Abstract
The direct effect of osmolality on growth and mRNA population were investigated in the rainbow trout cell line (RTG-2). These cells can grow in the media of osmolalities ranging from 200 to 600 mosmol kg-1. With two-dimensional electrophoresis, the in vitro translation of poly(A+) RNA isolated from these cells showed osmoresponsive changes in the population of translatable mRNAs. Using differential mRNA display polymerase chain reaction, however, we identified inducible cDNA products in hyper-osmotic and hypo-osmotic media as third component of complement, and as homologues of known genes: an atypical protein kinase regulated by the thyrotropin-dependent mitogenic pathway, nucleolin and CHD3. The remaining cDNAs have no significant homology in GenBank. Northern blots demonstrate that their mRNA levels were induced in hyper-osmotic and hypo-osmotic media, but not by other stresses. The expressed proteins of these mRNAs may be involved directly or indirectly in the adaptation of RTG-2 cells to different osmolalities probably through the osmotic signal transduction and adjustment in cellular metabolism to osmotic stress.
| Original language | English |
|---|---|
| Pages (from-to) | 255-262 |
| Number of pages | 8 |
| Journal | Fish Physiology and Biochemistry |
| Volume | 22 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2000 |
| Externally published | Yes |
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Keywords
- Cell culture
- CHD3
- Complement
- Differential mRNA display polymerase chain reaction
- Growth
- Nucleolin
- Protein kinase
- Rainbow trout
- RTG-2 cell
- Salinity
ASJC Scopus subject areas
- Aquatic Science
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Physiology
- Physiology (medical)
Cite this
Induction of mRNAs in response to acclimation of trout cells to different osmolalities. / Sakamoto, Tatsuya; Ojima, N.; Yamashita, M.
In: Fish Physiology and Biochemistry, Vol. 22, No. 3, 2000, p. 255-262.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of mRNAs in response to acclimation of trout cells to different osmolalities
AU - Sakamoto, Tatsuya
AU - Ojima, N.
AU - Yamashita, M.
PY - 2000
Y1 - 2000
N2 - The direct effect of osmolality on growth and mRNA population were investigated in the rainbow trout cell line (RTG-2). These cells can grow in the media of osmolalities ranging from 200 to 600 mosmol kg-1. With two-dimensional electrophoresis, the in vitro translation of poly(A+) RNA isolated from these cells showed osmoresponsive changes in the population of translatable mRNAs. Using differential mRNA display polymerase chain reaction, however, we identified inducible cDNA products in hyper-osmotic and hypo-osmotic media as third component of complement, and as homologues of known genes: an atypical protein kinase regulated by the thyrotropin-dependent mitogenic pathway, nucleolin and CHD3. The remaining cDNAs have no significant homology in GenBank. Northern blots demonstrate that their mRNA levels were induced in hyper-osmotic and hypo-osmotic media, but not by other stresses. The expressed proteins of these mRNAs may be involved directly or indirectly in the adaptation of RTG-2 cells to different osmolalities probably through the osmotic signal transduction and adjustment in cellular metabolism to osmotic stress.
AB - The direct effect of osmolality on growth and mRNA population were investigated in the rainbow trout cell line (RTG-2). These cells can grow in the media of osmolalities ranging from 200 to 600 mosmol kg-1. With two-dimensional electrophoresis, the in vitro translation of poly(A+) RNA isolated from these cells showed osmoresponsive changes in the population of translatable mRNAs. Using differential mRNA display polymerase chain reaction, however, we identified inducible cDNA products in hyper-osmotic and hypo-osmotic media as third component of complement, and as homologues of known genes: an atypical protein kinase regulated by the thyrotropin-dependent mitogenic pathway, nucleolin and CHD3. The remaining cDNAs have no significant homology in GenBank. Northern blots demonstrate that their mRNA levels were induced in hyper-osmotic and hypo-osmotic media, but not by other stresses. The expressed proteins of these mRNAs may be involved directly or indirectly in the adaptation of RTG-2 cells to different osmolalities probably through the osmotic signal transduction and adjustment in cellular metabolism to osmotic stress.
KW - Cell culture
KW - CHD3
KW - Complement
KW - Differential mRNA display polymerase chain reaction
KW - Growth
KW - Nucleolin
KW - Protein kinase
KW - Rainbow trout
KW - RTG-2 cell
KW - Salinity
UR - http://www.scopus.com/inward/record.url?scp=0033928044&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033928044&partnerID=8YFLogxK
U2 - 10.1023/A:1007843508043
DO - 10.1023/A:1007843508043
M3 - Article
VL - 22
SP - 255
EP - 262
JO - Fish Physiology and Biochemistry
JF - Fish Physiology and Biochemistry
SN - 0920-1742
IS - 3
ER -