Induction of zinc transporters by forskolin in human trophoblast BeWo cells

Nagayoshi Asano, Masuo Kondoh, Chiaki Ebihara, Makiko Fujii, Tsuyoshi Nakanishi, Naoki Utoguchi, Shuichi Enomoto, Keiichi Tanaka, Yoshiteru Watanabe

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


During pregnancy, the zinc level in fetal serum is up to two-fold higher than that in the maternal serum at the end of pregnancy, but the mechanism of zinc release from the placenta into fetal circulation is not well understood. In this study, we determined the expression profiles of zinc transporters in human trophoblast BeWo cells, a representative human trophoblast cell line. Zn transporter 1-8 (ZnT1-8), Zrt/IRT-like protein 1 and Zn transporter-like transporter 1 were detected in BeWo cells by reverse transcription-polymerase chain reaction. Forskolin (FK) is a representative inducer of differentiation of BeWo cells cytotrophoblast into syncytiotrophoblast. Treatment of BeWo cells with FK resulted in morphological changes of BeWo cells into syncytiotrophoblast cells and secretion of human chorionic gonadotropin, which is a characteristic of syncytiotrophoblast cells. Treatment of BeWo cells with FK elevated ZnT1, 2 and 4 mRNA levels. These data about expression profiles of ZnTs may be useful for further investigation of placental biology.

Original languageEnglish
Pages (from-to)285-291
Number of pages7
JournalReproductive Toxicology
Issue number3
Publication statusPublished - Apr 2006


  • BeWo cells
  • Forskolin
  • Zinc
  • Zinc transporter

ASJC Scopus subject areas

  • Toxicology


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