Induction of donor-specific hyporesponsiveness and prolongation of cardiac allograft survival by jejunal administration of donor splenocytes

Nobuhiro Ishido, Junji Matsuoka, Tsuyoshi Matsuno, Kazuhiko Nakagawa, Noriaki Tanaka

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Abstract

Background. Donor-specific immunosuppression is important in transplantation surgery. We examined the immunosuppressive effects of donor splenocytes administered postoperatively into the jejunum and the effect of such treatment on the survival of heterotopic vascularized cardiac allograft in rats. Methods. Lewis (LEW, RT-11) recipient rats were treated with 5 x 107 Brown Norway (BN, RT-1(n)) donor splenocytes for 5 days orally, intrajejunally, or subcutaneously. The immune responses of LEW treated with either donor BN or irrelevant Wistar King A (WKA, RT-1(k)) were examined by mixed lymphocyte reaction (MLR) and delayed type hypersensitivity (DTH). The effect of postoperative enteral treatment for 6 days with suboptimal dose of cyclosporine (CsA) on heterotopic cardiac allotransplantation was investigated. We measured the production of cytokines (interleukin [IL]-2, IL-4, IL-10, and interferon-γ [IFN-γ]) in the supernatant of MLR by ELISA. The effect of intravenous dose of GdCl3 to block Kupffer cell function was also investigated before the administration of splenocytes. Results. MLR and DTH responses were strongly inhibited in a BN-restricted manner after jejunal or oral feeding of donor BN splenocytes but not by subcutaneous injection or injections by any routs of WKA splenocytes. The effect was more prominent in jejunal than oral feeding. Immunosuppression was associated with a significant inhibition of IL-2 and IFN-γ production and increased concentrations of IL-4 and IL-10 in MLR supernatants. Immunosuppression was abrogated by pretreatment with GdCl3. Postoperative intrajejunal feeding of donor splenocytes with CsA significantly prolonged cardiac allograft survival time (18.7±7.3 vs. 9.9±1.7 days for control animals). Conclusion. Jejunal administration of splenocytes produces donor-specific immunosuppression and prolongs cardiac allograft survival. Our results suggest the involvement of T helper (Th) 2 cytokines and Kupffer cells in the induction of immune hyporesponsiveness, and indicate that this method represents a unique approach for induction of donor-specific immunosuppression.

Original languageEnglish
Pages (from-to)1377-1382
Number of pages6
JournalTransplantation
Volume68
Issue number9
Publication statusPublished - Nov 15 1999

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Mixed Lymphocyte Culture Test
Immunosuppression
Allografts
Kupffer Cells
Delayed Hypersensitivity
Interleukin-4
Interleukin-10
Interferons
Interleukin-2
Cytokines
Th2 Cells
Jejunum
Subcutaneous Injections
Norway
Immunosuppressive Agents
Cyclosporine
Small Intestine
Transplantation
Enzyme-Linked Immunosorbent Assay
Injections

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Induction of donor-specific hyporesponsiveness and prolongation of cardiac allograft survival by jejunal administration of donor splenocytes. / Ishido, Nobuhiro; Matsuoka, Junji; Matsuno, Tsuyoshi; Nakagawa, Kazuhiko; Tanaka, Noriaki.

In: Transplantation, Vol. 68, No. 9, 15.11.1999, p. 1377-1382.

Research output: Contribution to journalArticle

Ishido, Nobuhiro ; Matsuoka, Junji ; Matsuno, Tsuyoshi ; Nakagawa, Kazuhiko ; Tanaka, Noriaki. / Induction of donor-specific hyporesponsiveness and prolongation of cardiac allograft survival by jejunal administration of donor splenocytes. In: Transplantation. 1999 ; Vol. 68, No. 9. pp. 1377-1382.
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abstract = "Background. Donor-specific immunosuppression is important in transplantation surgery. We examined the immunosuppressive effects of donor splenocytes administered postoperatively into the jejunum and the effect of such treatment on the survival of heterotopic vascularized cardiac allograft in rats. Methods. Lewis (LEW, RT-11) recipient rats were treated with 5 x 107 Brown Norway (BN, RT-1(n)) donor splenocytes for 5 days orally, intrajejunally, or subcutaneously. The immune responses of LEW treated with either donor BN or irrelevant Wistar King A (WKA, RT-1(k)) were examined by mixed lymphocyte reaction (MLR) and delayed type hypersensitivity (DTH). The effect of postoperative enteral treatment for 6 days with suboptimal dose of cyclosporine (CsA) on heterotopic cardiac allotransplantation was investigated. We measured the production of cytokines (interleukin [IL]-2, IL-4, IL-10, and interferon-γ [IFN-γ]) in the supernatant of MLR by ELISA. The effect of intravenous dose of GdCl3 to block Kupffer cell function was also investigated before the administration of splenocytes. Results. MLR and DTH responses were strongly inhibited in a BN-restricted manner after jejunal or oral feeding of donor BN splenocytes but not by subcutaneous injection or injections by any routs of WKA splenocytes. The effect was more prominent in jejunal than oral feeding. Immunosuppression was associated with a significant inhibition of IL-2 and IFN-γ production and increased concentrations of IL-4 and IL-10 in MLR supernatants. Immunosuppression was abrogated by pretreatment with GdCl3. Postoperative intrajejunal feeding of donor splenocytes with CsA significantly prolonged cardiac allograft survival time (18.7±7.3 vs. 9.9±1.7 days for control animals). Conclusion. Jejunal administration of splenocytes produces donor-specific immunosuppression and prolongs cardiac allograft survival. Our results suggest the involvement of T helper (Th) 2 cytokines and Kupffer cells in the induction of immune hyporesponsiveness, and indicate that this method represents a unique approach for induction of donor-specific immunosuppression.",
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T1 - Induction of donor-specific hyporesponsiveness and prolongation of cardiac allograft survival by jejunal administration of donor splenocytes

