Inflammatory cytokine-induced expression of vasohibin-1 by rheumatoid synovial fibroblasts

Kohei Miyake, Keiichiro Nishida, Yasutaka Kadota, Hiroko Yamasaki, Tatsuyo Nasu, Daisuke Saitou, Katsuyuki Tanabe, Hikaru Sonoda, Yasufumi Sato, Yohei Maeshima, Hirofumi Makino

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Angiogenesis is an essential event in the development of synovial inflammation in rheumatoid arthritis (RA). The aim of the current study was to investigate the expression of vasohibin-1, a novel endothe-lium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor, in the RA synovium, and to test the effect of inflammatory cytokines on the expression of vasohibin-1 by RA synovial fibroblasts (RASFs). Synovial tissue samples were obtained at surgery from patients with osteoarthritis (OA) and RA, and subjected to immunohistochemistry to investigate the expression and distribution of vasohibin-1 relevant to the degree of synovial inflammation. In an in vitro analysis, RASFs were used to examine the expression of vasohibin-1 and VEGF mRNA by real-time PCR after stimulation with VEGF or inflammatory cytokines under normoxic or hypoxic conditions. The immu-nohistochemical results showed that vasohibin-1 was expressed in synovial lining cells, endothelial cells, and synovial fibroblasts. In synovial tissue, there was a significant correlation between the expression of vasohibin-1 and histological inflammation score (ρ - 0.002, r = 0.842). In wtro, stimulation with VEGF induced the expression of vasohibin-1 mRNA in RASFs under normoxic conditions, and stimulation with cytokines induced vasohibin-1 mRNA expression under a hypoxic condition. These results suggest that vasohibin-1 was expressed in RA synovial tissue and might be regulated by inflammatory cytokines.

Original languageEnglish
Pages (from-to)349-358
Number of pages10
JournalActa Medica Okayama
Volume63
Issue number6
Publication statusPublished - Dec 2009

Fingerprint

Fibroblasts
Vascular Endothelial Growth Factor A
Rheumatoid Arthritis
Cytokines
Tissue
Messenger RNA
Inflammation
Angiogenesis Inhibitors
Endothelial cells
Linings
Synovial Membrane
Surgery
Osteoarthritis
Real-Time Polymerase Chain Reaction
Endothelial Cells
Immunohistochemistry

Keywords

  • Angiogenesis
  • Rheumatoid arthritis
  • Synovial membrane
  • Vasohibin-1
  • VEGF

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Inflammatory cytokine-induced expression of vasohibin-1 by rheumatoid synovial fibroblasts. / Miyake, Kohei; Nishida, Keiichiro; Kadota, Yasutaka; Yamasaki, Hiroko; Nasu, Tatsuyo; Saitou, Daisuke; Tanabe, Katsuyuki; Sonoda, Hikaru; Sato, Yasufumi; Maeshima, Yohei; Makino, Hirofumi.

In: Acta Medica Okayama, Vol. 63, No. 6, 12.2009, p. 349-358.

Research output: Contribution to journalArticle

Miyake, K, Nishida, K, Kadota, Y, Yamasaki, H, Nasu, T, Saitou, D, Tanabe, K, Sonoda, H, Sato, Y, Maeshima, Y & Makino, H 2009, 'Inflammatory cytokine-induced expression of vasohibin-1 by rheumatoid synovial fibroblasts', Acta Medica Okayama, vol. 63, no. 6, pp. 349-358.
Miyake K, Nishida K, Kadota Y, Yamasaki H, Nasu T, Saitou D et al. Inflammatory cytokine-induced expression of vasohibin-1 by rheumatoid synovial fibroblasts. Acta Medica Okayama. 2009 Dec;63(6):349-358.
Miyake, Kohei ; Nishida, Keiichiro ; Kadota, Yasutaka ; Yamasaki, Hiroko ; Nasu, Tatsuyo ; Saitou, Daisuke ; Tanabe, Katsuyuki ; Sonoda, Hikaru ; Sato, Yasufumi ; Maeshima, Yohei ; Makino, Hirofumi. / Inflammatory cytokine-induced expression of vasohibin-1 by rheumatoid synovial fibroblasts. In: Acta Medica Okayama. 2009 ; Vol. 63, No. 6. pp. 349-358.
@article{bd900b6d32d94ad3aca32ddb8561e54d,
title = "Inflammatory cytokine-induced expression of vasohibin-1 by rheumatoid synovial fibroblasts",
abstract = "Angiogenesis is an essential event in the development of synovial inflammation in rheumatoid arthritis (RA). The aim of the current study was to investigate the expression of vasohibin-1, a novel endothe-lium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor, in the RA synovium, and to test the effect of inflammatory cytokines on the expression of vasohibin-1 by RA synovial fibroblasts (RASFs). Synovial tissue samples were obtained at surgery from patients with osteoarthritis (OA) and RA, and subjected to immunohistochemistry to investigate the expression and distribution of vasohibin-1 relevant to the degree of synovial inflammation. In an in vitro analysis, RASFs were used to examine the expression of vasohibin-1 and VEGF mRNA by real-time PCR after stimulation with VEGF or inflammatory cytokines under normoxic or hypoxic conditions. The immu-nohistochemical results showed that vasohibin-1 was expressed in synovial lining cells, endothelial cells, and synovial fibroblasts. In synovial tissue, there was a significant correlation between the expression of vasohibin-1 and histological inflammation score (ρ - 0.002, r = 0.842). In wtro, stimulation with VEGF induced the expression of vasohibin-1 mRNA in RASFs under normoxic conditions, and stimulation with cytokines induced vasohibin-1 mRNA expression under a hypoxic condition. These results suggest that vasohibin-1 was expressed in RA synovial tissue and might be regulated by inflammatory cytokines.",
keywords = "Angiogenesis, Rheumatoid arthritis, Synovial membrane, Vasohibin-1, VEGF",
author = "Kohei Miyake and Keiichiro Nishida and Yasutaka Kadota and Hiroko Yamasaki and Tatsuyo Nasu and Daisuke Saitou and Katsuyuki Tanabe and Hikaru Sonoda and Yasufumi Sato and Yohei Maeshima and Hirofumi Makino",
year = "2009",
month = "12",
language = "English",
volume = "63",
pages = "349--358",
journal = "Acta Medica Okayama",
issn = "0386-300X",
publisher = "Okayama University",
number = "6",

