Abstract
A variety of tannin and lignin-related compounds were compared for their ability to induce nucleosome-sized DNA fragmentation (a biochemical hallmark of apoptosis), using agarose gel electrophoresis and a fluorescence activated cell sorter. Monomeric, dimeric, trimeric and tetrameric hydrolysable tannins induced nucleosome-sized DNA fragmentation in HL-60 cells, more potently than condensed tannins. The highest activity was detected in gallic acid, a component unit of tannins. Natural lignified materials, except for caffeic acid and its dehydrogenation polymer, showed much weaker activity. Protein-bound polysaccharide (PSK) was inactive. Gallic acid induced DNA fragmentation in four human myelogenous leukaemic cell lines, but not in human T-cell leukaemia and erythroleukaemia cell lines. Ca2+ depletion from the culture medium slightly, but significantly, reduced the apoptosis-inducing activity of gallic acid, but did not significantly affect that of tannic acid or caffeic acid. After treatment with gallic acid, intracellular Ca2+ concentration was significantly elevated. The apoptosis-inducing activity of polyphenols may further emphasize their medicinal efficacy.
| Original language | English |
|---|---|
| Pages (from-to) | 2121-2128 |
| Number of pages | 8 |
| Journal | Anticancer Research |
| Volume | 15 |
| Issue number | 5 B |
| Publication status | Published - 1995 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Calcium
- DNA fragmentation
- Lignin
- Tannin
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Induction of DNA fragmentation by tannin- and lignin-related substances. / Sakagami, H.; Kuribayashi, N.; Iida, M.; Sakagami, T.; Takeda, M.; Fukuchi, K.; Gomi, K.; Ohata, H.; Momose, K.; Kawazoe, Y.; Hatano, Tsutomu; Yoshida, T.; Okuda, T.
In: Anticancer Research, Vol. 15, No. 5 B, 1995, p. 2121-2128.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of DNA fragmentation by tannin- and lignin-related substances
AU - Sakagami, H.
AU - Kuribayashi, N.
AU - Iida, M.
AU - Sakagami, T.
AU - Takeda, M.
AU - Fukuchi, K.
AU - Gomi, K.
AU - Ohata, H.
AU - Momose, K.
AU - Kawazoe, Y.
AU - Hatano, Tsutomu
AU - Yoshida, T.
AU - Okuda, T.
PY - 1995
Y1 - 1995
N2 - A variety of tannin and lignin-related compounds were compared for their ability to induce nucleosome-sized DNA fragmentation (a biochemical hallmark of apoptosis), using agarose gel electrophoresis and a fluorescence activated cell sorter. Monomeric, dimeric, trimeric and tetrameric hydrolysable tannins induced nucleosome-sized DNA fragmentation in HL-60 cells, more potently than condensed tannins. The highest activity was detected in gallic acid, a component unit of tannins. Natural lignified materials, except for caffeic acid and its dehydrogenation polymer, showed much weaker activity. Protein-bound polysaccharide (PSK) was inactive. Gallic acid induced DNA fragmentation in four human myelogenous leukaemic cell lines, but not in human T-cell leukaemia and erythroleukaemia cell lines. Ca2+ depletion from the culture medium slightly, but significantly, reduced the apoptosis-inducing activity of gallic acid, but did not significantly affect that of tannic acid or caffeic acid. After treatment with gallic acid, intracellular Ca2+ concentration was significantly elevated. The apoptosis-inducing activity of polyphenols may further emphasize their medicinal efficacy.
AB - A variety of tannin and lignin-related compounds were compared for their ability to induce nucleosome-sized DNA fragmentation (a biochemical hallmark of apoptosis), using agarose gel electrophoresis and a fluorescence activated cell sorter. Monomeric, dimeric, trimeric and tetrameric hydrolysable tannins induced nucleosome-sized DNA fragmentation in HL-60 cells, more potently than condensed tannins. The highest activity was detected in gallic acid, a component unit of tannins. Natural lignified materials, except for caffeic acid and its dehydrogenation polymer, showed much weaker activity. Protein-bound polysaccharide (PSK) was inactive. Gallic acid induced DNA fragmentation in four human myelogenous leukaemic cell lines, but not in human T-cell leukaemia and erythroleukaemia cell lines. Ca2+ depletion from the culture medium slightly, but significantly, reduced the apoptosis-inducing activity of gallic acid, but did not significantly affect that of tannic acid or caffeic acid. After treatment with gallic acid, intracellular Ca2+ concentration was significantly elevated. The apoptosis-inducing activity of polyphenols may further emphasize their medicinal efficacy.
KW - Apoptosis
KW - Calcium
KW - DNA fragmentation
KW - Lignin
KW - Tannin
UR - http://www.scopus.com/inward/record.url?scp=0029591280&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029591280&partnerID=8YFLogxK
M3 - Article
VL - 15
SP - 2121
EP - 2128
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 5 B
ER -