Induction of DNA fragmentation by tannin- and lignin-related substances

H. Sakagami, N. Kuribayashi, M. Iida, T. Sakagami, M. Takeda, K. Fukuchi, K. Gomi, H. Ohata, K. Momose, Y. Kawazoe, Tsutomu Hatano, T. Yoshida, T. Okuda

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

A variety of tannin and lignin-related compounds were compared for their ability to induce nucleosome-sized DNA fragmentation (a biochemical hallmark of apoptosis), using agarose gel electrophoresis and a fluorescence activated cell sorter. Monomeric, dimeric, trimeric and tetrameric hydrolysable tannins induced nucleosome-sized DNA fragmentation in HL-60 cells, more potently than condensed tannins. The highest activity was detected in gallic acid, a component unit of tannins. Natural lignified materials, except for caffeic acid and its dehydrogenation polymer, showed much weaker activity. Protein-bound polysaccharide (PSK) was inactive. Gallic acid induced DNA fragmentation in four human myelogenous leukaemic cell lines, but not in human T-cell leukaemia and erythroleukaemia cell lines. Ca2+ depletion from the culture medium slightly, but significantly, reduced the apoptosis-inducing activity of gallic acid, but did not significantly affect that of tannic acid or caffeic acid. After treatment with gallic acid, intracellular Ca2+ concentration was significantly elevated. The apoptosis-inducing activity of polyphenols may further emphasize their medicinal efficacy.

Original languageEnglish
Pages (from-to)2121-2128
Number of pages8
JournalAnticancer Research
Volume15
Issue number5 B
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Gallic Acid
Lignin
Tannins
DNA Fragmentation
Nucleosomes
Apoptosis
Hydrolyzable Tannins
T-Cell Leukemia
Cell Line
Proanthocyanidins
Leukemia, Erythroblastic, Acute
Agar Gel Electrophoresis
HL-60 Cells
Polyphenols
Polysaccharides
Culture Media
Polymers
Fluorescence
Proteins
caffeic acid

Keywords

  • Apoptosis
  • Calcium
  • DNA fragmentation
  • Lignin
  • Tannin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sakagami, H., Kuribayashi, N., Iida, M., Sakagami, T., Takeda, M., Fukuchi, K., ... Okuda, T. (1995). Induction of DNA fragmentation by tannin- and lignin-related substances. Anticancer Research, 15(5 B), 2121-2128.

Induction of DNA fragmentation by tannin- and lignin-related substances. / Sakagami, H.; Kuribayashi, N.; Iida, M.; Sakagami, T.; Takeda, M.; Fukuchi, K.; Gomi, K.; Ohata, H.; Momose, K.; Kawazoe, Y.; Hatano, Tsutomu; Yoshida, T.; Okuda, T.

In: Anticancer Research, Vol. 15, No. 5 B, 1995, p. 2121-2128.

Research output: Contribution to journalArticle

Sakagami, H, Kuribayashi, N, Iida, M, Sakagami, T, Takeda, M, Fukuchi, K, Gomi, K, Ohata, H, Momose, K, Kawazoe, Y, Hatano, T, Yoshida, T & Okuda, T 1995, 'Induction of DNA fragmentation by tannin- and lignin-related substances', Anticancer Research, vol. 15, no. 5 B, pp. 2121-2128.
Sakagami H, Kuribayashi N, Iida M, Sakagami T, Takeda M, Fukuchi K et al. Induction of DNA fragmentation by tannin- and lignin-related substances. Anticancer Research. 1995;15(5 B):2121-2128.
Sakagami, H. ; Kuribayashi, N. ; Iida, M. ; Sakagami, T. ; Takeda, M. ; Fukuchi, K. ; Gomi, K. ; Ohata, H. ; Momose, K. ; Kawazoe, Y. ; Hatano, Tsutomu ; Yoshida, T. ; Okuda, T. / Induction of DNA fragmentation by tannin- and lignin-related substances. In: Anticancer Research. 1995 ; Vol. 15, No. 5 B. pp. 2121-2128.
@article{9e1e71d49d5941a7b16792ef71c297eb,
title = "Induction of DNA fragmentation by tannin- and lignin-related substances",
abstract = "A variety of tannin and lignin-related compounds were compared for their ability to induce nucleosome-sized DNA fragmentation (a biochemical hallmark of apoptosis), using agarose gel electrophoresis and a fluorescence activated cell sorter. Monomeric, dimeric, trimeric and tetrameric hydrolysable tannins induced nucleosome-sized DNA fragmentation in HL-60 cells, more potently than condensed tannins. The highest activity was detected in gallic acid, a component unit of tannins. Natural lignified materials, except for caffeic acid and its dehydrogenation polymer, showed much weaker activity. Protein-bound polysaccharide (PSK) was inactive. Gallic acid induced DNA fragmentation in four human myelogenous leukaemic cell lines, but not in human T-cell leukaemia and erythroleukaemia cell lines. Ca2+ depletion from the culture medium slightly, but significantly, reduced the apoptosis-inducing activity of gallic acid, but did not significantly affect that of tannic acid or caffeic acid. After treatment with gallic acid, intracellular Ca2+ concentration was significantly elevated. The apoptosis-inducing activity of polyphenols may further emphasize their medicinal efficacy.",
keywords = "Apoptosis, Calcium, DNA fragmentation, Lignin, Tannin",
author = "H. Sakagami and N. Kuribayashi and M. Iida and T. Sakagami and M. Takeda and K. Fukuchi and K. Gomi and H. Ohata and K. Momose and Y. Kawazoe and Tsutomu Hatano and T. Yoshida and T. Okuda",
year = "1995",
language = "English",
volume = "15",
pages = "2121--2128",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "5 B",

