Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27

Mohamed Nassef, Takahiko Tawaratsumita, Yumi Oba, Hina Satone, Kei Nakayama, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In this study, individual Japanese flounder were intraperitoneally injected with 2μg tributyltin-d27 (TBT-d27) fish-1. Blood samples were collected on day 7 after injection. TBT-binding protein types 1 and 2 (TBT-bp1, -bp2) in the blood serum were quantified by western blotting analysis. As a result, the concentration of TBT-bp2 in TBT-d27 treated group increased to 220% of that in the solvent control, whereas the TBT-bp1 concentration decreased to 65% of that in the solvent control. Additionally, a positive relationship between the concentrations of TBT-bp2 and TBT was observed in blood sera of wild and cultured flounder. We suggest that TBT-bp2 is produced in response to TBT exposure and may play an important role in fish physiology.

Original languageEnglish
Pages (from-to)412-414
Number of pages3
JournalMarine Pollution Bulletin
Volume62
Issue number2
DOIs
Publication statusPublished - Feb 2011
Externally publishedYes

Fingerprint

Paralichthys olivaceus
tributyltin
blood serum
binding proteins
Blood
blood
Fish
protein
serum
flounder
fish
Western blotting
physiology
Physiology
injection
sampling
exposure
Carrier Proteins

Keywords

  • Protein induction
  • Tributyltin-binding proteins

ASJC Scopus subject areas

  • Aquatic Science
  • Oceanography
  • Pollution

Cite this

Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27. / Nassef, Mohamed; Tawaratsumita, Takahiko; Oba, Yumi; Satone, Hina; Nakayama, Kei; Shimasaki, Yohei; Honjo, Tsuneo; Oshima, Yuji.

In: Marine Pollution Bulletin, Vol. 62, No. 2, 02.2011, p. 412-414.

Research output: Contribution to journalArticle

Nassef, M, Tawaratsumita, T, Oba, Y, Satone, H, Nakayama, K, Shimasaki, Y, Honjo, T & Oshima, Y 2011, 'Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27', Marine Pollution Bulletin, vol. 62, no. 2, pp. 412-414. https://doi.org/10.1016/j.marpolbul.2010.12.005
Nassef, Mohamed ; Tawaratsumita, Takahiko ; Oba, Yumi ; Satone, Hina ; Nakayama, Kei ; Shimasaki, Yohei ; Honjo, Tsuneo ; Oshima, Yuji. / Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27. In: Marine Pollution Bulletin. 2011 ; Vol. 62, No. 2. pp. 412-414.
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