Inflammatory airway responses by nasal inoculation of suspended particulate matter in NC/Nga mice

Keiki Ogino, Noriko Takahashi, Masayuki Kubo, Akihito Takeuchi, Motoharu Nakagiri, Yoshihisa Fujikura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To evaluate the allergic effect of airborne particulate matter (PM) on the airway, separated soluble supernatant (Sup) and insoluble precipitate (Pre) in suspended PM were inoculated into NC/Nga mice with a high sensitivity for mite allergens. Sup, Pre, or both Sup and Pre with or without pronase treatment were inoculated via the nasal route five times for sensitization and a challenge inoculation on the 11th day in NC/Nga mice. On the 14th day, mice were examined for airway hyperresponsiveness (AHR), bronchoalveolar lavage fluid (BALF) cell count, mRNA expression of Th1 and Th2 cytokines in the lung tissue, and histopathology. Synergistic effects of Sup and Pre were observed as increases in AHR and a histopathological change of Periodic acid-Schiff (PAS) staining. Increases in neutrophils, macrophages, and lymphocytes of BALF cells were dependent on Pre. The expression of IL-4 mRNA was increased by Sup, and those of IL-5 mRNA and Il-13 mRNA was increased by Sup and Pre. Augmented AHR, mRNA expression of IL-4, peribronchial inflammation, and PAS staining by Sup plus Pre were attenuated by treatment of Sup with pronase to digest proteins. These results suggest that some proteins of ambient PM may be important environmental factors for AHR and airway inflammation with the aid of insoluble particulates, although some soluble factors such as endotoxins cannot be ruled out.

Original languageEnglish
Pages (from-to)642-654
Number of pages13
JournalEnvironmental Toxicology
Volume29
Issue number6
DOIs
Publication statusPublished - 2014

Fingerprint

Particulate Matter
suspended particulate matter
Nose
inoculation
particulate matter
Precipitates
Messenger RNA
protein
histopathology
fluid
acid
Pronase
Periodic Acid
mite
Bronchoalveolar Lavage Fluid
environmental factor
Interleukin-4
Staining and Labeling
Inflammation
Mites

Keywords

  • Airway inflammation
  • NC/Nga mouse
  • Particulate matter

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Management, Monitoring, Policy and Law

Cite this

Ogino, K., Takahashi, N., Kubo, M., Takeuchi, A., Nakagiri, M., & Fujikura, Y. (2014). Inflammatory airway responses by nasal inoculation of suspended particulate matter in NC/Nga mice. Environmental Toxicology, 29(6), 642-654. https://doi.org/10.1002/tox.21791

Inflammatory airway responses by nasal inoculation of suspended particulate matter in NC/Nga mice. / Ogino, Keiki; Takahashi, Noriko; Kubo, Masayuki; Takeuchi, Akihito; Nakagiri, Motoharu; Fujikura, Yoshihisa.

In: Environmental Toxicology, Vol. 29, No. 6, 2014, p. 642-654.

Research output: Contribution to journalArticle

Ogino, K, Takahashi, N, Kubo, M, Takeuchi, A, Nakagiri, M & Fujikura, Y 2014, 'Inflammatory airway responses by nasal inoculation of suspended particulate matter in NC/Nga mice', Environmental Toxicology, vol. 29, no. 6, pp. 642-654. https://doi.org/10.1002/tox.21791
Ogino, Keiki ; Takahashi, Noriko ; Kubo, Masayuki ; Takeuchi, Akihito ; Nakagiri, Motoharu ; Fujikura, Yoshihisa. / Inflammatory airway responses by nasal inoculation of suspended particulate matter in NC/Nga mice. In: Environmental Toxicology. 2014 ; Vol. 29, No. 6. pp. 642-654.
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