Induction of estrus in pubertal miniature gilts

Takuya Wakai, Hozumi Tanaka, Ken ichi Yamanaka, Satoshi Sugimura, Hiroshi Sasada, Manabu Kawahara, Eiji Kobayashi, Eimei Sato

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Genetic engineering of miniature pigs has facilitated the development of numerous biomedical applications, such as xenotransplantation and animal models for human diseases. Manipulation of the estrus is one of the essential techniques for the generation of transgenic offspring. The purpose of the present study was to establish a useful method for induction of the estrus in miniature gilts. A total of 38 pubertal miniature gilts derived from 4 different strains were treated with exogenous gonadotropins. Estrus and ovulatory response were examined after treatment with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) as 200 IU PMSG and 100 IU hCG, 300 IU PMSG and 150 IU hCG, or 1500 IU PMSG only, followed by 100, 150 or 750 IU hCG 72 h later, respectively. The optimal protocol was determined to be the combination treatment of 200 IU PMSG and 100 IU hCG followed by 100 IU hCG. The administration of 200 IU PMSG and 100 IU hCG was effective in inducing estrus regardless of the strain, although there was a strain difference in the ovulatory response. These results indicate that treatment with a low-dose combination of PMSG and hCG provides one of the simplest methods for induction of estrus and ovulation in pubertal miniature pigs.

Original languageEnglish
Pages (from-to)193-198
Number of pages6
JournalAnimal Reproduction Science
Volume103
Issue number1-2
DOIs
Publication statusPublished - Jan 15 2008
Externally publishedYes

Fingerprint

Equine Gonadotropins
pregnant mare serum gonadotropin
human chorionic gonadotropin
Estrus
Chorionic Gonadotropin
gilts
estrus
miniature swine
Swine
xenotransplantation
Animal Disease Models
Heterologous Transplantation
Genetic Engineering
Ovulation Induction
strain differences
gonadotropins
Gonadotropins
genetic engineering
human diseases
ovulation

Keywords

  • Estrus
  • Gonadotropin
  • Miniature pigs
  • Puberty

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biotechnology
  • Genetics

Cite this

Wakai, T., Tanaka, H., Yamanaka, K. I., Sugimura, S., Sasada, H., Kawahara, M., ... Sato, E. (2008). Induction of estrus in pubertal miniature gilts. Animal Reproduction Science, 103(1-2), 193-198. https://doi.org/10.1016/j.anireprosci.2007.04.004

Induction of estrus in pubertal miniature gilts. / Wakai, Takuya; Tanaka, Hozumi; Yamanaka, Ken ichi; Sugimura, Satoshi; Sasada, Hiroshi; Kawahara, Manabu; Kobayashi, Eiji; Sato, Eimei.

In: Animal Reproduction Science, Vol. 103, No. 1-2, 15.01.2008, p. 193-198.

Research output: Contribution to journalArticle

Wakai, T, Tanaka, H, Yamanaka, KI, Sugimura, S, Sasada, H, Kawahara, M, Kobayashi, E & Sato, E 2008, 'Induction of estrus in pubertal miniature gilts', Animal Reproduction Science, vol. 103, no. 1-2, pp. 193-198. https://doi.org/10.1016/j.anireprosci.2007.04.004
Wakai T, Tanaka H, Yamanaka KI, Sugimura S, Sasada H, Kawahara M et al. Induction of estrus in pubertal miniature gilts. Animal Reproduction Science. 2008 Jan 15;103(1-2):193-198. https://doi.org/10.1016/j.anireprosci.2007.04.004
Wakai, Takuya ; Tanaka, Hozumi ; Yamanaka, Ken ichi ; Sugimura, Satoshi ; Sasada, Hiroshi ; Kawahara, Manabu ; Kobayashi, Eiji ; Sato, Eimei. / Induction of estrus in pubertal miniature gilts. In: Animal Reproduction Science. 2008 ; Vol. 103, No. 1-2. pp. 193-198.
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