Induction of mRNAs for glutathione synthesis-related proteins in mouse liver by low doses of γ-rays

Shuji Kojima, Osamu Matsuki, Takaharu Nomura, Akiko Kubodera, Yoko Honda, Shuji Honda, Hiroshi Tanooka, Hiro Wakasugi, Kiyonori Yamaoka

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

We examined the elevation of the reduced form of glutathione (GSH) level and the induction of mRNAs for proteins involved in the synthesis and regeneration of GSH in the liver of mice after low-dose γ-ray irradiation. The liver GSH level increased soon after irradiation with 50 cGy of γ-rays, reached a maximum at around 12 h post-treatment. The mRNA of γ-glutamylcysteine synthetase (γ-GCS), the rate-limiting enzyme for de novo synthesis for GSH, showed a small increase that peaked at 6 h after γ-ray irradiation at a dose of 50 cGy. Only a small increase in γ-GCS activity was observed throughout the 24-h post-irradiation period. In the case of glutathione reductase (GR), which is involved in the regeneration of GSH from the oxidized form (GSSG), the mRNA level peaked strongly at 1 h, while the activity peaked at twice the control level 12 h after irradiation. The level of mRNA for thioredoxin (TRX), which contributes to GSH biosynthesis by supplying cysteine to the de novo pathway, peaked at 1 h and declined thereafter, while the activity peaked at 3 h and then declined sharply. These results indicate that the increase in endogenous GSH immediately following low-dose γ-ray irradiation is predominantly due to operation of the regeneration cycle and not de novo synthesis. We also examined the dependence of mRNA induction on the γ-ray dose. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)312-318
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume1381
Issue number3
DOIs
Publication statusPublished - Aug 24 1998
Externally publishedYes

Fingerprint

Liver
Glutathione
Irradiation
Messenger RNA
Regeneration
Dosimetry
Proteins
Glutamate-Cysteine Ligase
Thioredoxins
Glutathione Disulfide
Glutathione Reductase
Biosynthesis
Level control
Cysteine
Enzymes

Keywords

  • Glutathione
  • Glutathione reductase
  • Liver
  • Low dose γ-ray
  • Mouse
  • mRNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Induction of mRNAs for glutathione synthesis-related proteins in mouse liver by low doses of γ-rays. / Kojima, Shuji; Matsuki, Osamu; Nomura, Takaharu; Kubodera, Akiko; Honda, Yoko; Honda, Shuji; Tanooka, Hiroshi; Wakasugi, Hiro; Yamaoka, Kiyonori.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1381, No. 3, 24.08.1998, p. 312-318.

Research output: Contribution to journalArticle

Kojima, Shuji ; Matsuki, Osamu ; Nomura, Takaharu ; Kubodera, Akiko ; Honda, Yoko ; Honda, Shuji ; Tanooka, Hiroshi ; Wakasugi, Hiro ; Yamaoka, Kiyonori. / Induction of mRNAs for glutathione synthesis-related proteins in mouse liver by low doses of γ-rays. In: Biochimica et Biophysica Acta - General Subjects. 1998 ; Vol. 1381, No. 3. pp. 312-318.
@article{abdb2e5206a946b69180c04b80ee88ea,
title = "Induction of mRNAs for glutathione synthesis-related proteins in mouse liver by low doses of γ-rays",
abstract = "We examined the elevation of the reduced form of glutathione (GSH) level and the induction of mRNAs for proteins involved in the synthesis and regeneration of GSH in the liver of mice after low-dose γ-ray irradiation. The liver GSH level increased soon after irradiation with 50 cGy of γ-rays, reached a maximum at around 12 h post-treatment. The mRNA of γ-glutamylcysteine synthetase (γ-GCS), the rate-limiting enzyme for de novo synthesis for GSH, showed a small increase that peaked at 6 h after γ-ray irradiation at a dose of 50 cGy. Only a small increase in γ-GCS activity was observed throughout the 24-h post-irradiation period. In the case of glutathione reductase (GR), which is involved in the regeneration of GSH from the oxidized form (GSSG), the mRNA level peaked strongly at 1 h, while the activity peaked at twice the control level 12 h after irradiation. The level of mRNA for thioredoxin (TRX), which contributes to GSH biosynthesis by supplying cysteine to the de novo pathway, peaked at 1 h and declined thereafter, while the activity peaked at 3 h and then declined sharply. These results indicate that the increase in endogenous GSH immediately following low-dose γ-ray irradiation is predominantly due to operation of the regeneration cycle and not de novo synthesis. We also examined the dependence of mRNA induction on the γ-ray dose. Copyright (C) 1998 Elsevier Science B.V.",
keywords = "Glutathione, Glutathione reductase, Liver, Low dose γ-ray, Mouse, mRNA",
author = "Shuji Kojima and Osamu Matsuki and Takaharu Nomura and Akiko Kubodera and Yoko Honda and Shuji Honda and Hiroshi Tanooka and Hiro Wakasugi and Kiyonori Yamaoka",
year = "1998",
month = "8",
day = "24",
doi = "10.1016/S0304-4165(98)00043-9",
language = "English",
volume = "1381",
pages = "312--318",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Induction of mRNAs for glutathione synthesis-related proteins in mouse liver by low doses of γ-rays