AU - Ishido, Nobuhiro

AU - Matsuoka, Junji

AU - Matsuno, Tsuyoshi

AU - Nakagawa, Kazuhiko

AU - Tanaka, Noriaki

PY - 1999/11/15

Y1 - 1999/11/15

N2 - Background. Donor-specific immunosuppression is important in transplantation surgery. We examined the immunosuppressive effects of donor splenocytes administered postoperatively into the jejunum and the effect of such treatment on the survival of heterotopic vascularized cardiac allograft in rats. Methods. Lewis (LEW, RT-11) recipient rats were treated with 5 x 107 Brown Norway (BN, RT-1(n)) donor splenocytes for 5 days orally, intrajejunally, or subcutaneously. The immune responses of LEW treated with either donor BN or irrelevant Wistar King A (WKA, RT-1(k)) were examined by mixed lymphocyte reaction (MLR) and delayed type hypersensitivity (DTH). The effect of postoperative enteral treatment for 6 days with suboptimal dose of cyclosporine (CsA) on heterotopic cardiac allotransplantation was investigated. We measured the production of cytokines (interleukin [IL]-2, IL-4, IL-10, and interferon-γ [IFN-γ]) in the supernatant of MLR by ELISA. The effect of intravenous dose of GdCl3 to block Kupffer cell function was also investigated before the administration of splenocytes. Results. MLR and DTH responses were strongly inhibited in a BN-restricted manner after jejunal or oral feeding of donor BN splenocytes but not by subcutaneous injection or injections by any routs of WKA splenocytes. The effect was more prominent in jejunal than oral feeding. Immunosuppression was associated with a significant inhibition of IL-2 and IFN-γ production and increased concentrations of IL-4 and IL-10 in MLR supernatants. Immunosuppression was abrogated by pretreatment with GdCl3. Postoperative intrajejunal feeding of donor splenocytes with CsA significantly prolonged cardiac allograft survival time (18.7±7.3 vs. 9.9±1.7 days for control animals). Conclusion. Jejunal administration of splenocytes produces donor-specific immunosuppression and prolongs cardiac allograft survival. Our results suggest the involvement of T helper (Th) 2 cytokines and Kupffer cells in the induction of immune hyporesponsiveness, and indicate that this method represents a unique approach for induction of donor-specific immunosuppression.

AB - Background. Donor-specific immunosuppression is important in transplantation surgery. We examined the immunosuppressive effects of donor splenocytes administered postoperatively into the jejunum and the effect of such treatment on the survival of heterotopic vascularized cardiac allograft in rats. Methods. Lewis (LEW, RT-11) recipient rats were treated with 5 x 107 Brown Norway (BN, RT-1(n)) donor splenocytes for 5 days orally, intrajejunally, or subcutaneously. The immune responses of LEW treated with either donor BN or irrelevant Wistar King A (WKA, RT-1(k)) were examined by mixed lymphocyte reaction (MLR) and delayed type hypersensitivity (DTH). The effect of postoperative enteral treatment for 6 days with suboptimal dose of cyclosporine (CsA) on heterotopic cardiac allotransplantation was investigated. We measured the production of cytokines (interleukin [IL]-2, IL-4, IL-10, and interferon-γ [IFN-γ]) in the supernatant of MLR by ELISA. The effect of intravenous dose of GdCl3 to block Kupffer cell function was also investigated before the administration of splenocytes. Results. MLR and DTH responses were strongly inhibited in a BN-restricted manner after jejunal or oral feeding of donor BN splenocytes but not by subcutaneous injection or injections by any routs of WKA splenocytes. The effect was more prominent in jejunal than oral feeding. Immunosuppression was associated with a significant inhibition of IL-2 and IFN-γ production and increased concentrations of IL-4 and IL-10 in MLR supernatants. Immunosuppression was abrogated by pretreatment with GdCl3. Postoperative intrajejunal feeding of donor splenocytes with CsA significantly prolonged cardiac allograft survival time (18.7±7.3 vs. 9.9±1.7 days for control animals). Conclusion. Jejunal administration of splenocytes produces donor-specific immunosuppression and prolongs cardiac allograft survival. Our results suggest the involvement of T helper (Th) 2 cytokines and Kupffer cells in the induction of immune hyporesponsiveness, and indicate that this method represents a unique approach for induction of donor-specific immunosuppression.

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