Abstract
Metallothioneins (MTs) are low molecular weight proteins that have been considered to be important metal-binding proteins in the defense against cadmium (Cd) toxicity in animals. These proteins are known to be induced by the injection of heavy metals such as Cd. Previously, we developed the RT-PCR method to measure the level of MT mRNA expression. In this study, we analyzed the time course and dose response of Cd-induced MT-I mRNA expression in the male and female mouse liver. By this method, we measured hepatic MT-I mRNA expression which reached the highest peak 6 h after subcutaneous injection of 1.1 mg/kg Cd in 9-week old male and female mice compared with those of corresponding controls (0 h). There was no statistical difference in the hepatic MT-I mRNA expression between male and female mice 6 h after the injection of this dosage. However, it is notable that the MT-I mRNA expression in male mice was much higher than that in females 6 h after injection of 0.5 mg/kg Cd. Thus, the induction of hepatic MT-I mRNA expression is dependent on the sex of the mouse, the dosage and the time course of Cd.
| Original language | English |
|---|---|
| Pages (from-to) | 663-666 |
| Number of pages | 4 |
| Journal | Methods and Findings in Experimental and Clinical Pharmacology |
| Volume | 22 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2000 |
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Keywords
- Cadmium
- Liver
- Metallothionein
- mRNA expression
- Sex differences
ASJC Scopus subject areas
- Pharmacology (medical)
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Induction of metallothionein mRNA expression in the mouse liver after cadmium injection as measured by the reverse transcriptasepolymerase chain reaction method. / Sogawa, N.; Sogawa, Chiharu; Oda, N.; Fujioka, T.; Onodera, K.; Furuta, H.
In: Methods and Findings in Experimental and Clinical Pharmacology, Vol. 22, No. 9, 2000, p. 663-666.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of metallothionein mRNA expression in the mouse liver after cadmium injection as measured by the reverse transcriptasepolymerase chain reaction method
AU - Sogawa, N.
AU - Sogawa, Chiharu
AU - Oda, N.
AU - Fujioka, T.
AU - Onodera, K.
AU - Furuta, H.
PY - 2000
Y1 - 2000
N2 - Metallothioneins (MTs) are low molecular weight proteins that have been considered to be important metal-binding proteins in the defense against cadmium (Cd) toxicity in animals. These proteins are known to be induced by the injection of heavy metals such as Cd. Previously, we developed the RT-PCR method to measure the level of MT mRNA expression. In this study, we analyzed the time course and dose response of Cd-induced MT-I mRNA expression in the male and female mouse liver. By this method, we measured hepatic MT-I mRNA expression which reached the highest peak 6 h after subcutaneous injection of 1.1 mg/kg Cd in 9-week old male and female mice compared with those of corresponding controls (0 h). There was no statistical difference in the hepatic MT-I mRNA expression between male and female mice 6 h after the injection of this dosage. However, it is notable that the MT-I mRNA expression in male mice was much higher than that in females 6 h after injection of 0.5 mg/kg Cd. Thus, the induction of hepatic MT-I mRNA expression is dependent on the sex of the mouse, the dosage and the time course of Cd.
AB - Metallothioneins (MTs) are low molecular weight proteins that have been considered to be important metal-binding proteins in the defense against cadmium (Cd) toxicity in animals. These proteins are known to be induced by the injection of heavy metals such as Cd. Previously, we developed the RT-PCR method to measure the level of MT mRNA expression. In this study, we analyzed the time course and dose response of Cd-induced MT-I mRNA expression in the male and female mouse liver. By this method, we measured hepatic MT-I mRNA expression which reached the highest peak 6 h after subcutaneous injection of 1.1 mg/kg Cd in 9-week old male and female mice compared with those of corresponding controls (0 h). There was no statistical difference in the hepatic MT-I mRNA expression between male and female mice 6 h after the injection of this dosage. However, it is notable that the MT-I mRNA expression in male mice was much higher than that in females 6 h after injection of 0.5 mg/kg Cd. Thus, the induction of hepatic MT-I mRNA expression is dependent on the sex of the mouse, the dosage and the time course of Cd.
KW - Cadmium
KW - Liver
KW - Metallothionein
KW - mRNA expression
KW - Sex differences
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UR - http://www.scopus.com/inward/citedby.url?scp=0034466945&partnerID=8YFLogxK
U2 - 10.1358/mf.2000.22.9.802280
DO - 10.1358/mf.2000.22.9.802280
M3 - Article
C2 - 11294006
AN - SCOPUS:0034466945
VL - 22
SP - 663
EP - 666
JO - Methods and Findings in Experimental and Clinical Pharmacology
JF - Methods and Findings in Experimental and Clinical Pharmacology
SN - 0379-0355
IS - 9
ER -