AU - Kojima, Shuji

AU - Matsuki, Osamu

AU - Nomura, Takaharu

AU - Kubodera, Akiko

AU - Honda, Yoko

AU - Honda, Shuji

AU - Tanooka, Hiroshi

AU - Wakasugi, Hiro

AU - Yamaoka, Kiyonori

PY - 1998/8/24

Y1 - 1998/8/24

N2 - We examined the elevation of the reduced form of glutathione (GSH) level and the induction of mRNAs for proteins involved in the synthesis and regeneration of GSH in the liver of mice after low-dose γ-ray irradiation. The liver GSH level increased soon after irradiation with 50 cGy of γ-rays, reached a maximum at around 12 h post-treatment. The mRNA of γ-glutamylcysteine synthetase (γ-GCS), the rate-limiting enzyme for de novo synthesis for GSH, showed a small increase that peaked at 6 h after γ-ray irradiation at a dose of 50 cGy. Only a small increase in γ-GCS activity was observed throughout the 24-h post-irradiation period. In the case of glutathione reductase (GR), which is involved in the regeneration of GSH from the oxidized form (GSSG), the mRNA level peaked strongly at 1 h, while the activity peaked at twice the control level 12 h after irradiation. The level of mRNA for thioredoxin (TRX), which contributes to GSH biosynthesis by supplying cysteine to the de novo pathway, peaked at 1 h and declined thereafter, while the activity peaked at 3 h and then declined sharply. These results indicate that the increase in endogenous GSH immediately following low-dose γ-ray irradiation is predominantly due to operation of the regeneration cycle and not de novo synthesis. We also examined the dependence of mRNA induction on the γ-ray dose. Copyright (C) 1998 Elsevier Science B.V.

AB - We examined the elevation of the reduced form of glutathione (GSH) level and the induction of mRNAs for proteins involved in the synthesis and regeneration of GSH in the liver of mice after low-dose γ-ray irradiation. The liver GSH level increased soon after irradiation with 50 cGy of γ-rays, reached a maximum at around 12 h post-treatment. The mRNA of γ-glutamylcysteine synthetase (γ-GCS), the rate-limiting enzyme for de novo synthesis for GSH, showed a small increase that peaked at 6 h after γ-ray irradiation at a dose of 50 cGy. Only a small increase in γ-GCS activity was observed throughout the 24-h post-irradiation period. In the case of glutathione reductase (GR), which is involved in the regeneration of GSH from the oxidized form (GSSG), the mRNA level peaked strongly at 1 h, while the activity peaked at twice the control level 12 h after irradiation. The level of mRNA for thioredoxin (TRX), which contributes to GSH biosynthesis by supplying cysteine to the de novo pathway, peaked at 1 h and declined thereafter, while the activity peaked at 3 h and then declined sharply. These results indicate that the increase in endogenous GSH immediately following low-dose γ-ray irradiation is predominantly due to operation of the regeneration cycle and not de novo synthesis. We also examined the dependence of mRNA induction on the γ-ray dose. Copyright (C) 1998 Elsevier Science B.V.

KW - Glutathione

KW - Glutathione reductase

KW - Liver

KW - Low dose γ-ray

KW - Mouse

KW - mRNA

UR - http://www.scopus.com/inward/record.url?scp=0031840032&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031840032&partnerID=8YFLogxK

U2 - 10.1016/S0304-4165(98)00043-9

DO - 10.1016/S0304-4165(98)00043-9

M3 - Article

C2 - 9729439

AN - SCOPUS:0031840032

VL - 1381

SP - 312

EP - 318

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0304-4165

IS - 3

ER -