Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits

Masahiro Sakurai, Takae Kawamura, Hidekazu Nishimura, Hiroyoshi Suzuki, Fumiaki Tezuka, Koji Abe

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The mechanism of spinal cord injury has been thought to be related to the vulnerability of spinal motor neuron cells against ischemia. However, the mechanisms of such vulnerability are not fully understood. We investigated a possible mechanism of neuronal death by immunohistochemical analysis for DJ-1, PINK1, and α-Synuclein. We used a 15-min rabbit spinal cord ischemia model, with use of a balloon catheter. Western blot analysis for DJ-1, PINK1, and α-Synuclein; temporal profiles of DJ-1, PINK1, and α-Synuclein immunoreactivity; and double-label fluorescence immunocytochemical studies were performed. Western blot analysis revealed scarce immunoreactivity for DJ-1, PINK1, and α-Synuclein in the sham-operated spinal cords. However, they became apparent at 8 h after transient ischemia, which returned to the baseline level at 1 day. Double-label fluorescence immunocytochemical study revealed that both DJ-1and PINK1, and DJ-1 and α-Synuclein were positive at 8 h of reperfusion in the same motor neurons, which eventually die. The induction of DJ-1 and PINK1 proteins in the motor neurons at the early stage of reperfusion may indicate oxidative stress, and the induction of α-Synuclein may be implicated in the programmed cell death change after transient spinal cord ischemia.

Original languageEnglish
Pages (from-to)752-758
Number of pages7
JournalJournal of Cerebral Blood Flow and Metabolism
Volume29
Issue number4
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Synucleins
Spinal Cord Ischemia
Motor Neurons
Parkinson Disease
Rabbits
Proteins
Reperfusion
Ischemia
Fluorescence
Western Blotting
Spinal Cord Injuries
Spinal Cord
Oxidative Stress
Cell Death
Catheters

Keywords

  • Ischemia
  • Parkinsons disease
  • Spinal cord
  • Stress response

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits. / Sakurai, Masahiro; Kawamura, Takae; Nishimura, Hidekazu; Suzuki, Hiroyoshi; Tezuka, Fumiaki; Abe, Koji.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 29, No. 4, 04.2009, p. 752-758.

Research output: Contribution to journalArticle

Sakurai, Masahiro ; Kawamura, Takae ; Nishimura, Hidekazu ; Suzuki, Hiroyoshi ; Tezuka, Fumiaki ; Abe, Koji. / Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits. In: Journal of Cerebral Blood Flow and Metabolism. 2009 ; Vol. 29, No. 4. pp. 752-758.
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