}

TY - JOUR

T1 - Inflammatory cytokine-induced expression of vasohibin-1 by rheumatoid synovial fibroblasts

AU - Miyake, Kohei

AU - Nishida, Keiichiro

AU - Kadota, Yasutaka

AU - Yamasaki, Hiroko

AU - Nasu, Tatsuyo

AU - Saitou, Daisuke

AU - Tanabe, Katsuyuki

AU - Sonoda, Hikaru

AU - Sato, Yasufumi

AU - Maeshima, Yohei

AU - Makino, Hirofumi

PY - 2009/12

Y1 - 2009/12

N2 - Angiogenesis is an essential event in the development of synovial inflammation in rheumatoid arthritis (RA). The aim of the current study was to investigate the expression of vasohibin-1, a novel endothe-lium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor, in the RA synovium, and to test the effect of inflammatory cytokines on the expression of vasohibin-1 by RA synovial fibroblasts (RASFs). Synovial tissue samples were obtained at surgery from patients with osteoarthritis (OA) and RA, and subjected to immunohistochemistry to investigate the expression and distribution of vasohibin-1 relevant to the degree of synovial inflammation. In an in vitro analysis, RASFs were used to examine the expression of vasohibin-1 and VEGF mRNA by real-time PCR after stimulation with VEGF or inflammatory cytokines under normoxic or hypoxic conditions. The immu-nohistochemical results showed that vasohibin-1 was expressed in synovial lining cells, endothelial cells, and synovial fibroblasts. In synovial tissue, there was a significant correlation between the expression of vasohibin-1 and histological inflammation score (ρ - 0.002, r = 0.842). In wtro, stimulation with VEGF induced the expression of vasohibin-1 mRNA in RASFs under normoxic conditions, and stimulation with cytokines induced vasohibin-1 mRNA expression under a hypoxic condition. These results suggest that vasohibin-1 was expressed in RA synovial tissue and might be regulated by inflammatory cytokines.

AB - Angiogenesis is an essential event in the development of synovial inflammation in rheumatoid arthritis (RA). The aim of the current study was to investigate the expression of vasohibin-1, a novel endothe-lium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor, in the RA synovium, and to test the effect of inflammatory cytokines on the expression of vasohibin-1 by RA synovial fibroblasts (RASFs). Synovial tissue samples were obtained at surgery from patients with osteoarthritis (OA) and RA, and subjected to immunohistochemistry to investigate the expression and distribution of vasohibin-1 relevant to the degree of synovial inflammation. In an in vitro analysis, RASFs were used to examine the expression of vasohibin-1 and VEGF mRNA by real-time PCR after stimulation with VEGF or inflammatory cytokines under normoxic or hypoxic conditions. The immu-nohistochemical results showed that vasohibin-1 was expressed in synovial lining cells, endothelial cells, and synovial fibroblasts. In synovial tissue, there was a significant correlation between the expression of vasohibin-1 and histological inflammation score (ρ - 0.002, r = 0.842). In wtro, stimulation with VEGF induced the expression of vasohibin-1 mRNA in RASFs under normoxic conditions, and stimulation with cytokines induced vasohibin-1 mRNA expression under a hypoxic condition. These results suggest that vasohibin-1 was expressed in RA synovial tissue and might be regulated by inflammatory cytokines.

KW - Angiogenesis

KW - Rheumatoid arthritis

KW - Synovial membrane

KW - Vasohibin-1

KW - VEGF

UR - http://www.scopus.com/inward/record.url?scp=74349090158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74349090158&partnerID=8YFLogxK

M3 - Article

C2 - 20035291

AN - SCOPUS:74349090158

VL - 63

SP - 349

EP - 358

JO - Acta Medica Okayama

JF - Acta Medica Okayama

SN - 0386-300X

IS - 6

ER -