Induction of interdigitating reticulum cell-like differentiation in human monocytic leukemia cells by conditioned medium from IL-2-stimulated helper T-cells

Kiyoshi Takahashi, Katsuya Miyatani, Hiroyuki Yanai, Ho Jong Jeon, Kotaro Fujiwara, Tadashi Yoshino, Kazuhiko Hayashi, Tadaatsu Akagi, Ken Tsutsui, Koichi Mizobuchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Monocytic leukemia (MoL) cells were obtained from the peripheral blood of a patient in whom the leukemic cells infiltrating various lymphoreticular organs exhibited features intermediate between interdigitating reticulum cells (IDC) and ordinary phagocytic macrophages, whereas the leukemic cells in the peripheral blood were essentially monocytic and lacked such features. Peripheral blood CD4+ T-cells were established as an interleukin-2-dependent T-cell line. When the MoL cells were exposed for a few days to conditioned medium from the T-cell line, they extended several dendritic cytoplasmic projections and became intensely positive for HLA-DR antigen, cytoplasmic S-100β protein, and CD1 antigen. Functionally, the conditioned medium significantly down-regulated Fc-mediated and Fc-independent phagocytic activities, and the levels of lysosomal enzymes such as lysozyme and nonspecific esterase in the MoL cells. Moreover, the conditioned medium significantly up-regulated the accessory cell function of the MoL cells as measured by the primary allogenic mixed leukocyte reaction (MLR). Furthermore, the conditioned medium significantly down-regulated the expression of CD14 antigen. Biochemical analysis indicated that the factor responsible for these changes is a protein which is distinct from known human cytokines and whose molecular weight is approximately 31 kDa. These findings suggest that IDC are closely related the monocytic lineage and that helper T-cells play an important role in constructing the microenvironment of T-lymphoid tissues which is necessary for the differentiation and maturation of IDC.

Original languageEnglish
Pages (from-to)105-113
Number of pages9
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Volume62
Issue number1
DOIs
Publication statusPublished - Dec 1992

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Reticulum
Conditioned Culture Medium
Helper-Inducer T-Lymphocytes
Dendritic Cells
Interleukin-2
Cell Differentiation
Leukemia
S100 Proteins
T-Lymphocytes
CD1 Antigens
CD14 Antigens
Cell Line
Carboxylesterase
Mixed Lymphocyte Culture Test
Lymphoid Tissue
HLA-DR Antigens
Muramidase
Statistical Factor Analysis
Molecular Weight
Macrophages

Keywords

  • Cytokine
  • Differentiation
  • Helper T-cells
  • Interdigitating reticulum cells
  • Monocytic leukemia cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Induction of interdigitating reticulum cell-like differentiation in human monocytic leukemia cells by conditioned medium from IL-2-stimulated helper T-cells. / Takahashi, Kiyoshi; Miyatani, Katsuya; Yanai, Hiroyuki; Jeon, Ho Jong; Fujiwara, Kotaro; Yoshino, Tadashi; Hayashi, Kazuhiko; Akagi, Tadaatsu; Tsutsui, Ken; Mizobuchi, Koichi.

In: Virchows Archiv B Cell Pathology Including Molecular Pathology, Vol. 62, No. 1, 12.1992, p. 105-113.

Research output: Contribution to journalArticle

Takahashi, Kiyoshi ; Miyatani, Katsuya ; Yanai, Hiroyuki ; Jeon, Ho Jong ; Fujiwara, Kotaro ; Yoshino, Tadashi ; Hayashi, Kazuhiko ; Akagi, Tadaatsu ; Tsutsui, Ken ; Mizobuchi, Koichi. / Induction of interdigitating reticulum cell-like differentiation in human monocytic leukemia cells by conditioned medium from IL-2-stimulated helper T-cells. In: Virchows Archiv B Cell Pathology Including Molecular Pathology. 1992 ; Vol. 62, No. 1. pp. 105-113.
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