}

TY - JOUR

T1 - Induction of DNA fragmentation by tannin- and lignin-related substances

AU - Sakagami, H.

AU - Kuribayashi, N.

AU - Iida, M.

AU - Sakagami, T.

AU - Takeda, M.

AU - Fukuchi, K.

AU - Gomi, K.

AU - Ohata, H.

AU - Momose, K.

AU - Kawazoe, Y.

AU - Hatano, Tsutomu

AU - Yoshida, T.

AU - Okuda, T.

PY - 1995

Y1 - 1995

N2 - A variety of tannin and lignin-related compounds were compared for their ability to induce nucleosome-sized DNA fragmentation (a biochemical hallmark of apoptosis), using agarose gel electrophoresis and a fluorescence activated cell sorter. Monomeric, dimeric, trimeric and tetrameric hydrolysable tannins induced nucleosome-sized DNA fragmentation in HL-60 cells, more potently than condensed tannins. The highest activity was detected in gallic acid, a component unit of tannins. Natural lignified materials, except for caffeic acid and its dehydrogenation polymer, showed much weaker activity. Protein-bound polysaccharide (PSK) was inactive. Gallic acid induced DNA fragmentation in four human myelogenous leukaemic cell lines, but not in human T-cell leukaemia and erythroleukaemia cell lines. Ca2+ depletion from the culture medium slightly, but significantly, reduced the apoptosis-inducing activity of gallic acid, but did not significantly affect that of tannic acid or caffeic acid. After treatment with gallic acid, intracellular Ca2+ concentration was significantly elevated. The apoptosis-inducing activity of polyphenols may further emphasize their medicinal efficacy.

AB - A variety of tannin and lignin-related compounds were compared for their ability to induce nucleosome-sized DNA fragmentation (a biochemical hallmark of apoptosis), using agarose gel electrophoresis and a fluorescence activated cell sorter. Monomeric, dimeric, trimeric and tetrameric hydrolysable tannins induced nucleosome-sized DNA fragmentation in HL-60 cells, more potently than condensed tannins. The highest activity was detected in gallic acid, a component unit of tannins. Natural lignified materials, except for caffeic acid and its dehydrogenation polymer, showed much weaker activity. Protein-bound polysaccharide (PSK) was inactive. Gallic acid induced DNA fragmentation in four human myelogenous leukaemic cell lines, but not in human T-cell leukaemia and erythroleukaemia cell lines. Ca2+ depletion from the culture medium slightly, but significantly, reduced the apoptosis-inducing activity of gallic acid, but did not significantly affect that of tannic acid or caffeic acid. After treatment with gallic acid, intracellular Ca2+ concentration was significantly elevated. The apoptosis-inducing activity of polyphenols may further emphasize their medicinal efficacy.

KW - Apoptosis

KW - Calcium

KW - DNA fragmentation

KW - Lignin

KW - Tannin

UR - http://www.scopus.com/inward/record.url?scp=0029591280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029591280&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 2121

EP - 2128

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 5 B

ER -