Induction of metallothionein mRNA expression in the mouse liver after cadmium injection as measured by the reverse transcriptasepolymerase chain reaction method

N. Sogawa, Chiharu Sogawa, N. Oda, T. Fujioka, K. Onodera, H. Furuta

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Metallothioneins (MTs) are low molecular weight proteins that have been considered to be important metal-binding proteins in the defense against cadmium (Cd) toxicity in animals. These proteins are known to be induced by the injection of heavy metals such as Cd. Previously, we developed the RT-PCR method to measure the level of MT mRNA expression. In this study, we analyzed the time course and dose response of Cd-induced MT-I mRNA expression in the male and female mouse liver. By this method, we measured hepatic MT-I mRNA expression which reached the highest peak 6 h after subcutaneous injection of 1.1 mg/kg Cd in 9-week old male and female mice compared with those of corresponding controls (0 h). There was no statistical difference in the hepatic MT-I mRNA expression between male and female mice 6 h after the injection of this dosage. However, it is notable that the MT-I mRNA expression in male mice was much higher than that in females 6 h after injection of 0.5 mg/kg Cd. Thus, the induction of hepatic MT-I mRNA expression is dependent on the sex of the mouse, the dosage and the time course of Cd.

Original languageEnglish
Pages (from-to)663-666
Number of pages4
JournalMethods and Findings in Experimental and Clinical Pharmacology
Volume22
Issue number9
DOIs
Publication statusPublished - 2000

Fingerprint

Metallothionein
Cadmium
Messenger RNA
Injections
Liver
Subcutaneous Injections
Heavy Metals
Carrier Proteins
Proteins
Molecular Weight
Metals
Polymerase Chain Reaction

Keywords

  • Cadmium
  • Liver
  • Metallothionein
  • mRNA expression
  • Sex differences

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

@article{921cac8d1ff4405b9c0e86fe26118255,
title = "Induction of metallothionein mRNA expression in the mouse liver after cadmium injection as measured by the reverse transcriptasepolymerase chain reaction method",
abstract = "Metallothioneins (MTs) are low molecular weight proteins that have been considered to be important metal-binding proteins in the defense against cadmium (Cd) toxicity in animals. These proteins are known to be induced by the injection of heavy metals such as Cd. Previously, we developed the RT-PCR method to measure the level of MT mRNA expression. In this study, we analyzed the time course and dose response of Cd-induced MT-I mRNA expression in the male and female mouse liver. By this method, we measured hepatic MT-I mRNA expression which reached the highest peak 6 h after subcutaneous injection of 1.1 mg/kg Cd in 9-week old male and female mice compared with those of corresponding controls (0 h). There was no statistical difference in the hepatic MT-I mRNA expression between male and female mice 6 h after the injection of this dosage. However, it is notable that the MT-I mRNA expression in male mice was much higher than that in females 6 h after injection of 0.5 mg/kg Cd. Thus, the induction of hepatic MT-I mRNA expression is dependent on the sex of the mouse, the dosage and the time course of Cd.",
keywords = "Cadmium, Liver, Metallothionein, mRNA expression, Sex differences",
author = "N. Sogawa and Chiharu Sogawa and N. Oda and T. Fujioka and K. Onodera and H. Furuta",
year = "2000",
doi = "10.1358/mf.2000.22.9.802280",
language = "English",
volume = "22",
pages = "663--666",
journal = "Methods and Findings in Experimental and Clinical Pharmacology",
issn = "0379-0355",
publisher = "Prous Science",
number = "9",

}

TY - JOUR

T1 - Induction of metallothionein mRNA expression in the mouse liver after cadmium injection as measured by the reverse transcriptasepolymerase chain reaction method

AU - Sogawa, N.

AU - Sogawa, Chiharu

AU - Oda, N.

AU - Fujioka, T.

AU - Onodera, K.

AU - Furuta, H.

PY - 2000

Y1 - 2000

N2 - Metallothioneins (MTs) are low molecular weight proteins that have been considered to be important metal-binding proteins in the defense against cadmium (Cd) toxicity in animals. These proteins are known to be induced by the injection of heavy metals such as Cd. Previously, we developed the RT-PCR method to measure the level of MT mRNA expression. In this study, we analyzed the time course and dose response of Cd-induced MT-I mRNA expression in the male and female mouse liver. By this method, we measured hepatic MT-I mRNA expression which reached the highest peak 6 h after subcutaneous injection of 1.1 mg/kg Cd in 9-week old male and female mice compared with those of corresponding controls (0 h). There was no statistical difference in the hepatic MT-I mRNA expression between male and female mice 6 h after the injection of this dosage. However, it is notable that the MT-I mRNA expression in male mice was much higher than that in females 6 h after injection of 0.5 mg/kg Cd. Thus, the induction of hepatic MT-I mRNA expression is dependent on the sex of the mouse, the dosage and the time course of Cd.

AB - Metallothioneins (MTs) are low molecular weight proteins that have been considered to be important metal-binding proteins in the defense against cadmium (Cd) toxicity in animals. These proteins are known to be induced by the injection of heavy metals such as Cd. Previously, we developed the RT-PCR method to measure the level of MT mRNA expression. In this study, we analyzed the time course and dose response of Cd-induced MT-I mRNA expression in the male and female mouse liver. By this method, we measured hepatic MT-I mRNA expression which reached the highest peak 6 h after subcutaneous injection of 1.1 mg/kg Cd in 9-week old male and female mice compared with those of corresponding controls (0 h). There was no statistical difference in the hepatic MT-I mRNA expression between male and female mice 6 h after the injection of this dosage. However, it is notable that the MT-I mRNA expression in male mice was much higher than that in females 6 h after injection of 0.5 mg/kg Cd. Thus, the induction of hepatic MT-I mRNA expression is dependent on the sex of the mouse, the dosage and the time course of Cd.

KW - Cadmium

KW - Liver

KW - Metallothionein

KW - mRNA expression

KW - Sex differences

UR - http://www.scopus.com/inward/record.url?scp=0034466945&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034466945&partnerID=8YFLogxK

U2 - 10.1358/mf.2000.22.9.802280

DO - 10.1358/mf.2000.22.9.802280

M3 - Article

C2 - 11294006

AN - SCOPUS:0034466945

VL - 22

SP - 663

EP - 666

JO - Methods and Findings in Experimental and Clinical Pharmacology

JF - Methods and Findings in Experimental and Clinical Pharmacology

SN - 0379-0355

IS - 9

ER -