Induction of myeloperoxidase and nitrotyrosine formation in a human eosinophilic leukemia cell line, EoL-1

Norio Kodama, Yasuhiro Kambayashi, Masayuki Kubo, Yoshitaka Nobukuni, Shingo Kimura, Hiroyuki Nakamura, Keiki Ogino

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3 Citations (Scopus)

Abstract

The human eosinophilic leukemia cell line, EoL-1, differentiated with butyrate as an eosinophilic cellular model was evaluated for peroxidase-dependent tyrosine nitration. Butyrate suppressed cell growth and induced eosinophilic granules in EoL-1 cells after 9 days of culture. Peroxidase activity was detected biochemically and histochemically from 3-day cultures and it increased in a time dependent manner. This peroxidase activity was inhibited by cyanide. Nitrotyrosine formation catalysed by peroxidase using hydrogen peroxide and nitrite was detected at a high level similar to that of mature eosinophils. However, no expression of eosinophil peroxidase (EPO) was detected by RT-PCR or immunocytochemistry. In contrast, the induction of myeloperoxidase (MPO) by butyrate was clearly detected by RT-PCR, Northern blot, and immunocytochemical staining. These results suggest that butyrate induces MPO rather than EPO in EoL-1 cells and that the formation of nitrotyrosine in butyrate-induced cells is dependent on MPO.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalCell biochemistry and function
Volume22
Issue number2
DOIs
Publication statusPublished - Mar 2004

Keywords

  • Eosinophil peroxidase
  • Myeloperoxidase
  • Nitrotyrosine
  • Reactive nitrogen species

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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    Kodama, N., Kambayashi, Y., Kubo, M., Nobukuni, Y., Kimura, S., Nakamura, H., & Ogino, K. (2004). Induction of myeloperoxidase and nitrotyrosine formation in a human eosinophilic leukemia cell line, EoL-1. Cell biochemistry and function, 22(2), 105-112. https://doi.org/10.1002/cbf.1051