Infectivity of human T-lymphotropic virus type I to human nervous tissue cells in vitro

T. Akagi, Y. Hoshida, T. Yoshino, N. Teramoto, E. Kondo, K. Hayashi, K. Takahashi

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21 Citations (Scopus)

Abstract

Infectivity of human T-lymphotropic virus type I (HTLV-I) to human nervous tissue cells was explored using co-cultivation with X-irradiated, HTLV-I-producing MT2 cells. Examined cells included normal cerebellar cells, brain tumor cells (astrocytoma, medulloblastoma, meningioma, hemangioblastoma, and schwannoma), and various cell lines (astrocytoma, ependymoma, oligodendroglioma, medulloblastoma, and neuroblastoma). Successful HTLV-I infection was confirmed immunohistochemically using monoclonal antibodies to HTLV-I p19, p24, and pX product. All cell lines and primary cultures from normal cerebellar tissues and brain tumors could be infected with HTLV-I. Double immunostaining showed that glial fibrillary acidic protein-, S-100 protein- or vimentin-positive cells were susceptible to infection. Neurofilament- or neuronspecific enolase-positive cells in medulloblastoma could also be infected. Reverse-transcriptase assay revealed the productive infection in U251-MG (astrocytoma) and KG-IC (oligodendroglioma) lines. Co-cultivated U251-MG cells formed syncytial polykaryons after serial passages, and polymerase chain reaction assay detected HTLV-I genome in U251-MG syncytial polykaryons and p19+ mononuclear cells. HTLV-I viral RNA was also detected in infected U251-MG cells by in situ hybridization. These data show that HTLV-I may have a wide spectrum of infectivity in human nervous tissues.

Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalActa neuropathologica
Volume84
Issue number2
DOIs
Publication statusPublished - Jul 1 1992

Keywords

  • HTLV-I
  • HTLV-I-associated myopathy/tropical spastic paraparesis
  • Nervous tissue
  • Polymerase chain reaction
  • Tissue culture

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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    Akagi, T., Hoshida, Y., Yoshino, T., Teramoto, N., Kondo, E., Hayashi, K., & Takahashi, K. (1992). Infectivity of human T-lymphotropic virus type I to human nervous tissue cells in vitro. Acta neuropathologica, 84(2), 147-152. https://doi.org/10.1007/BF00311387