Inductions of 3-L-nitrotyrosine in motor neurons after transient spinal cord ischemia in rabbits

Masahiro Sakurai, Naoto Fukuyama, Shunya Takizawa, Koji Abe, Takeshi Hayashi, Yukihito Shinohara, Hiroe Nakazawa, Koichi Tabayashi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The induction and distribution of 3-L-nitrotyrosine (NO2-Tyr) were examined with HPLC and immunohistochemistry in rabbit spinal cords after 15 minutes of transient ischemia until 7 days of the reperfusion. After the 15- minute ischemia, there was a significant decrease of neurologic scores in the ischemic group compared with the sham-operated control group at 7 days of reperfusion (P = 0.0017), and the majority of motor neurons was selectively lost at 7 days of reperfusion (P = 0.0039). NO2-Tyr was transiently induced at 8 hours of reperfusion in the ventral part of the spinal cord (0.47% ± 0.86%, NO2-Tyr/total tyrosine; P = 0.0021), but was not induced at any time point of reperfusion in the dorsal part of the spinal cord. Strong immunoreactivity for NO2-Tyr was selectively induced in large pyramidal motor neurons at 8 hours of reperfusion and was still weakly present until 7 days of reperfusion. (There may be a difference in sensitivity between the two techniques). These results suggested that protein tyrosine nitration by nitric oxide plays a role in the selective motor neuron cell damage after transient spinal cord ischemia.

Original languageEnglish
Pages (from-to)1233-1238
Number of pages6
JournalJournal of Cerebral Blood Flow and Metabolism
Volume18
Issue number11
DOIs
Publication statusPublished - Nov 1998
Externally publishedYes

    Fingerprint

Keywords

  • 3-L-Nitrotyrosine
  • Rabbit
  • Spinal cord ischemia

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this