Induction of somatic instability in stable yellow leaf mutant of rice by ion beam irradiation

M. Maekawa, Y. Hase, N. Shikazono, A. Tanaka

    Research output: Contribution to journalConference articlepeer-review

    23 Citations (Scopus)

    Abstract

    Any class II type active transposons have not been discovered in rice though transposon (mobile element) is very useful for gene isolation in several plant species. In order to capture somatic instability induced by an endogenous active transposon in rice, stable yellow leaf plants derived from a variegated yellow leaf (yl-v) mutant found in F2 of a cross between distantly related rice varieties were irradiated with carbon and helium ion beams. In M1 plants derived from the seeds irradiated with 50 Gy of 220 MeV carbon ions, a variegated yl plant was generated and this plant showed small or large sectors in leaves expanded later. Most of panicle-row M2 lines segregated into variegated and stable yl plants. In total, the ratio of variegated to stable yl plants was 3:1, suggesting that clear variegation observed on M1 plants might be caused by activation of a cryptic inactive autonomous element by carbon ion beam irradiation.

    Original languageEnglish
    Pages (from-to)579-585
    Number of pages7
    JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
    Volume206
    DOIs
    Publication statusPublished - May 2003
    Event13th International conference on Ion beam modification of Mate - Kobe, Japan
    Duration: Sep 1 2002Sep 6 2002

    Keywords

    • Ion beam
    • Rice
    • Somatic instability
    • Transposon
    • Variegation

    ASJC Scopus subject areas

    • Nuclear and High Energy Physics
    • Instrumentation

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