Induction of the 'zinc finger' gene after transient focal ischemia in rat cerebral cortex

Koji Abe, Jun ichi Kawagoe, Shigeru Sato, Masaki Sahara, Kyuya Kogure

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

An essential part of gene expression and regulation is the binding of a regulatory protein (transcription factor) to the recognition sequence of the appropriate gene. A novel protein motif for nucleic acid recognition (called 'zinc finger') is one of such transcription factors. A relationship between gene expressions of a transcription factor and heat shock protein (HSP) 70 has been suggested. Possible inductions of mRNA for 'zinc finger' and HSP70 were examined after transient focal ischemia in rat cerebral cortex by Northern blot analysis using a synthetic oligonucleotide probe for 'zinc finger' gene expression, and a human genomic DNA probe for HSP70 gene expression. After 30 min of middle cerebral artery (MCA) occlusion, the rats recovered for 1, 3, 8 h, 1, 2, and 7 days (n = 5). Zinc finger gene is normally expressed in rat cerebral cortex, and is induced by transient ischemia with a maximum at 1 h after the reperfusion. In contrast, HSP70 mRNA is not expressed in normal condition, but is greatly induced by transient ischemia with a maximum at 8 h of reperfusion. These results indicate that the gene expression for a transcription factor changes in the early stage of reperfusion after cerebral ischemia before HSP70 induction begins.

Original languageEnglish
Pages (from-to)248-250
Number of pages3
JournalNeuroscience Letters
Volume123
Issue number2
DOIs
Publication statusPublished - Feb 25 1991
Externally publishedYes

Fingerprint

Zinc Fingers
Cerebral Cortex
Transcription Factors
Ischemia
Reperfusion
Gene Expression
Genes
Messenger RNA
Amino Acid Motifs
HSP70 Heat-Shock Proteins
Oligonucleotide Probes
Middle Cerebral Artery Infarction
Essential Genes
DNA Probes
Gene Expression Regulation
Brain Ischemia
Northern Blotting
Nucleic Acids
Proteins

Keywords

  • Heat shock protein
  • Ischemia
  • Rat
  • Zinc finger

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Induction of the 'zinc finger' gene after transient focal ischemia in rat cerebral cortex. / Abe, Koji; Kawagoe, Jun ichi; Sato, Shigeru; Sahara, Masaki; Kogure, Kyuya.

In: Neuroscience Letters, Vol. 123, No. 2, 25.02.1991, p. 248-250.

Research output: Contribution to journalArticle

Abe, Koji ; Kawagoe, Jun ichi ; Sato, Shigeru ; Sahara, Masaki ; Kogure, Kyuya. / Induction of the 'zinc finger' gene after transient focal ischemia in rat cerebral cortex. In: Neuroscience Letters. 1991 ; Vol. 123, No. 2. pp. 248-250.
@article{070e619313734853a4f3d7736f3d4756,
title = "Induction of the 'zinc finger' gene after transient focal ischemia in rat cerebral cortex",
abstract = "An essential part of gene expression and regulation is the binding of a regulatory protein (transcription factor) to the recognition sequence of the appropriate gene. A novel protein motif for nucleic acid recognition (called 'zinc finger') is one of such transcription factors. A relationship between gene expressions of a transcription factor and heat shock protein (HSP) 70 has been suggested. Possible inductions of mRNA for 'zinc finger' and HSP70 were examined after transient focal ischemia in rat cerebral cortex by Northern blot analysis using a synthetic oligonucleotide probe for 'zinc finger' gene expression, and a human genomic DNA probe for HSP70 gene expression. After 30 min of middle cerebral artery (MCA) occlusion, the rats recovered for 1, 3, 8 h, 1, 2, and 7 days (n = 5). Zinc finger gene is normally expressed in rat cerebral cortex, and is induced by transient ischemia with a maximum at 1 h after the reperfusion. In contrast, HSP70 mRNA is not expressed in normal condition, but is greatly induced by transient ischemia with a maximum at 8 h of reperfusion. These results indicate that the gene expression for a transcription factor changes in the early stage of reperfusion after cerebral ischemia before HSP70 induction begins.",
keywords = "Heat shock protein, Ischemia, Rat, Zinc finger",
author = "Koji Abe and Kawagoe, {Jun ichi} and Shigeru Sato and Masaki Sahara and Kyuya Kogure",
year = "1991",
month = "2",
day = "25",
doi = "10.1016/0304-3940(91)90942-M",
language = "English",
volume = "123",
pages = "248--250",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Induction of the 'zinc finger' gene after transient focal ischemia in rat cerebral cortex

AU - Abe, Koji

AU - Kawagoe, Jun ichi

AU - Sato, Shigeru

AU - Sahara, Masaki

AU - Kogure, Kyuya

PY - 1991/2/25

Y1 - 1991/2/25

N2 - An essential part of gene expression and regulation is the binding of a regulatory protein (transcription factor) to the recognition sequence of the appropriate gene. A novel protein motif for nucleic acid recognition (called 'zinc finger') is one of such transcription factors. A relationship between gene expressions of a transcription factor and heat shock protein (HSP) 70 has been suggested. Possible inductions of mRNA for 'zinc finger' and HSP70 were examined after transient focal ischemia in rat cerebral cortex by Northern blot analysis using a synthetic oligonucleotide probe for 'zinc finger' gene expression, and a human genomic DNA probe for HSP70 gene expression. After 30 min of middle cerebral artery (MCA) occlusion, the rats recovered for 1, 3, 8 h, 1, 2, and 7 days (n = 5). Zinc finger gene is normally expressed in rat cerebral cortex, and is induced by transient ischemia with a maximum at 1 h after the reperfusion. In contrast, HSP70 mRNA is not expressed in normal condition, but is greatly induced by transient ischemia with a maximum at 8 h of reperfusion. These results indicate that the gene expression for a transcription factor changes in the early stage of reperfusion after cerebral ischemia before HSP70 induction begins.

AB - An essential part of gene expression and regulation is the binding of a regulatory protein (transcription factor) to the recognition sequence of the appropriate gene. A novel protein motif for nucleic acid recognition (called 'zinc finger') is one of such transcription factors. A relationship between gene expressions of a transcription factor and heat shock protein (HSP) 70 has been suggested. Possible inductions of mRNA for 'zinc finger' and HSP70 were examined after transient focal ischemia in rat cerebral cortex by Northern blot analysis using a synthetic oligonucleotide probe for 'zinc finger' gene expression, and a human genomic DNA probe for HSP70 gene expression. After 30 min of middle cerebral artery (MCA) occlusion, the rats recovered for 1, 3, 8 h, 1, 2, and 7 days (n = 5). Zinc finger gene is normally expressed in rat cerebral cortex, and is induced by transient ischemia with a maximum at 1 h after the reperfusion. In contrast, HSP70 mRNA is not expressed in normal condition, but is greatly induced by transient ischemia with a maximum at 8 h of reperfusion. These results indicate that the gene expression for a transcription factor changes in the early stage of reperfusion after cerebral ischemia before HSP70 induction begins.

KW - Heat shock protein

KW - Ischemia

KW - Rat

KW - Zinc finger

UR - http://www.scopus.com/inward/record.url?scp=0026092927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026092927&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(91)90942-M

DO - 10.1016/0304-3940(91)90942-M

M3 - Article

C2 - 2027539

AN - SCOPUS:0026092927

VL - 123

SP - 248

EP - 250

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2

ER -