TY - JOUR
T1 - Induction of oviposition by injection of male-derived extracts in two Callosobruchus species
AU - Yamane, Takashi
AU - Miyatake, Takahisa
N1 - Funding Information:
We thank Dr. Yoshinobu Kimura (Okayama University, Okayama, Japan) and Dr. Kenji Matsuura (Okayama University, Okayama, Japan) for obtaining the facilities used for the experiments, Dr. Maki Katsuhara (Research Institute for Bioresources, Okayama University, Kurashiki, Japan) for technical advices on injection, Dr. Shin-ya Ohba (Institute of Tropical Medicine of Nagasaki University, Nagasaki, Japan) and Tomohiro Harano (Kyushu University, Fukuoka, Japan) for statistical advices. This work was supported by KAKENHI 19370011 and 19657026 , Grant-in-Aid for Scientific Research, JSPS and MEXT to T.M., and Research Fellowships for Young Scientists (JSPS 205869) to T.Y.
PY - 2010/12
Y1 - 2010/12
N2 - In some insect species, certain substances in the seminal fluid of males induce egg production and laying in females. We determined the effects of male-derived substances on female oviposition behaviour in two Callosobruchus species, C. chinensis and C. maculatus. Aqueous extracts of the accessory gland; testis; and seminal vesicle, including the ejaculatory duct, were prepared. The injection of these extracts into abdomen of females induced oviposition in both species. Oviposition was induced by the testis and seminal vesicle extracts in C. chinensis and by the accessory gland extracts in C. maculatus. The extracts were separated into three fractions by ultrafiltration: fractions I, molecular weight (MW) <3. kDa; fraction II, 3-14. kDa; and fraction III, >14. kDa. Fraction III induced oviposition in both species. These results suggest that in these two species, the substances that induce oviposition have similar MW but are present in different organs. Oviposition was induced by high-MW (>14. kDa) substances in the testis and seminal vesicle in C. chinensis, and by high-MW substances in accessory gland in C. maculatus. Here, we have discussed the relationship between oviposition and the abovementioned male-derived substances.
AB - In some insect species, certain substances in the seminal fluid of males induce egg production and laying in females. We determined the effects of male-derived substances on female oviposition behaviour in two Callosobruchus species, C. chinensis and C. maculatus. Aqueous extracts of the accessory gland; testis; and seminal vesicle, including the ejaculatory duct, were prepared. The injection of these extracts into abdomen of females induced oviposition in both species. Oviposition was induced by the testis and seminal vesicle extracts in C. chinensis and by the accessory gland extracts in C. maculatus. The extracts were separated into three fractions by ultrafiltration: fractions I, molecular weight (MW) <3. kDa; fraction II, 3-14. kDa; and fraction III, >14. kDa. Fraction III induced oviposition in both species. These results suggest that in these two species, the substances that induce oviposition have similar MW but are present in different organs. Oviposition was induced by high-MW (>14. kDa) substances in the testis and seminal vesicle in C. chinensis, and by high-MW substances in accessory gland in C. maculatus. Here, we have discussed the relationship between oviposition and the abovementioned male-derived substances.
KW - Callosobruchus
KW - Female multiple mating
KW - Induction of oviposition
KW - Male-derived substances
KW - Reproductive organs
KW - Seminal fluid
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U2 - 10.1016/j.jinsphys.2010.07.008
DO - 10.1016/j.jinsphys.2010.07.008
M3 - Article
C2 - 20670630
AN - SCOPUS:77958477181
VL - 56
SP - 1783
EP - 1788
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
SN - 0022-1910
IS - 12
ER -