Inflammatory markers in exhaled breath condensate from patients with asthma

Tomoe Ueno, Mikio Kataoka, Atsushi Hirano, Kouji Iio, Yasushi Tanimoto, Arihiko Kanehiro, Chiharu Okada, Ryo Soda, Kiyoshi Takahashi, Mitsune Tanimoto

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


Background and objective: Evaluation of airway inflammation is important for the diagnosis and treatment of asthma. Exhaled breath condensate (EBC) is a minimally invasive method for assessing inflammation and may be useful for monitoring airway inflammation in asthma. The aims of this study were to establish an EBC collection method, to assess biomarkers reflecting asthmatic airway inflammation, and to determine the relationship of these biomarkers with asthma severity and lung function. Methods: Fifty-eight non-smoking healthy subjects, seven asymptomatic smokers, nine subjects with common cold and 55 asthmatics with disease severity ranging from mild intermittent to severe persistent were studied. The efficacy of a pipette method was compared with that of a commercial collecting device. pH, CRP, albumin, hydrogen peroxide (H 2O2) and nitrite/nitrate levels were measured in EBC. Results: Except for the quantity of EBC collected and albumin levels, there were no differences between the commercial method and the pipette method in levels of biomarkers measured. Levels of CRP, H2O2 and nitrite/nitrate were significantly higher in the asthma group than that in the control group. In terms of asthma severity, pH and levels of CRP, H 2O2 and nitrate were significantly higher in the mild persistent group than that in the other groups. In addition, H2O 2 levels in EBC correlated significantly with the level of nitrite/nitrate. FEV1 and PEF showed significant negative correlations with H2O2 and nitrite/nitrate levels. Conclusion: Measurement of EBC biomarkers is a non-invasive and useful way to evaluate airway inflammation in patients with asthma.

Original languageEnglish
Pages (from-to)654-663
Number of pages10
Issue number5
Publication statusPublished - Jul 2008
Externally publishedYes


  • Asthma
  • Biochemistry
  • Clinical allergy and immunology
  • Exhaled breath condensate

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine


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