Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51

Keita Miki, Toshi Nagata, Takao Tanaka, Yeung Hyen Kim, Masato Uchijima, Naoya Oohara, Satoshi Nakamura, Masaji Okada, Yukio Koide

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We report here the induction of specific protective cellular immunity against Mycobacterium tuberculosis by the employment of vaccination with recombinant attenuated Listeria monocytogenes strains. We constructed self-destructing attenuated L. monocytogenes Δ2 strains carrying eukaryotic expression plasmids for the antigen 85 complex (Ag85A and Ag85B) and for MPB/MPT51 (mycobacterial protein secreted by M. bovis BCG/mycobacterial protein secreted by M. tuberculosis) molecules. Infection of these recombinant bacteria allowed expression of the genes in the J774A.1 murine macrophage cell line. Intraperitoneal vaccination of C57BL/6 mice with these recombinant bacteria was capable of inducing purified protein derivative-specific cellular immune responses, such as foot pad reactions, proliferative responses of splenocytes, and gamma interferon production from splenocytes, suggesting the efficacy of vaccination against mycobacterial infection by use of these recombinant L. monocytogenes strains. Furthermore, intravenons vaccination with recombinant bacteria carrying expression plasmids for Ag85A, Ag85B, or MPB/ MPT51 in BALB/c mice elicited significant protective responses, comparable to those evoked by a live Mycobacterium bovis BCG vaccine. Notably, this is the first report to show that MPB/MPT51 is a major protective antigen in addition to Ag85A and Ag85B, which have been reported to be major mycobacterial protective antigens.

Original languageEnglish
Pages (from-to)2014-2021
Number of pages8
JournalInfection and Immunity
Volume72
Issue number4
DOIs
Publication statusPublished - Apr 2004
Externally publishedYes

Fingerprint

Listeria monocytogenes
Mycobacterium tuberculosis
Cellular Immunity
Vaccination
Plasmids
Antigens
Mycobacterium bovis
Bacteria
BCG Vaccine
Proteins
Infection
Inbred C57BL Mouse
Interferon-gamma
Foot
Macrophages
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51. / Miki, Keita; Nagata, Toshi; Tanaka, Takao; Kim, Yeung Hyen; Uchijima, Masato; Oohara, Naoya; Nakamura, Satoshi; Okada, Masaji; Koide, Yukio.

In: Infection and Immunity, Vol. 72, No. 4, 04.2004, p. 2014-2021.

Research output: Contribution to journalArticle

Miki, Keita ; Nagata, Toshi ; Tanaka, Takao ; Kim, Yeung Hyen ; Uchijima, Masato ; Oohara, Naoya ; Nakamura, Satoshi ; Okada, Masaji ; Koide, Yukio. / Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51. In: Infection and Immunity. 2004 ; Vol. 72, No. 4. pp. 2014-2021.
@article{bda23e97a17f44a7bdf03576c46e63cf,
title = "Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51",
abstract = "We report here the induction of specific protective cellular immunity against Mycobacterium tuberculosis by the employment of vaccination with recombinant attenuated Listeria monocytogenes strains. We constructed self-destructing attenuated L. monocytogenes Δ2 strains carrying eukaryotic expression plasmids for the antigen 85 complex (Ag85A and Ag85B) and for MPB/MPT51 (mycobacterial protein secreted by M. bovis BCG/mycobacterial protein secreted by M. tuberculosis) molecules. Infection of these recombinant bacteria allowed expression of the genes in the J774A.1 murine macrophage cell line. Intraperitoneal vaccination of C57BL/6 mice with these recombinant bacteria was capable of inducing purified protein derivative-specific cellular immune responses, such as foot pad reactions, proliferative responses of splenocytes, and gamma interferon production from splenocytes, suggesting the efficacy of vaccination against mycobacterial infection by use of these recombinant L. monocytogenes strains. Furthermore, intravenons vaccination with recombinant bacteria carrying expression plasmids for Ag85A, Ag85B, or MPB/ MPT51 in BALB/c mice elicited significant protective responses, comparable to those evoked by a live Mycobacterium bovis BCG vaccine. Notably, this is the first report to show that MPB/MPT51 is a major protective antigen in addition to Ag85A and Ag85B, which have been reported to be major mycobacterial protective antigens.",
author = "Keita Miki and Toshi Nagata and Takao Tanaka and Kim, {Yeung Hyen} and Masato Uchijima and Naoya Oohara and Satoshi Nakamura and Masaji Okada and Yukio Koide",
year = "2004",
month = "4",
doi = "10.1128/IAI.72.4.2014-2021.2004",
language = "English",
volume = "72",
pages = "2014--2021",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51

