Induction of leukocyte adherence at the basement membrane zone with subsequent activation of their metabolic pathway by pemphigoid antibodies and complement

K. Iwatsuki, H. Tagami, M. Yamada

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We observed in our in vitro studies that human leukocytes were attached by their complement receptors to the basement membrane zone (BMZ) of the skin specimens pretreated with pemphigoid antibodies and complement. The resultant histologic feature was very similar to that of an early stage of the inflammatory reactions observable in the lesions of bullous pemphigoid. Eosinophils were also attracted to the BMZ in proportion to their number in cell suspensions, but the adhesion of eosinophils occurred neither selectively nor excessively. The nitroblue tetrazolium (NBT) test, which was carried out after the leukocyte attachment test, demonstrated that only those leukocytes bound specifically to the BMZ incorporated NBT dye complexes vigorously to form formazam crystals in their cytoplasms. The present studies showed that complement-fixing anti-BMZ antibodies induce enhancement of leukocyte activities as well as immune adherence to the BMZ, indicating that the enhanced leukocyte function plays a role in the separation between epidermis and dermis in bullous pemphigoid.

Original languageEnglish
Pages (from-to)290-295
Number of pages6
JournalActa Dermato-Venereologica
Volume63
Issue number4
Publication statusPublished - 1983
Externally publishedYes

Fingerprint

Bullous Pemphigoid
Metabolic Networks and Pathways
Basement Membrane
Leukocytes
Antibodies
Nitroblue Tetrazolium
Eosinophils
Complement Receptors
Dermis
Epidermis
Cell Adhesion
Suspensions
Cytoplasm
Coloring Agents
Skin

ASJC Scopus subject areas

  • Dermatology

Cite this

Induction of leukocyte adherence at the basement membrane zone with subsequent activation of their metabolic pathway by pemphigoid antibodies and complement. / Iwatsuki, K.; Tagami, H.; Yamada, M.

In: Acta Dermato-Venereologica, Vol. 63, No. 4, 1983, p. 290-295.

Research output: Contribution to journalArticle

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