Induction of somatic instability in stable yellow leaf mutant of rice by ion beam irradiation

Masahiko Maekawa, Y. Hase, N. Shikazono, A. Tanaka

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Any class II type active transposons have not been discovered in rice though transposon (mobile element) is very useful for gene isolation in several plant species. In order to capture somatic instability induced by an endogenous active transposon in rice, stable yellow leaf plants derived from a variegated yellow leaf (yl-v) mutant found in F2 of a cross between distantly related rice varieties were irradiated with carbon and helium ion beams. In M1 plants derived from the seeds irradiated with 50 Gy of 220 MeV carbon ions, a variegated yl plant was generated and this plant showed small or large sectors in leaves expanded later. Most of panicle-row M2 lines segregated into variegated and stable yl plants. In total, the ratio of variegated to stable yl plants was 3:1, suggesting that clear variegation observed on M1 plants might be caused by activation of a cryptic inactive autonomous element by carbon ion beam irradiation.

Original languageEnglish
Pages (from-to)579-585
Number of pages7
JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume206
DOIs
Publication statusPublished - May 2003

Fingerprint

rice
leaves
Ion beams
induction
Carbon
ion beams
Irradiation
irradiation
carbon
Helium
helium ions
Chemical elements
genes
Seed
seeds
isolation
ions
sectors
Genes
Chemical activation

Keywords

  • Ion beam
  • Rice
  • Somatic instability
  • Transposon
  • Variegation

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Instrumentation
  • Surfaces and Interfaces

Cite this

Induction of somatic instability in stable yellow leaf mutant of rice by ion beam irradiation. / Maekawa, Masahiko; Hase, Y.; Shikazono, N.; Tanaka, A.

In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol. 206, 05.2003, p. 579-585.

Research output: Contribution to journalArticle

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AB - Any class II type active transposons have not been discovered in rice though transposon (mobile element) is very useful for gene isolation in several plant species. In order to capture somatic instability induced by an endogenous active transposon in rice, stable yellow leaf plants derived from a variegated yellow leaf (yl-v) mutant found in F2 of a cross between distantly related rice varieties were irradiated with carbon and helium ion beams. In M1 plants derived from the seeds irradiated with 50 Gy of 220 MeV carbon ions, a variegated yl plant was generated and this plant showed small or large sectors in leaves expanded later. Most of panicle-row M2 lines segregated into variegated and stable yl plants. In total, the ratio of variegated to stable yl plants was 3:1, suggesting that clear variegation observed on M1 plants might be caused by activation of a cryptic inactive autonomous element by carbon ion beam irradiation.

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