AU - Miki, Keita

AU - Nagata, Toshi

AU - Tanaka, Takao

AU - Kim, Yeung Hyen

AU - Uchijima, Masato

AU - Oohara, Naoya

AU - Nakamura, Satoshi

AU - Okada, Masaji

AU - Koide, Yukio

PY - 2004/4

Y1 - 2004/4

N2 - We report here the induction of specific protective cellular immunity against Mycobacterium tuberculosis by the employment of vaccination with recombinant attenuated Listeria monocytogenes strains. We constructed self-destructing attenuated L. monocytogenes Δ2 strains carrying eukaryotic expression plasmids for the antigen 85 complex (Ag85A and Ag85B) and for MPB/MPT51 (mycobacterial protein secreted by M. bovis BCG/mycobacterial protein secreted by M. tuberculosis) molecules. Infection of these recombinant bacteria allowed expression of the genes in the J774A.1 murine macrophage cell line. Intraperitoneal vaccination of C57BL/6 mice with these recombinant bacteria was capable of inducing purified protein derivative-specific cellular immune responses, such as foot pad reactions, proliferative responses of splenocytes, and gamma interferon production from splenocytes, suggesting the efficacy of vaccination against mycobacterial infection by use of these recombinant L. monocytogenes strains. Furthermore, intravenons vaccination with recombinant bacteria carrying expression plasmids for Ag85A, Ag85B, or MPB/ MPT51 in BALB/c mice elicited significant protective responses, comparable to those evoked by a live Mycobacterium bovis BCG vaccine. Notably, this is the first report to show that MPB/MPT51 is a major protective antigen in addition to Ag85A and Ag85B, which have been reported to be major mycobacterial protective antigens.

AB - We report here the induction of specific protective cellular immunity against Mycobacterium tuberculosis by the employment of vaccination with recombinant attenuated Listeria monocytogenes strains. We constructed self-destructing attenuated L. monocytogenes Δ2 strains carrying eukaryotic expression plasmids for the antigen 85 complex (Ag85A and Ag85B) and for MPB/MPT51 (mycobacterial protein secreted by M. bovis BCG/mycobacterial protein secreted by M. tuberculosis) molecules. Infection of these recombinant bacteria allowed expression of the genes in the J774A.1 murine macrophage cell line. Intraperitoneal vaccination of C57BL/6 mice with these recombinant bacteria was capable of inducing purified protein derivative-specific cellular immune responses, such as foot pad reactions, proliferative responses of splenocytes, and gamma interferon production from splenocytes, suggesting the efficacy of vaccination against mycobacterial infection by use of these recombinant L. monocytogenes strains. Furthermore, intravenons vaccination with recombinant bacteria carrying expression plasmids for Ag85A, Ag85B, or MPB/ MPT51 in BALB/c mice elicited significant protective responses, comparable to those evoked by a live Mycobacterium bovis BCG vaccine. Notably, this is the first report to show that MPB/MPT51 is a major protective antigen in addition to Ag85A and Ag85B, which have been reported to be major mycobacterial protective antigens.

UR - http://www.scopus.com/inward/record.url?scp=1842431883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842431883&partnerID=8YFLogxK

U2 - 10.1128/IAI.72.4.2014-2021.2004

DO - 10.1128/IAI.72.4.2014-2021.2004

M3 - Article

C2 - 15039321

AN - SCOPUS:1842431883

VL - 72

SP - 2014

EP - 2021

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 4

ER -