Induction of mRNAs in response to acclimation of trout cells to different osmolalities

T. Sakamoto, N. Ojima, M. Yamashita

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The direct effect of osmolality on growth and mRNA population were investigated in the rainbow trout cell line (RTG-2). These cells can grow in the media of osmolalities ranging from 200 to 600 mosmol kg-1. With two-dimensional electrophoresis, the in vitro translation of poly(A+) RNA isolated from these cells showed osmoresponsive changes in the population of translatable mRNAs. Using differential mRNA display polymerase chain reaction, however, we identified inducible cDNA products in hyper-osmotic and hypo-osmotic media as third component of complement, and as homologues of known genes: an atypical protein kinase regulated by the thyrotropin-dependent mitogenic pathway, nucleolin and CHD3. The remaining cDNAs have no significant homology in GenBank. Northern blots demonstrate that their mRNA levels were induced in hyper-osmotic and hypo-osmotic media, but not by other stresses. The expressed proteins of these mRNAs may be involved directly or indirectly in the adaptation of RTG-2 cells to different osmolalities probably through the osmotic signal transduction and adjustment in cellular metabolism to osmotic stress.

Original languageEnglish
Pages (from-to)255-262
Number of pages8
JournalFish Physiology and Biochemistry
Volume22
Issue number3
DOIs
Publication statusPublished - Aug 7 2000
Externally publishedYes

Keywords

  • CHD3
  • Cell culture
  • Complement
  • Differential mRNA display polymerase chain reaction
  • Growth
  • Nucleolin
  • Protein kinase
  • RTG-2 cell
  • Rainbow trout
  • Salinity

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Aquatic Science

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