Induction of proteins in response to cold acclimation of rainbow trout cells

Michiaki Yamashita, Nobuhiko Ojima, Tatsuya Sakamoto

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


The in vitro translation of poly(A)-RNA isolated from control and cold-treated cells showed that low temperatures induced changes in the population of translatable mRNAs. When cellular proteins extracted from cold-treated cells were subjected to two-dimensional gel electrophoresis, the 70 kDa protein was found to be synthesized during the cold treatment. N-terminal sequence analysis showed that the 70 kDa cold-inducible protein was a homolog of the mammalian valosin-containing protein and yeast CDC48p. The changes in mRNA and protein contents during cold acclimation may result from the expression of genes involved in the adjustment of cellular metabolism to low temperature or the induced proteins may be directly involved in the cold acclimation.

Original languageEnglish
Pages (from-to)261-264
Number of pages4
JournalFEBS Letters
Issue number3
Publication statusPublished - Mar 18 1996
Externally publishedYes


  • CDC48
  • Cold acclimation
  • RTG-2 cell
  • Rainbow trout
  • Valosin-containing protein

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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