Abstract
The direct effect of osmolality on growth and mRNA population were investigated in the rainbow trout cell line (RTG-2). These cells can grow in the media of osmolalities ranging from 200 to 600 mosmol kg-1. With two-dimensional electrophoresis, the in vitro translation of poly(A+) RNA isolated from these cells showed osmoresponsive changes in the population of translatable mRNAs. Using differential mRNA display polymerase chain reaction, however, we identified inducible cDNA products in hyper-osmotic and hypo-osmotic media as third component of complement, and as homologues of known genes: an atypical protein kinase regulated by the thyrotropin-dependent mitogenic pathway, nucleolin and CHD3. The remaining cDNAs have no significant homology in GenBank. Northern blots demonstrate that their mRNA levels were induced in hyper-osmotic and hypo-osmotic media, but not by other stresses. The expressed proteins of these mRNAs may be involved directly or indirectly in the adaptation of RTG-2 cells to different osmolalities probably through the osmotic signal transduction and adjustment in cellular metabolism to osmotic stress.
Original language | English |
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Pages (from-to) | 255-262 |
Number of pages | 8 |
Journal | Fish Physiology and Biochemistry |
Volume | 22 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2000 |
Externally published | Yes |
Keywords
- CHD3
- Cell culture
- Complement
- Differential mRNA display polymerase chain reaction
- Growth
- Nucleolin
- Protein kinase
- RTG-2 cell
- Rainbow trout
- Salinity
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Aquatic Science