosine 3-monooxygenase

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Influence of androgen on tyrosine hydroxylase mRNA in adrenal medulla of spontaneously hypertensive rats. / Kumai, T.; Tanaka, M.; Watanabe, M.; Nakura, Hironori; Kobayashi, S.

In: Hypertension, Vol. 26, No. 1, 1995, p. 208-212.

Research output: Contribution to journalArticle

Kumai, T, Tanaka, M, Watanabe, M, Nakura, H & Kobayashi, S 1995, 'Influence of androgen on tyrosine hydroxylase mRNA in adrenal medulla of spontaneously hypertensive rats', Hypertension, vol. 26, no. 1, pp. 208-212.
Kumai, T. ; Tanaka, M. ; Watanabe, M. ; Nakura, Hironori ; Kobayashi, S. / Influence of androgen on tyrosine hydroxylase mRNA in adrenal medulla of spontaneously hypertensive rats. In: Hypertension. 1995 ; Vol. 26, No. 1. pp. 208-212.
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