Induction of urokinase-type plasminogen activator, interleukin-8 and early growth response-1 by STI571 through activating mitogen activated protein kinase in human small cell lung cancer cells

Chikamasa Yoshida, Kenji Niiya, Masami Niiya, Misako Shibakura, Noboru Asaumi, Mitsune Tanimoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We previously demonstrated the simultaneous induction of urokinase-type plasminogen activator and interleukin-8, a CXC chemokine, in doxorubicin-treated human NCI-H69 small cell lung cancer cells in which extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase might be involved. NCI-H69 cells expressed one of the receptor tyrosine kinases, c-Kit, and STI571 inhibited the cell growth and stem cell factor-induced phosphorylation of c-Kit. We therefore investigated the effects of STI571 on the expression of urokinase-type plasminogen activator and interleukin-8 in NCI-H69 cells. Microarray analysis revealed the gene induction of not only urokinase-type plasminogen activator and interleukin-8, but also early growth response-1 in STI571-treated cells. Treatment with STI571 resulted in the induction of phosphorylation of all three mitogen-activated protein kinases, such as extracellular signal-regulated kinase 1/2, p38 mitogen-activated protein kinase and stress-activated protein kinase/c-jun N-terminal protein kinase. U0126, an inhibitor against extracellular signal-regulated kinase 1/2, however, only inhibited the STI571-induced interleukin-8 accumulation. Urokinase-type plasminogen activator and interleukin-8 are important biological factors in tumor cell regulation; STI571 may therefore influence many aspects of tumor cell biology through inducing urokinase-type plasminogen activator and interleukin-8, in which the induction of early growth response-1 expression and extracellular signal-regulated kinase 1/2 phosphorylation might be involved.

Original languageEnglish
Pages (from-to)425-433
Number of pages9
JournalBlood Coagulation and Fibrinolysis
Volume18
Issue number5
DOIs
Publication statusPublished - Jul 2007

Fingerprint

Small Cell Lung Carcinoma
Urokinase-Type Plasminogen Activator
Mitogen-Activated Protein Kinases
Interleukin-8
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Growth
Phosphorylation
p38 Mitogen-Activated Protein Kinases
CXC Chemokines
Stem Cell Factor
JNK Mitogen-Activated Protein Kinases
Biological Factors
Receptor Protein-Tyrosine Kinases
Microarray Analysis
Heat-Shock Proteins
Doxorubicin
Protein Kinases
Cell Biology
Imatinib Mesylate

Keywords

  • Early growth response-1
  • Interleukin-8
  • Mitogen-activated protein kinase
  • Small cell lung cancer
  • STI571
  • Urokinase-type plasminogen activator

ASJC Scopus subject areas

  • Hematology

Cite this

Induction of urokinase-type plasminogen activator, interleukin-8 and early growth response-1 by STI571 through activating mitogen activated protein kinase in human small cell lung cancer cells. / Yoshida, Chikamasa; Niiya, Kenji; Niiya, Masami; Shibakura, Misako; Asaumi, Noboru; Tanimoto, Mitsune.

In: Blood Coagulation and Fibrinolysis, Vol. 18, No. 5, 07.2007, p. 425-433.

Research output: Contribution to journalArticle

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