Induction of the expressions of antioxidant enzymes by luteinizing hormone in the bovine corpus luteum

Syota Kawaguchi, Ryosuke Sakumoto, Kiyoshi Okuda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Luteoprotective mechanisms of luteinizing hormone (LH) involved in the maintenance of bovine corpus luteum (CL) function have not been completely clarified. Since antioxidant enzymes are well documented as antiapoptotic factors in the CL of many mammals, we hypothesized that the luteoprotective action of LH is mediated by stimulating the local production and action of antioxidant enzymes. To test the above hypothesis, in the present study, we examined the mechanisms involved in the luteoprotective actions of LH. Cultured bovine luteal cells obtained from the CL at the mid-luteal stage (days 8-12 of the estrous cycle) were treated with LH (10 ng/ml), onapristone (OP; a specific progesterone receptor antagonist, 100 μM) and diethyldithiocarbamate [DETC; an inhibitor of superoxide dismutase (SOD), 100 μM] for 24 h. LH in combination with or without OP significantly increased the mRNA and protein expressions of manganese SOD (Mn-SOD) and catalase (CATA) and SOD activity. While LH alone significantly increased the mRNA and protein expressions of SOD containing copper and zinc (Cu,Zn-SOD), OP in combination with or without LH significantly decreased the mRNA and protein expressions of Cu,Zn-SOD. In addition, Cu,Zn-SOD, Mn-SOD and CATA mRNA expressions were higher at the mid luteal phase than the other luteal phases. LH in combination with DETC significantly decreased LH-increased cell viability. The overall results suggest that LH increases cell viability by LH-increased antioxidant enzymes, resulting in maintenance of CL function during the luteal phase in cattle.

Original languageEnglish
Pages (from-to)219-224
Number of pages6
JournalJournal of Reproduction and Development
Volume59
Issue number3
Publication statusPublished - 2013

Fingerprint

luteinizing hormone
corpus luteum
antioxidants
superoxide dismutase
cattle
enzymes
protein synthesis
cell viability
manganese
catalase
luteal cells
estrous cycle
antagonists
copper
zinc
mammals

Keywords

  • Catalase
  • Luteinizing hormone
  • Progesterone
  • Reactive oxygen species
  • Superoxide dismutase

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Induction of the expressions of antioxidant enzymes by luteinizing hormone in the bovine corpus luteum. / Kawaguchi, Syota; Sakumoto, Ryosuke; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 59, No. 3, 2013, p. 219-224.

Research output: Contribution to journalArticle

Kawaguchi, Syota ; Sakumoto, Ryosuke ; Okuda, Kiyoshi. / Induction of the expressions of antioxidant enzymes by luteinizing hormone in the bovine corpus luteum. In: Journal of Reproduction and Development. 2013 ; Vol. 59, No. 3. pp. 219-224.
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