Induction of heat shock protein genes by chlorfenapyr in cultured cells of the cabbage armyworm, Mamestra brassicae

S. Sonoda, H. Tsumuki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Effects of structurally different insecticides, permethrin, chlorfluazuron, chlorfenapyr, prothiofos, methomyl, and thiocyclam on expression of hsp90, hsp70, hsp20.7, and hsp19.7 were examined using cultured cells of the cabbage armyworm, Mamestra brassicae. Significant induction of hsp90, hsp70, hsp20.7, and hsp19.7 expression was observed in response to chlorfenapyr. No induction was observed for the remaining five insecticides. The chlorfenapyr-induced expression was time-dependent and concentration-dependent. A significant reduction in expression levels of hsp70, hsp20.7, and hsp19.7 was observed in the time course when the cells exposed to chlorfenapyr were transferred to chlorfenapyr-free medium. These results suggest that hsp70, hsp20.7, and hsp19.7 might be useful to assess cellular distress or injury by chlorfenapyr.

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalPesticide Biochemistry and Physiology
Volume89
Issue number3
DOIs
Publication statusPublished - Nov 2007

Fingerprint

chlorfenapyr
Mamestra brassicae
Brassica
Heat-Shock Proteins
heat shock proteins
cabbage
cultured cells
Cultured Cells
Genes
Cells
genes
Insecticides
Methomyl
insecticides
chlorfluazuron
Permethrin
methomyl
permethrin
distress
Wounds and Injuries

Keywords

  • Biomarker
  • Cabbage armyworm
  • Chlorfenapyr
  • Heat shock protein
  • Insecticide
  • Mamestra brassicae

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Biochemistry
  • Physiology

Cite this

Induction of heat shock protein genes by chlorfenapyr in cultured cells of the cabbage armyworm, Mamestra brassicae. / Sonoda, S.; Tsumuki, H.

In: Pesticide Biochemistry and Physiology, Vol. 89, No. 3, 11.2007, p. 185-189.

Research output: Contribution to journalArticle

@article{d26462e2c15b40b89bdd500af4b95240,
title = "Induction of heat shock protein genes by chlorfenapyr in cultured cells of the cabbage armyworm, Mamestra brassicae",
abstract = "Effects of structurally different insecticides, permethrin, chlorfluazuron, chlorfenapyr, prothiofos, methomyl, and thiocyclam on expression of hsp90, hsp70, hsp20.7, and hsp19.7 were examined using cultured cells of the cabbage armyworm, Mamestra brassicae. Significant induction of hsp90, hsp70, hsp20.7, and hsp19.7 expression was observed in response to chlorfenapyr. No induction was observed for the remaining five insecticides. The chlorfenapyr-induced expression was time-dependent and concentration-dependent. A significant reduction in expression levels of hsp70, hsp20.7, and hsp19.7 was observed in the time course when the cells exposed to chlorfenapyr were transferred to chlorfenapyr-free medium. These results suggest that hsp70, hsp20.7, and hsp19.7 might be useful to assess cellular distress or injury by chlorfenapyr.",
keywords = "Biomarker, Cabbage armyworm, Chlorfenapyr, Heat shock protein, Insecticide, Mamestra brassicae",
author = "S. Sonoda and H. Tsumuki",
year = "2007",
month = "11",
doi = "10.1016/j.pestbp.2007.06.003",
language = "English",
volume = "89",
pages = "185--189",
journal = "Pesticide Biochemistry and Physiology",
issn = "0048-3575",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Induction of heat shock protein genes by chlorfenapyr in cultured cells of the cabbage armyworm, Mamestra brassicae

AU - Sonoda, S.

AU - Tsumuki, H.

PY - 2007/11

Y1 - 2007/11

N2 - Effects of structurally different insecticides, permethrin, chlorfluazuron, chlorfenapyr, prothiofos, methomyl, and thiocyclam on expression of hsp90, hsp70, hsp20.7, and hsp19.7 were examined using cultured cells of the cabbage armyworm, Mamestra brassicae. Significant induction of hsp90, hsp70, hsp20.7, and hsp19.7 expression was observed in response to chlorfenapyr. No induction was observed for the remaining five insecticides. The chlorfenapyr-induced expression was time-dependent and concentration-dependent. A significant reduction in expression levels of hsp70, hsp20.7, and hsp19.7 was observed in the time course when the cells exposed to chlorfenapyr were transferred to chlorfenapyr-free medium. These results suggest that hsp70, hsp20.7, and hsp19.7 might be useful to assess cellular distress or injury by chlorfenapyr.

AB - Effects of structurally different insecticides, permethrin, chlorfluazuron, chlorfenapyr, prothiofos, methomyl, and thiocyclam on expression of hsp90, hsp70, hsp20.7, and hsp19.7 were examined using cultured cells of the cabbage armyworm, Mamestra brassicae. Significant induction of hsp90, hsp70, hsp20.7, and hsp19.7 expression was observed in response to chlorfenapyr. No induction was observed for the remaining five insecticides. The chlorfenapyr-induced expression was time-dependent and concentration-dependent. A significant reduction in expression levels of hsp70, hsp20.7, and hsp19.7 was observed in the time course when the cells exposed to chlorfenapyr were transferred to chlorfenapyr-free medium. These results suggest that hsp70, hsp20.7, and hsp19.7 might be useful to assess cellular distress or injury by chlorfenapyr.

KW - Biomarker

KW - Cabbage armyworm

KW - Chlorfenapyr

KW - Heat shock protein

KW - Insecticide

KW - Mamestra brassicae

UR - http://www.scopus.com/inward/record.url?scp=34548784312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548784312&partnerID=8YFLogxK

U2 - 10.1016/j.pestbp.2007.06.003

DO - 10.1016/j.pestbp.2007.06.003

M3 - Article

AN - SCOPUS:34548784312

VL - 89

SP - 185

EP - 189

JO - Pesticide Biochemistry and Physiology

JF - Pesticide Biochemistry and Physiology

SN - 0048-3575

IS - 3

ER -