Induction of oxidative stress by anticancer drugs in the presence and absence of cells

Chikako Yokoyama, Yuto Sueyoshi, Mika Ema, Yumi Mori, Kazuto Takaishi, Hisashi Hisatomi

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) are generated in the cell through multiple mechanisms. Intracellular ROS are rapidly detoxified by various enzymatic and non-enzymatic mechanisms; however, disruption of the oxidant-antioxidant balance causes oxidative stress and elicits cell damage. The oxidative stress induced by chemotherapy is known to cause side effects in patients with cancer. However, few studies have examined whether anticancer drugs induce oxidative stress in cancer cells. Furthermore, the precise mechanism by which anticancer drugs induce the generation of ROS remains unclear. In the present study, to investigate whether anticancer drugs induce oxidative stress, DLD-1 human colorectal cancer cells were treated with 20 different anticancer drugs and then stained with CellROX® ROS detection reagent. Furthermore, an oxygen radical absorbance capacity assay in the presence of copper was performed to estimate the oxidative activities of the anticancer drugs in the absence of cells. The data of the present study using assay methods in the presence and absence of cells suggest that nimustine, actinomycin D, doxorubicin, mitomycin C, mitoxantrone, carmofur, gemcitabine, mercaptopurine, camptothecin, paclitaxel, vinblastine, and vinorelbine are able to induce oxidative stress.

Original languageEnglish
Pages (from-to)6066-6070
Number of pages5
JournalOncology Letters
Volume14
Issue number5
DOIs
Publication statusPublished - Nov 2017

Keywords

  • Anticancer drug
  • CellROX®
  • Oxidative stress
  • Reactive oxygen species
  • The oxygen radical absorbance capacity assay in the presence of copper

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Induction of oxidative stress by anticancer drugs in the presence and absence of cells'. Together they form a unique fingerprint.

  • Cite this