Inductions of 3-L-nitrotyrosine in motor neurons after transient spinal cord ischemia in rabbits

Masahiro Sakurai, Naoto Fukuyama, Shunya Takizawa, Koji Abe, Takeshi Hayashi, Yukihito Shinohara, Hiroe Nakazawa, Koichi Tabayashi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The induction and distribution of 3-L-nitrotyrosine (NO2-Tyr) were examined with HPLC and immunohistochemistry in rabbit spinal cords after 15 minutes of transient ischemia until 7 days of the reperfusion. After the 15- minute ischemia, there was a significant decrease of neurologic scores in the ischemic group compared with the sham-operated control group at 7 days of reperfusion (P = 0.0017), and the majority of motor neurons was selectively lost at 7 days of reperfusion (P = 0.0039). NO2-Tyr was transiently induced at 8 hours of reperfusion in the ventral part of the spinal cord (0.47% ± 0.86%, NO2-Tyr/total tyrosine; P = 0.0021), but was not induced at any time point of reperfusion in the dorsal part of the spinal cord. Strong immunoreactivity for NO2-Tyr was selectively induced in large pyramidal motor neurons at 8 hours of reperfusion and was still weakly present until 7 days of reperfusion. (There may be a difference in sensitivity between the two techniques). These results suggested that protein tyrosine nitration by nitric oxide plays a role in the selective motor neuron cell damage after transient spinal cord ischemia.

Original languageEnglish
Pages (from-to)1233-1238
Number of pages6
JournalJournal of Cerebral Blood Flow and Metabolism
Volume18
Issue number11
Publication statusPublished - Nov 1998
Externally publishedYes

Fingerprint

Spinal Cord Ischemia
Motor Neurons
Reperfusion
Rabbits
Spinal Cord
Tyrosine
Ischemia
Pyramidal Cells
3-nitrotyrosine
Nervous System
Nitric Oxide
Immunohistochemistry
High Pressure Liquid Chromatography
Control Groups

Keywords

  • 3-L-Nitrotyrosine
  • Rabbit
  • Spinal cord ischemia

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Sakurai, M., Fukuyama, N., Takizawa, S., Abe, K., Hayashi, T., Shinohara, Y., ... Tabayashi, K. (1998). Inductions of 3-L-nitrotyrosine in motor neurons after transient spinal cord ischemia in rabbits. Journal of Cerebral Blood Flow and Metabolism, 18(11), 1233-1238.

Inductions of 3-L-nitrotyrosine in motor neurons after transient spinal cord ischemia in rabbits. / Sakurai, Masahiro; Fukuyama, Naoto; Takizawa, Shunya; Abe, Koji; Hayashi, Takeshi; Shinohara, Yukihito; Nakazawa, Hiroe; Tabayashi, Koichi.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 18, No. 11, 11.1998, p. 1233-1238.

Research output: Contribution to journalArticle

Sakurai, M, Fukuyama, N, Takizawa, S, Abe, K, Hayashi, T, Shinohara, Y, Nakazawa, H & Tabayashi, K 1998, 'Inductions of 3-L-nitrotyrosine in motor neurons after transient spinal cord ischemia in rabbits', Journal of Cerebral Blood Flow and Metabolism, vol. 18, no. 11, pp. 1233-1238.
Sakurai, Masahiro ; Fukuyama, Naoto ; Takizawa, Shunya ; Abe, Koji ; Hayashi, Takeshi ; Shinohara, Yukihito ; Nakazawa, Hiroe ; Tabayashi, Koichi. / Inductions of 3-L-nitrotyrosine in motor neurons after transient spinal cord ischemia in rabbits. In: Journal of Cerebral Blood Flow and Metabolism. 1998 ; Vol. 18, No. 11. pp. 1233-1238.
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