Induction of defense responses in pea tissues by inorganic phosphate

Tomoharu Kawahara, Hiroko Namba, Kazuhiro Toyoda, Tomonari Kasai, Megumi Sugimoto, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

When inorganic phosphate, a common and essential element for organisms, was applied endogenously, a rejection reaction and superoxide generation were induced in pea tissues but phytoalexin production was not. Phosphate-induced superoxide generation was sensitive to cycloheximide (CHX) and salicylhydroxamic acid (SHAM), indicating that part of the generation was dependent upon the expression of peroxidase gene(s). Peroxidases (POXs) are well known not only to scavenge hydrogen peroxide with phenolics but also to generate superoxide via NADH oxidation in the presence of p-coumaric acid and manganese ion. We cloned five pea POX cDNAs that are predicted to be located outside of the cells. The accumulation of five POX mRNAs, NTPase mRNA, and phenylalanine ammonia-lyase mRNA was measured by semiquantitative reverse transcription-polymerase chain reaction. The expression of the five POX genes was induced by a fungal elicitor. On the other hand, inorganic phosphate induced the accumulation of POX11, POX14, and POX21 mRNAs but not of POX13, POX29, and PsPAL1 mRNAs within 1-3∈h after treatment of pea seedlings. In view of these findings, we discuss inorganic phosphate as a signal transmitter inducing part of the plant defense responses.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalJournal of General Plant Pathology
Volume72
Issue number3
DOIs
Publication statusPublished - Jun 2006

Fingerprint

peroxidases
peas
superoxide anion
phosphates
phytoalexins
p-coumaric acid
phenylalanine ammonia-lyase
cycloheximide
plant anatomy
manganese
hydrogen peroxide
peroxidase
genes
reverse transcriptase polymerase chain reaction
oxidation
ions
seedlings
tissues
acids
organisms

Keywords

  • Defense
  • Extracellular peroxidase
  • Inorganic phosphate signaling pathway
  • Mycosphaerella pinodes
  • Pisum sativum L.

ASJC Scopus subject areas

  • Plant Science

Cite this

Kawahara, T., Namba, H., Toyoda, K., Kasai, T., Sugimoto, M., Inagaki, Y., ... Shiraishi, T. (2006). Induction of defense responses in pea tissues by inorganic phosphate. Journal of General Plant Pathology, 72(3), 129-136. https://doi.org/10.1007/s10327-005-0261-9

Induction of defense responses in pea tissues by inorganic phosphate. / Kawahara, Tomoharu; Namba, Hiroko; Toyoda, Kazuhiro; Kasai, Tomonari; Sugimoto, Megumi; Inagaki, Yoshishige; Ichinose, Yuki; Shiraishi, Tomonori.

In: Journal of General Plant Pathology, Vol. 72, No. 3, 06.2006, p. 129-136.

Research output: Contribution to journalArticle

Kawahara, T, Namba, H, Toyoda, K, Kasai, T, Sugimoto, M, Inagaki, Y, Ichinose, Y & Shiraishi, T 2006, 'Induction of defense responses in pea tissues by inorganic phosphate', Journal of General Plant Pathology, vol. 72, no. 3, pp. 129-136. https://doi.org/10.1007/s10327-005-0261-9
Kawahara, Tomoharu ; Namba, Hiroko ; Toyoda, Kazuhiro ; Kasai, Tomonari ; Sugimoto, Megumi ; Inagaki, Yoshishige ; Ichinose, Yuki ; Shiraishi, Tomonori. / Induction of defense responses in pea tissues by inorganic phosphate. In: Journal of General Plant Pathology. 2006 ; Vol. 72, No. 3. pp. 129-136.
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