Induction of heat shock protein genes by chlorfenapyr in cultured cells of the cabbage armyworm, Mamestra brassicae

S. Sonoda; H. Tsumuki

doi:10.1016/j.pestbp.2007.06.003

Induction of heat shock protein genes by chlorfenapyr in cultured cells of the cabbage armyworm, Mamestra brassicae

S. Sonoda, H. Tsumuki

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Effects of structurally different insecticides, permethrin, chlorfluazuron, chlorfenapyr, prothiofos, methomyl, and thiocyclam on expression of hsp90, hsp70, hsp20.7, and hsp19.7 were examined using cultured cells of the cabbage armyworm, Mamestra brassicae. Significant induction of hsp90, hsp70, hsp20.7, and hsp19.7 expression was observed in response to chlorfenapyr. No induction was observed for the remaining five insecticides. The chlorfenapyr-induced expression was time-dependent and concentration-dependent. A significant reduction in expression levels of hsp70, hsp20.7, and hsp19.7 was observed in the time course when the cells exposed to chlorfenapyr were transferred to chlorfenapyr-free medium. These results suggest that hsp70, hsp20.7, and hsp19.7 might be useful to assess cellular distress or injury by chlorfenapyr.

Original language	English
Pages (from-to)	185-189
Number of pages	5
Journal	Pesticide Biochemistry and Physiology
Volume	89
Issue number	3
DOIs	https://doi.org/10.1016/j.pestbp.2007.06.003
Publication status	Published - Nov 1 2007

Keywords

Biomarker
Cabbage armyworm
Chlorfenapyr
Heat shock protein
Insecticide
Mamestra brassicae

ASJC Scopus subject areas

Agronomy and Crop Science
Health, Toxicology and Mutagenesis

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title = "Induction of heat shock protein genes by chlorfenapyr in cultured cells of the cabbage armyworm, Mamestra brassicae",

abstract = "Effects of structurally different insecticides, permethrin, chlorfluazuron, chlorfenapyr, prothiofos, methomyl, and thiocyclam on expression of hsp90, hsp70, hsp20.7, and hsp19.7 were examined using cultured cells of the cabbage armyworm, Mamestra brassicae. Significant induction of hsp90, hsp70, hsp20.7, and hsp19.7 expression was observed in response to chlorfenapyr. No induction was observed for the remaining five insecticides. The chlorfenapyr-induced expression was time-dependent and concentration-dependent. A significant reduction in expression levels of hsp70, hsp20.7, and hsp19.7 was observed in the time course when the cells exposed to chlorfenapyr were transferred to chlorfenapyr-free medium. These results suggest that hsp70, hsp20.7, and hsp19.7 might be useful to assess cellular distress or injury by chlorfenapyr.",

keywords = "Biomarker, Cabbage armyworm, Chlorfenapyr, Heat shock protein, Insecticide, Mamestra brassicae",

author = "S. Sonoda and H. Tsumuki",

year = "2007",

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doi = "10.1016/j.pestbp.2007.06.003",

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AU - Sonoda, S.

AU - Tsumuki, H.

PY - 2007/11/1

Y1 - 2007/11/1

N2 - Effects of structurally different insecticides, permethrin, chlorfluazuron, chlorfenapyr, prothiofos, methomyl, and thiocyclam on expression of hsp90, hsp70, hsp20.7, and hsp19.7 were examined using cultured cells of the cabbage armyworm, Mamestra brassicae. Significant induction of hsp90, hsp70, hsp20.7, and hsp19.7 expression was observed in response to chlorfenapyr. No induction was observed for the remaining five insecticides. The chlorfenapyr-induced expression was time-dependent and concentration-dependent. A significant reduction in expression levels of hsp70, hsp20.7, and hsp19.7 was observed in the time course when the cells exposed to chlorfenapyr were transferred to chlorfenapyr-free medium. These results suggest that hsp70, hsp20.7, and hsp19.7 might be useful to assess cellular distress or injury by chlorfenapyr.

AB - Effects of structurally different insecticides, permethrin, chlorfluazuron, chlorfenapyr, prothiofos, methomyl, and thiocyclam on expression of hsp90, hsp70, hsp20.7, and hsp19.7 were examined using cultured cells of the cabbage armyworm, Mamestra brassicae. Significant induction of hsp90, hsp70, hsp20.7, and hsp19.7 expression was observed in response to chlorfenapyr. No induction was observed for the remaining five insecticides. The chlorfenapyr-induced expression was time-dependent and concentration-dependent. A significant reduction in expression levels of hsp70, hsp20.7, and hsp19.7 was observed in the time course when the cells exposed to chlorfenapyr were transferred to chlorfenapyr-free medium. These results suggest that hsp70, hsp20.7, and hsp19.7 might be useful to assess cellular distress or injury by chlorfenapyr.

KW - Biomarker

KW - Cabbage armyworm

KW - Chlorfenapyr

KW - Heat shock protein

KW - Insecticide

KW - Mamestra brassicae

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