Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus

Miwako Todoroki, Yoichi Ueta, Hiroaki Fujihara, Hiroki Otsubo, Minori Shibata, Hirofumi Hashimoto, Mizuki Kobayashi, Hirotaka Sakamoto, Mitsuhiro Kawata, Govindan Dayanithi, David Murphy, Hisanori Hiro, Ken Takahashi, Shoji Nagata

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We examined the effects of intracerebroventricular (i.c.v.) administration of colchicine on the expression of the arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene in rats. In rats administered i.c.v. vehicle (control), eGFP fluorescence was observed in the supraoptic nucleus (SON), the magnocellular division of the paraventricular nucleus (PVN), the suprachiasmatic nucleus (SCN), the median eminence (ME) and the posterior pituitary. Two days after i.c.v. administration of colchicine, eGFP fluorescence was markedly increased in the SON, the magnocellular and parvocellular divisions of the PVN, the SCN, the ME and the locus coeruleus (LC). Immunohistochemical staining for eGFP confirmed the distribution of fluorescence in both groups. In the colchicines-administered groups, immunohistochemistry for tyrosine hydroxylase (TH) revealed that the eGFP fluorescence was co-localised with TH-immunoreactivity in the LC. Similarly, in situ hybridization histochemistry for eGFP mRNA revealed a significant increase in gene expression in the LC, the SON and the PVN 1248 h after administration of colchicine. Our results indicate that the synthesis of AVP-eGFP is upregulated in noradrenergic neurones in the LC after colchicine administration. This implies that AVP and noradrenaline, originating from LC neurones, might play a role in response to chronic stress.

Original languageEnglish
Pages (from-to)281-291
Number of pages11
JournalStress
Volume13
Issue number4
DOIs
Publication statusPublished - Jul 2010
Externally publishedYes

Fingerprint

Locus Coeruleus
Arginine Vasopressin
Transgenes
Colchicine
Supraoptic Nucleus
Paraventricular Hypothalamic Nucleus
Fluorescence
Median Eminence
Suprachiasmatic Nucleus
Tyrosine 3-Monooxygenase
Adrenergic Neurons
Gene Fusion
enhanced green fluorescent protein
In Situ Hybridization
Norepinephrine
Immunohistochemistry
Staining and Labeling
Gene Expression
Neurons
Messenger RNA

Keywords

  • Colchicine
  • Green fluorescent protein
  • Hypothalamus
  • Locus coeruleus
  • Noradrenaline
  • Stress
  • Vasopressin

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems
  • Physiology
  • Psychiatry and Mental health
  • Neuropsychology and Physiological Psychology

Cite this

Todoroki, M., Ueta, Y., Fujihara, H., Otsubo, H., Shibata, M., Hashimoto, H., ... Nagata, S. (2010). Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus. Stress, 13(4), 281-291. https://doi.org/10.3109/10253890903383406

Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus. / Todoroki, Miwako; Ueta, Yoichi; Fujihara, Hiroaki; Otsubo, Hiroki; Shibata, Minori; Hashimoto, Hirofumi; Kobayashi, Mizuki; Sakamoto, Hirotaka; Kawata, Mitsuhiro; Dayanithi, Govindan; Murphy, David; Hiro, Hisanori; Takahashi, Ken; Nagata, Shoji.

In: Stress, Vol. 13, No. 4, 07.2010, p. 281-291.

Research output: Contribution to journalArticle

Todoroki, M, Ueta, Y, Fujihara, H, Otsubo, H, Shibata, M, Hashimoto, H, Kobayashi, M, Sakamoto, H, Kawata, M, Dayanithi, G, Murphy, D, Hiro, H, Takahashi, K & Nagata, S 2010, 'Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus', Stress, vol. 13, no. 4, pp. 281-291. https://doi.org/10.3109/10253890903383406
Todoroki, Miwako ; Ueta, Yoichi ; Fujihara, Hiroaki ; Otsubo, Hiroki ; Shibata, Minori ; Hashimoto, Hirofumi ; Kobayashi, Mizuki ; Sakamoto, Hirotaka ; Kawata, Mitsuhiro ; Dayanithi, Govindan ; Murphy, David ; Hiro, Hisanori ; Takahashi, Ken ; Nagata, Shoji. / Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus. In: Stress. 2010 ; Vol. 13, No. 4. pp. 281-291.
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AU - Otsubo, Hiroki

AU - Shibata, Minori

AU - Hashimoto, Hirofumi

AU - Kobayashi, Mizuki

AU - Sakamoto, Hirotaka

AU - Kawata, Mitsuhiro

AU - Dayanithi, Govindan

AU - Murphy, David

AU - Hiro, Hisanori

AU - Takahashi, Ken

AU - Nagata, Shoji

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