Induction of mRNAs for glutathione synthesis-related proteins in mouse liver by low doses of γ-rays

Shuji Kojima, Osamu Matsuki, Takaharu Nomura, Akiko Kubodera, Yoko Honda, Shuji Honda, Hiroshi Tanooka, Hiro Wakasugi, Kiyonori Yamaoka

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

We examined the elevation of the reduced form of glutathione (GSH) level and the induction of mRNAs for proteins involved in the synthesis and regeneration of GSH in the liver of mice after low-dose γ-ray irradiation. The liver GSH level increased soon after irradiation with 50 cGy of γ-rays, reached a maximum at around 12 h post-treatment. The mRNA of γ-glutamylcysteine synthetase (γ-GCS), the rate-limiting enzyme for de novo synthesis for GSH, showed a small increase that peaked at 6 h after γ-ray irradiation at a dose of 50 cGy. Only a small increase in γ-GCS activity was observed throughout the 24-h post-irradiation period. In the case of glutathione reductase (GR), which is involved in the regeneration of GSH from the oxidized form (GSSG), the mRNA level peaked strongly at 1 h, while the activity peaked at twice the control level 12 h after irradiation. The level of mRNA for thioredoxin (TRX), which contributes to GSH biosynthesis by supplying cysteine to the de novo pathway, peaked at 1 h and declined thereafter, while the activity peaked at 3 h and then declined sharply. These results indicate that the increase in endogenous GSH immediately following low-dose γ-ray irradiation is predominantly due to operation of the regeneration cycle and not de novo synthesis. We also examined the dependence of mRNA induction on the γ-ray dose. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)312-318
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume1381
Issue number3
DOIs
Publication statusPublished - Aug 24 1998
Externally publishedYes

Keywords

  • Glutathione
  • Glutathione reductase
  • Liver
  • Low dose γ-ray
  • Mouse
  • mRNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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