Induction of intracellular interleukin-1β signals via type II interleukin-1 receptor in human gingival fibroblasts

H. H. Chou, Shogo Takashiba, H. Maeda, K. Naruishi, F. Nishimura, H. Arai, H. K. Lu, Y. Murayama

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The type II interleukin-1 receptor (IL-1RII) has been thought to be incapable of transducing signals to cells because of its short intracellular domain, while type I IL-1 receptor (IL-1RI) does transduce signals. Since over-expression of IL-1RII has been demonstrated to inhibit cytokine production in the fibroblastic cell line, it has been proposed to use IL-1RII to prevent IL-1-induced inflammation in connective tissue. In this study, trace amounts of IL-1RII mRNA expression were detected in human gingival fibroblasts (HGFs), which are affected by cytokines in inflammatory periodontal disease. Cloning of the cDNA encoding IL-1RII expressed in HGFs revealed 3 amino acid substitutions in the extracellular domain, when compared with the 408 residues predicted from human B-cells. Over-expression of IL-1RII on HGFs by gene transfer down-regulated the expression of IL-1β mRNA and IL-6 mRNA in response to IL-1β stimulation, while the expression of IL-8 mRNA was not affected. In the IL-1RII-transfected HGFs, phosphorylation of 25- and 74-kDa proteins was up-regulated upon IL-1β stimulation in the transfected HGFs. The phosphorylation of these proteins was suppressed by the addition of a neutralizing antibody against IL-1RII. These results suggest that the IL-1RII may regulate HGFs expression of cytokine mRNA upon IL-1β stimulation, possibly by altering the IL-1RI-dependent signals.

Original languageEnglish
Pages (from-to)1683-1688
Number of pages6
JournalJournal of Dental Research
Volume79
Issue number9
Publication statusPublished - 2000

Fingerprint

Interleukin-1
Fibroblasts
Messenger RNA
Cytokines
Interleukin-1 Type II Receptors
Phosphorylation
Interleukin-1 Receptors
Periodontal Diseases
Amino Acid Substitution
human IL1R2 protein
Neutralizing Antibodies
Interleukin-8
Connective Tissue
Organism Cloning
Interleukin-6
Proteins
B-Lymphocytes
Complementary DNA
Inflammation
Cell Line

Keywords

  • Cytokine gene expression
  • Human gingival fibroblasts
  • Interleukin-1β (IL-1)
  • Signaling
  • Type II IL-1 receptor (IL-1RII)

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Chou, H. H., Takashiba, S., Maeda, H., Naruishi, K., Nishimura, F., Arai, H., ... Murayama, Y. (2000). Induction of intracellular interleukin-1β signals via type II interleukin-1 receptor in human gingival fibroblasts. Journal of Dental Research, 79(9), 1683-1688.

Induction of intracellular interleukin-1β signals via type II interleukin-1 receptor in human gingival fibroblasts. / Chou, H. H.; Takashiba, Shogo; Maeda, H.; Naruishi, K.; Nishimura, F.; Arai, H.; Lu, H. K.; Murayama, Y.

In: Journal of Dental Research, Vol. 79, No. 9, 2000, p. 1683-1688.

Research output: Contribution to journalArticle

Chou, HH, Takashiba, S, Maeda, H, Naruishi, K, Nishimura, F, Arai, H, Lu, HK & Murayama, Y 2000, 'Induction of intracellular interleukin-1β signals via type II interleukin-1 receptor in human gingival fibroblasts', Journal of Dental Research, vol. 79, no. 9, pp. 1683-1688.
Chou, H. H. ; Takashiba, Shogo ; Maeda, H. ; Naruishi, K. ; Nishimura, F. ; Arai, H. ; Lu, H. K. ; Murayama, Y. / Induction of intracellular interleukin-1β signals via type II interleukin-1 receptor in human gingival fibroblasts. In: Journal of Dental Research. 2000 ; Vol. 79, No. 9. pp. 1683-1688.
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