Induction of nitrotyrosine-like immunoreactivity in the lower motor neuron of amyotrophic lateral sclerosis

K. Abe; L. H. Pan; M. Watanabe; T. Kato; Y. Itoyama

doi:10.1016/0304-3940(95)12039-7

Induction of nitrotyrosine-like immunoreactivity in the lower motor neuron of amyotrophic lateral sclerosis

K. Abe, L. H. Pan, M. Watanabe, T. Kato, Y. Itoyama

Research output: Contribution to journal › Article › peer-review

220 Citations (Scopus)

Abstract

Spinal cords of sporadic cases with amyotrophic lateral sclerosis (ALS) and normal controls were immunohistochemically examined using antibodies for Cu/Zn superoxide dismutase (SOD) and nitrotyrosine (NT). Immunoreactivity for Cu/Zn SOD of the motor neurons was not different between the ALS and controls. In contrast, immunoreactivity for NT was densely detected in motor neurons of ALS but was not or was only minimally detected in those of controls. The staining was also found in the axons of motor neurons of ALS, but was not found in the controls. These results suggest that nitration of protein-tyrosine residue is upregulated in motor neurons of the spinal cord of ALS.

Original language	English
Pages (from-to)	152-154
Number of pages	3
Journal	Neuroscience Letters
Volume	199
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(95)12039-7
Publication status	Published - Oct 20 1995
Externally published	Yes

Keywords

Amyotrophic lateral sclerosis
Nitrotyrosine
Superoxide dismutase

ASJC Scopus subject areas

Neuroscience(all)

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title = "Induction of nitrotyrosine-like immunoreactivity in the lower motor neuron of amyotrophic lateral sclerosis",

abstract = "Spinal cords of sporadic cases with amyotrophic lateral sclerosis (ALS) and normal controls were immunohistochemically examined using antibodies for Cu/Zn superoxide dismutase (SOD) and nitrotyrosine (NT). Immunoreactivity for Cu/Zn SOD of the motor neurons was not different between the ALS and controls. In contrast, immunoreactivity for NT was densely detected in motor neurons of ALS but was not or was only minimally detected in those of controls. The staining was also found in the axons of motor neurons of ALS, but was not found in the controls. These results suggest that nitration of protein-tyrosine residue is upregulated in motor neurons of the spinal cord of ALS.",

keywords = "Amyotrophic lateral sclerosis, Nitrotyrosine, Superoxide dismutase",

author = "K. Abe and Pan, {L. H.} and M. Watanabe and T. Kato and Y. Itoyama",

note = "Funding Information: The authors appreciate cooperation of the patients and their families with this project. We thank J.S. Beckman, T. Siddique, and RH. Brown Jr. for the generous discussion and support. This work was partly supported by Grant-in-Aid for Scientific Research on Priority Areas (Kanazawa I) 06272204 and 07264204, and Grant-in-Aid for Scientific Research (B) 07457152 and (C) 06807055 from the Ministry of Education, Science and Culture of Japan, and by a grant (Hirai S) from the Ministry of Health and Welfare of Japan.",

year = "1995",

month = oct,

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doi = "10.1016/0304-3940(95)12039-7",

language = "English",

volume = "199",

pages = "152--154",

journal = "Neuroscience Letters",

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number = "2",

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TY - JOUR

T1 - Induction of nitrotyrosine-like immunoreactivity in the lower motor neuron of amyotrophic lateral sclerosis

AU - Abe, K.

AU - Pan, L. H.

AU - Watanabe, M.

AU - Kato, T.

AU - Itoyama, Y.

N1 - Funding Information: The authors appreciate cooperation of the patients and their families with this project. We thank J.S. Beckman, T. Siddique, and RH. Brown Jr. for the generous discussion and support. This work was partly supported by Grant-in-Aid for Scientific Research on Priority Areas (Kanazawa I) 06272204 and 07264204, and Grant-in-Aid for Scientific Research (B) 07457152 and (C) 06807055 from the Ministry of Education, Science and Culture of Japan, and by a grant (Hirai S) from the Ministry of Health and Welfare of Japan.

PY - 1995/10/20

Y1 - 1995/10/20

N2 - Spinal cords of sporadic cases with amyotrophic lateral sclerosis (ALS) and normal controls were immunohistochemically examined using antibodies for Cu/Zn superoxide dismutase (SOD) and nitrotyrosine (NT). Immunoreactivity for Cu/Zn SOD of the motor neurons was not different between the ALS and controls. In contrast, immunoreactivity for NT was densely detected in motor neurons of ALS but was not or was only minimally detected in those of controls. The staining was also found in the axons of motor neurons of ALS, but was not found in the controls. These results suggest that nitration of protein-tyrosine residue is upregulated in motor neurons of the spinal cord of ALS.

AB - Spinal cords of sporadic cases with amyotrophic lateral sclerosis (ALS) and normal controls were immunohistochemically examined using antibodies for Cu/Zn superoxide dismutase (SOD) and nitrotyrosine (NT). Immunoreactivity for Cu/Zn SOD of the motor neurons was not different between the ALS and controls. In contrast, immunoreactivity for NT was densely detected in motor neurons of ALS but was not or was only minimally detected in those of controls. The staining was also found in the axons of motor neurons of ALS, but was not found in the controls. These results suggest that nitration of protein-tyrosine residue is upregulated in motor neurons of the spinal cord of ALS.

KW - Amyotrophic lateral sclerosis

KW - Nitrotyrosine

KW - Superoxide dismutase

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AN - SCOPUS:0028971936

VL - 199

SP - 152

EP - 154

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2

ER -

Induction of nitrotyrosine-like immunoreactivity in the lower motor neuron of amyotrophic lateral sclerosis

Abstract

Keywords

ASJC Scopus subject areas

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