Induction of monocyte chemoattractant protein 1 by heticobacter pylori involves NF-κB

N. Mori, A. Ueda, R. Geleziunas, A. Wada, T. Hirayama, Teizo Yoshimura, N. Yamamoto

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Helicobacter pylori stimulates secretion of monocyle chemoattractant protein 1 (MCP-1) from gastric epithelial cells. Secretion of this chemokine may be instrumental in monocyte infiltration of the gastric epithelium that characterizes H. pylori gastritis. The aim of this study was to identify the mechanism by which H. pylori induces MCP-1 production. Induction of MCP-1 mRNA was assessed by reverse transcription-PCR. We used luciferase reporter assays to monitor activation of the MCP-1 gene promoter and electrophoretic mobility shift assays to explore binding of transcription factors to this promoter. H. pylori infection increased MCP-1 mRNA expression from gastric epithelial cells. Induction of MCP-1 mRNA relies on an intact cag pathogenicity island. We identified two closely spaced NF-κB-binding sites within the MCP-1 distal enhancer as required for H. pylori-induced MCP-1 gene transcription. H. pylori infection led to the specific activation of NF-κB complexes containing p50 and p65. Kinase-deficient mutants of NF-κB-inducing kinase (NIK) and IκB kinases (IKK) caused suppression of MCP-1 distal enhancer-dependent reporter activity following H. pylori infection. H. pylori infection induces the activation of NF-κB via the NIK-IKK signaling complex, leading to MCP-1 gene transcription in gastric epithelial cells.

Original languageEnglish
Pages (from-to)1280-1286
Number of pages7
JournalInfection and Immunity
Volume69
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

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Chemokine CCL2
Pylorus
Chemotactic Factors
Helicobacter pylori
Helicobacter Infections
Proteins
Stomach
Phosphotransferases
Epithelial Cells
Messenger RNA
Genomic Islands
Electrophoretic Mobility Shift Assay
Gastritis
Luciferases
Chemokines
Reverse Transcription
Monocytes
Transcription Factors
Epithelium
Binding Sites

ASJC Scopus subject areas

  • Immunology

Cite this

Induction of monocyte chemoattractant protein 1 by heticobacter pylori involves NF-κB. / Mori, N.; Ueda, A.; Geleziunas, R.; Wada, A.; Hirayama, T.; Yoshimura, Teizo; Yamamoto, N.

In: Infection and Immunity, Vol. 69, No. 3, 2001, p. 1280-1286.

Research output: Contribution to journalArticle

Mori, N. ; Ueda, A. ; Geleziunas, R. ; Wada, A. ; Hirayama, T. ; Yoshimura, Teizo ; Yamamoto, N. / Induction of monocyte chemoattractant protein 1 by heticobacter pylori involves NF-κB. In: Infection and Immunity. 2001 ; Vol. 69, No. 3. pp. 1280-1286.
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