Inflammatory diarrhea due to enteroaggregative Escherichia coli

Evidence from clinical and mice model studies

Dhira Rani Saha, Sucharita Guin, Rajendran Krishnan, Dhrubajyoti Nag, Hemanta Koley, Sumio Shinoda, Thandavarayan Ramamurthy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: This study was conducted to determine the role of enteroaggregative Escherichia coli (EAEC) in inflammatory diarrhea among hospitalized patients in Kolkata. The inflammatory pathogenesis of EAEC was established in mice model and histopathological studies. Presence of fecal leucocytes (FLCs) can be suspected for EAEC infection solely or as a mixed with other enteric pathogens. Methods. Active surveillance was conducted for 2 years on 2 random days per week with every 5§ssup§th§esup§ patient admitted to the Infectious Diseases Hospital (IDH). Diarrheal samples were processed by conventional culture, microscopy, ELISA and molecular methods. Two EAEC isolated as sole pathogens were examined in mice after induced intestinal infection. The intestinal tissue samples were processed to analyze the histological changes. Results: Of the 2519 samples screened, fecal leucocytes, erythrocytes and occult blood were detected in 1629 samples. Most of the patients had acute watery diarrhea (75%) and vomiting (78%). Vibrio cholerae O1 was the main pathogen in patients of 5-10 years age group (33%). Shigellosis was more in children from 2-5 years of age (19%), whereas children

Original languageEnglish
Article number36
JournalGut Pathogens
Volume5
Issue number1
DOIs
Publication statusPublished - Dec 3 2013
Externally publishedYes

Fingerprint

Diarrhea
Escherichia coli
Leukocytes
Vibrio cholerae O1
Bacillary Dysentery
Escherichia coli Infections
Occult Blood
Vomiting
Communicable Diseases
Microscopy
Age Groups
Erythrocytes
Enzyme-Linked Immunosorbent Assay
Infection

Keywords

  • Diarrhea
  • EAEC
  • Fecal leucocytes
  • Histology
  • Inflammation

ASJC Scopus subject areas

  • Gastroenterology
  • Infectious Diseases
  • Microbiology
  • Parasitology
  • Virology

Cite this

Saha, D. R., Guin, S., Krishnan, R., Nag, D., Koley, H., Shinoda, S., & Ramamurthy, T. (2013). Inflammatory diarrhea due to enteroaggregative Escherichia coli: Evidence from clinical and mice model studies. Gut Pathogens, 5(1), [36]. https://doi.org/10.1186/1757-4749-5-36

Inflammatory diarrhea due to enteroaggregative Escherichia coli : Evidence from clinical and mice model studies. / Saha, Dhira Rani; Guin, Sucharita; Krishnan, Rajendran; Nag, Dhrubajyoti; Koley, Hemanta; Shinoda, Sumio; Ramamurthy, Thandavarayan.

In: Gut Pathogens, Vol. 5, No. 1, 36, 03.12.2013.

Research output: Contribution to journalArticle

Saha, DR, Guin, S, Krishnan, R, Nag, D, Koley, H, Shinoda, S & Ramamurthy, T 2013, 'Inflammatory diarrhea due to enteroaggregative Escherichia coli: Evidence from clinical and mice model studies', Gut Pathogens, vol. 5, no. 1, 36. https://doi.org/10.1186/1757-4749-5-36
Saha, Dhira Rani ; Guin, Sucharita ; Krishnan, Rajendran ; Nag, Dhrubajyoti ; Koley, Hemanta ; Shinoda, Sumio ; Ramamurthy, Thandavarayan. / Inflammatory diarrhea due to enteroaggregative Escherichia coli : Evidence from clinical and mice model studies. In: Gut Pathogens. 2013 ; Vol. 5, No. 1.
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