Inductions of Cu/Zn superoxide dismutase- and nitric oxide synthase-like immunoreactivities in rabbit spinal cord after transient ischemia

M. Watanabe, M. Sakurai, Koji Abe, M. Aoki, M. Sadahiro, K. Tabayashi, K. Okamoto, M. Shoji, Y. Itoyama

Research output: Contribution to journalArticle

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Abstract

The distributions and inductions of Cu/Zn superoxide dismutase (SOD), neuronal and endothelial nitric oxide (NO) synthase (nNOS and eNOS), and nitrotyrosine (NT) were immunohistochemically examined in rabbit spinal cords after 5 and 15 min of transient ischemia. The neurons in the anterior horns (AH) were selectively lost 7 days after 15-min ischemia as compared with those of sham-operated controls. In the normal spinal cords, a number of neurons in the AHs were positive for the nNOS, and only slightly positive for the Cu/Zn SOD and the eNOS. Immunoreactivities for the proteins were induced at 8-24 h both after 5- and 15-min ischemia. In contrast, NT-like immunoreactivity was negative both in the normal and postischemic spinal cords. These results suggest that Cu/Zn SOD- and nNOS-, and eNOS-like immunoreactivities are induced, but that, even though an interaction of Cu/Zn SOD with NO could be present, NT was not detected in the motor neurons in the rabbit spinal cords after transient ischemia. Other factors could be required for NT formation found in degenerative motor neuron death in humans.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalBrain Research
Volume732
Issue number1-2
DOIs
Publication statusPublished - Sep 2 1996
Externally publishedYes

Fingerprint

Nitric Oxide Synthase
Spinal Cord
Ischemia
Rabbits
Motor Neurons
Anterior Horn Cells
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type III
Nitric Oxide
Neurons
3-nitrotyrosine
Superoxide Dismutase-1
Proteins

Keywords

  • Cu/Zn superoxide dismutase
  • motor neuron death
  • nitric oxide synthase
  • nitrotyrosine
  • rabbit
  • spinal ischemia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inductions of Cu/Zn superoxide dismutase- and nitric oxide synthase-like immunoreactivities in rabbit spinal cord after transient ischemia. / Watanabe, M.; Sakurai, M.; Abe, Koji; Aoki, M.; Sadahiro, M.; Tabayashi, K.; Okamoto, K.; Shoji, M.; Itoyama, Y.

In: Brain Research, Vol. 732, No. 1-2, 02.09.1996, p. 69-74.

Research output: Contribution to journalArticle

Watanabe, M, Sakurai, M, Abe, K, Aoki, M, Sadahiro, M, Tabayashi, K, Okamoto, K, Shoji, M & Itoyama, Y 1996, 'Inductions of Cu/Zn superoxide dismutase- and nitric oxide synthase-like immunoreactivities in rabbit spinal cord after transient ischemia', Brain Research, vol. 732, no. 1-2, pp. 69-74. https://doi.org/10.1016/0006-8993(96)00490-8
Watanabe, M. ; Sakurai, M. ; Abe, Koji ; Aoki, M. ; Sadahiro, M. ; Tabayashi, K. ; Okamoto, K. ; Shoji, M. ; Itoyama, Y. / Inductions of Cu/Zn superoxide dismutase- and nitric oxide synthase-like immunoreactivities in rabbit spinal cord after transient ischemia. In: Brain Research. 1996 ; Vol. 732, No. 1-2. pp. 69-74.
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