Induction of proteins in response to cold acclimation of rainbow trout cells

Michiaki Yamashita, Nobuhiko Ojima, Tatsuya Sakamoto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The in vitro translation of poly(A)-RNA isolated from control and cold-treated cells showed that low temperatures induced changes in the population of translatable mRNAs. When cellular proteins extracted from cold-treated cells were subjected to two-dimensional gel electrophoresis, the 70 kDa protein was found to be synthesized during the cold treatment. N-terminal sequence analysis showed that the 70 kDa cold-inducible protein was a homolog of the mammalian valosin-containing protein and yeast CDC48p. The changes in mRNA and protein contents during cold acclimation may result from the expression of genes involved in the adjustment of cellular metabolism to low temperature or the induced proteins may be directly involved in the cold acclimation.

Original languageEnglish
Pages (from-to)261-264
Number of pages4
JournalFEBS Letters
Volume382
Issue number3
DOIs
Publication statusPublished - Mar 18 1996
Externally publishedYes

Fingerprint

Oncorhynchus mykiss
Acclimatization
Proteins
Messenger RNA
Cells
Electrophoresis
Temperature
Metabolism
Yeast
Electrophoresis, Gel, Two-Dimensional
Genes
Gels
Sequence Analysis
Yeasts
Gene Expression
Population

Keywords

  • CDC48
  • Cold acclimation
  • Rainbow trout
  • RTG-2 cell
  • Valosin-containing protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Induction of proteins in response to cold acclimation of rainbow trout cells. / Yamashita, Michiaki; Ojima, Nobuhiko; Sakamoto, Tatsuya.

In: FEBS Letters, Vol. 382, No. 3, 18.03.1996, p. 261-264.

Research output: Contribution to journalArticle

Yamashita, Michiaki ; Ojima, Nobuhiko ; Sakamoto, Tatsuya. / Induction of proteins in response to cold acclimation of rainbow trout cells. In: FEBS Letters. 1996 ; Vol. 382, No. 3. pp. 261-264.
@article{2c28eb48fc344177815a7c61fc28e70a,
title = "Induction of proteins in response to cold acclimation of rainbow trout cells",
abstract = "The in vitro translation of poly(A)-RNA isolated from control and cold-treated cells showed that low temperatures induced changes in the population of translatable mRNAs. When cellular proteins extracted from cold-treated cells were subjected to two-dimensional gel electrophoresis, the 70 kDa protein was found to be synthesized during the cold treatment. N-terminal sequence analysis showed that the 70 kDa cold-inducible protein was a homolog of the mammalian valosin-containing protein and yeast CDC48p. The changes in mRNA and protein contents during cold acclimation may result from the expression of genes involved in the adjustment of cellular metabolism to low temperature or the induced proteins may be directly involved in the cold acclimation.",
keywords = "CDC48, Cold acclimation, Rainbow trout, RTG-2 cell, Valosin-containing protein",
author = "Michiaki Yamashita and Nobuhiko Ojima and Tatsuya Sakamoto",
year = "1996",
month = "3",
day = "18",
doi = "10.1016/0014-5793(96)00172-X",
language = "English",
volume = "382",
pages = "261--264",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Induction of proteins in response to cold acclimation of rainbow trout cells

AU - Yamashita, Michiaki

AU - Ojima, Nobuhiko

AU - Sakamoto, Tatsuya

PY - 1996/3/18

Y1 - 1996/3/18

N2 - The in vitro translation of poly(A)-RNA isolated from control and cold-treated cells showed that low temperatures induced changes in the population of translatable mRNAs. When cellular proteins extracted from cold-treated cells were subjected to two-dimensional gel electrophoresis, the 70 kDa protein was found to be synthesized during the cold treatment. N-terminal sequence analysis showed that the 70 kDa cold-inducible protein was a homolog of the mammalian valosin-containing protein and yeast CDC48p. The changes in mRNA and protein contents during cold acclimation may result from the expression of genes involved in the adjustment of cellular metabolism to low temperature or the induced proteins may be directly involved in the cold acclimation.

AB - The in vitro translation of poly(A)-RNA isolated from control and cold-treated cells showed that low temperatures induced changes in the population of translatable mRNAs. When cellular proteins extracted from cold-treated cells were subjected to two-dimensional gel electrophoresis, the 70 kDa protein was found to be synthesized during the cold treatment. N-terminal sequence analysis showed that the 70 kDa cold-inducible protein was a homolog of the mammalian valosin-containing protein and yeast CDC48p. The changes in mRNA and protein contents during cold acclimation may result from the expression of genes involved in the adjustment of cellular metabolism to low temperature or the induced proteins may be directly involved in the cold acclimation.

KW - CDC48

KW - Cold acclimation

KW - Rainbow trout

KW - RTG-2 cell

KW - Valosin-containing protein

UR - http://www.scopus.com/inward/record.url?scp=0030001771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030001771&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(96)00172-X

DO - 10.1016/0014-5793(96)00172-X

M3 - Article

C2 - 8605981

AN - SCOPUS:0030001771

VL - 382

SP - 261

EP - 264

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -