Induction of intracellular interleukin-1β signals via type II interleukin-1 receptor in human gingival fibroblasts

H. H. Chou, S. Takashiba, H. Maeda, K. Naruishi, F. Nishimura, H. Arai, H. K. Lu, Y. Murayama

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The type II interleukin-1 receptor (IL-1RII) has been thought to be incapable of transducing signals to cells because of its short intracellular domain, while type I IL-1 receptor (IL-1RI) does transduce signals. Since over-expression of IL-1RII has been demonstrated to inhibit cytokine production in the fibroblastic cell line, it has been proposed to use IL-1RII to prevent IL-1-induced inflammation in connective tissue. In this study, trace amounts of IL-1RII mRNA expression were detected in human gingival fibroblasts (HGFs), which are affected by cytokines in inflammatory periodontal disease. Cloning of the cDNA encoding IL-1RII expressed in HGFs revealed 3 amino acid substitutions in the extracellular domain, when compared with the 408 residues predicted from human B-cells. Over-expression of IL-1RII on HGFs by gene transfer down-regulated the expression of IL-1β mRNA and IL-6 mRNA in response to IL-1β stimulation, while the expression of IL-8 mRNA was not affected. In the IL-1RII-transfected HGFs, phosphorylation of 25- and 74-kDa proteins was up-regulated upon IL-1β stimulation in the transfected HGFs. The phosphorylation of these proteins was suppressed by the addition of a neutralizing antibody against IL-1RII. These results suggest that the IL-1RII may regulate HGFs expression of cytokine mRNA upon IL-1β stimulation, possibly by altering the IL-1RI-dependent signals.

Original languageEnglish
Pages (from-to)1683-1688
Number of pages6
JournalJournal of dental research
Volume79
Issue number9
Publication statusPublished - 2000

Keywords

  • Cytokine gene expression
  • Human gingival fibroblasts
  • Interleukin-1β (IL-1)
  • Signaling
  • Type II IL-1 receptor (IL-1RII)

ASJC Scopus subject areas

  • Dentistry(all)

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