Abstract
We investigated the effects of castration and testosterone propionate on tyrosine hydroxylase mRNA, its activity, and catecholamine synthesis in the adrenal medulla of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Four-week-old male rats were castrated. Testosterone propionate (500 μg per rat) was administered subcutaneously twice a week to castrated rats (between 14 and 25 weeks of age). Systolic pressure was measured at the age of 25 weeks, and rats were decapitated. The systolic pressure of castrated SHR was significantly lower than that of control and testosterone- replaced SHR. Epinephrine and norepinephrine levels, tyrosine hydroxylase activity, and tyrosine hydroxylase mRNA in the adrenal medulla of castrated SHR were significantly lower than in control and testosterone-replaced SHR. Systolic pressure and epinephrine and norepinephrine levels, tyrosine hydroxylase activity, and tyrosine hydroxylase mRNA levels in the adrenal medulla of WKY showed no significant differences among the control, castrated, and testosterone-replaced groups. These results suggest that androgens contribute to the development and maintenance of hypertension in SHR via sustained enhancement of tyrosine hydroxylase synthesis in the adrenal medulla, leading to increased epinephrine and norepinephrine levels.
| Original language | English |
|---|---|
| Pages (from-to) | 208-212 |
| Number of pages | 5 |
| Journal | Hypertension |
| Volume | 26 |
| Issue number | 1 |
| Publication status | Published - 1995 |
| Externally published | Yes |
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Keywords
- adrenal medulla
- androgens
- epinephrine
- norepinephrine
- rats, inbred SHR
- RNA, messenger
- tyrosine 3-monooxygenase
ASJC Scopus subject areas
- Internal Medicine
Cite this
Influence of androgen on tyrosine hydroxylase mRNA in adrenal medulla of spontaneously hypertensive rats. / Kumai, T.; Tanaka, M.; Watanabe, M.; Nakura, Hironori; Kobayashi, S.
In: Hypertension, Vol. 26, No. 1, 1995, p. 208-212.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of androgen on tyrosine hydroxylase mRNA in adrenal medulla of spontaneously hypertensive rats
AU - Kumai, T.
AU - Tanaka, M.
AU - Watanabe, M.
AU - Nakura, Hironori
AU - Kobayashi, S.
PY - 1995
Y1 - 1995
N2 - We investigated the effects of castration and testosterone propionate on tyrosine hydroxylase mRNA, its activity, and catecholamine synthesis in the adrenal medulla of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Four-week-old male rats were castrated. Testosterone propionate (500 μg per rat) was administered subcutaneously twice a week to castrated rats (between 14 and 25 weeks of age). Systolic pressure was measured at the age of 25 weeks, and rats were decapitated. The systolic pressure of castrated SHR was significantly lower than that of control and testosterone- replaced SHR. Epinephrine and norepinephrine levels, tyrosine hydroxylase activity, and tyrosine hydroxylase mRNA in the adrenal medulla of castrated SHR were significantly lower than in control and testosterone-replaced SHR. Systolic pressure and epinephrine and norepinephrine levels, tyrosine hydroxylase activity, and tyrosine hydroxylase mRNA levels in the adrenal medulla of WKY showed no significant differences among the control, castrated, and testosterone-replaced groups. These results suggest that androgens contribute to the development and maintenance of hypertension in SHR via sustained enhancement of tyrosine hydroxylase synthesis in the adrenal medulla, leading to increased epinephrine and norepinephrine levels.
AB - We investigated the effects of castration and testosterone propionate on tyrosine hydroxylase mRNA, its activity, and catecholamine synthesis in the adrenal medulla of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Four-week-old male rats were castrated. Testosterone propionate (500 μg per rat) was administered subcutaneously twice a week to castrated rats (between 14 and 25 weeks of age). Systolic pressure was measured at the age of 25 weeks, and rats were decapitated. The systolic pressure of castrated SHR was significantly lower than that of control and testosterone- replaced SHR. Epinephrine and norepinephrine levels, tyrosine hydroxylase activity, and tyrosine hydroxylase mRNA in the adrenal medulla of castrated SHR were significantly lower than in control and testosterone-replaced SHR. Systolic pressure and epinephrine and norepinephrine levels, tyrosine hydroxylase activity, and tyrosine hydroxylase mRNA levels in the adrenal medulla of WKY showed no significant differences among the control, castrated, and testosterone-replaced groups. These results suggest that androgens contribute to the development and maintenance of hypertension in SHR via sustained enhancement of tyrosine hydroxylase synthesis in the adrenal medulla, leading to increased epinephrine and norepinephrine levels.
KW - adrenal medulla
KW - androgens
KW - epinephrine
KW - norepinephrine
KW - rats, inbred SHR
KW - RNA, messenger
KW - tyrosine 3-monooxygenase
UR - http://www.scopus.com/inward/record.url?scp=0029007964&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029007964&partnerID=8YFLogxK
M3 - Article
C2 - 7607726
AN - SCOPUS:0029007964
VL - 26
SP - 208
EP - 212
JO - Hypertension
JF - Hypertension
SN - 0194-911X
IS - 1
ER -