Abstract
When inorganic phosphate, a common and essential element for organisms, was applied endogenously, a rejection reaction and superoxide generation were induced in pea tissues but phytoalexin production was not. Phosphate-induced superoxide generation was sensitive to cycloheximide (CHX) and salicylhydroxamic acid (SHAM), indicating that part of the generation was dependent upon the expression of peroxidase gene(s). Peroxidases (POXs) are well known not only to scavenge hydrogen peroxide with phenolics but also to generate superoxide via NADH oxidation in the presence of p-coumaric acid and manganese ion. We cloned five pea POX cDNAs that are predicted to be located outside of the cells. The accumulation of five POX mRNAs, NTPase mRNA, and phenylalanine ammonia-lyase mRNA was measured by semiquantitative reverse transcription-polymerase chain reaction. The expression of the five POX genes was induced by a fungal elicitor. On the other hand, inorganic phosphate induced the accumulation of POX11, POX14, and POX21 mRNAs but not of POX13, POX29, and PsPAL1 mRNAs within 1-3∈h after treatment of pea seedlings. In view of these findings, we discuss inorganic phosphate as a signal transmitter inducing part of the plant defense responses.
| Original language | English |
|---|---|
| Pages (from-to) | 129-136 |
| Number of pages | 8 |
| Journal | Journal of General Plant Pathology |
| Volume | 72 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 2006 |
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Keywords
- Defense
- Extracellular peroxidase
- Inorganic phosphate signaling pathway
- Mycosphaerella pinodes
- Pisum sativum L.
ASJC Scopus subject areas
- Plant Science
Cite this
Induction of defense responses in pea tissues by inorganic phosphate. / Kawahara, Tomoharu; Namba, Hiroko; Toyoda, Kazuhiro; Kasai, Tomonari; Sugimoto, Megumi; Inagaki, Yoshishige; Ichinose, Yuki; Shiraishi, Tomonori.
In: Journal of General Plant Pathology, Vol. 72, No. 3, 06.2006, p. 129-136.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of defense responses in pea tissues by inorganic phosphate
AU - Kawahara, Tomoharu
AU - Namba, Hiroko
AU - Toyoda, Kazuhiro
AU - Kasai, Tomonari
AU - Sugimoto, Megumi
AU - Inagaki, Yoshishige
AU - Ichinose, Yuki
AU - Shiraishi, Tomonori
PY - 2006/6
Y1 - 2006/6
N2 - When inorganic phosphate, a common and essential element for organisms, was applied endogenously, a rejection reaction and superoxide generation were induced in pea tissues but phytoalexin production was not. Phosphate-induced superoxide generation was sensitive to cycloheximide (CHX) and salicylhydroxamic acid (SHAM), indicating that part of the generation was dependent upon the expression of peroxidase gene(s). Peroxidases (POXs) are well known not only to scavenge hydrogen peroxide with phenolics but also to generate superoxide via NADH oxidation in the presence of p-coumaric acid and manganese ion. We cloned five pea POX cDNAs that are predicted to be located outside of the cells. The accumulation of five POX mRNAs, NTPase mRNA, and phenylalanine ammonia-lyase mRNA was measured by semiquantitative reverse transcription-polymerase chain reaction. The expression of the five POX genes was induced by a fungal elicitor. On the other hand, inorganic phosphate induced the accumulation of POX11, POX14, and POX21 mRNAs but not of POX13, POX29, and PsPAL1 mRNAs within 1-3∈h after treatment of pea seedlings. In view of these findings, we discuss inorganic phosphate as a signal transmitter inducing part of the plant defense responses.
AB - When inorganic phosphate, a common and essential element for organisms, was applied endogenously, a rejection reaction and superoxide generation were induced in pea tissues but phytoalexin production was not. Phosphate-induced superoxide generation was sensitive to cycloheximide (CHX) and salicylhydroxamic acid (SHAM), indicating that part of the generation was dependent upon the expression of peroxidase gene(s). Peroxidases (POXs) are well known not only to scavenge hydrogen peroxide with phenolics but also to generate superoxide via NADH oxidation in the presence of p-coumaric acid and manganese ion. We cloned five pea POX cDNAs that are predicted to be located outside of the cells. The accumulation of five POX mRNAs, NTPase mRNA, and phenylalanine ammonia-lyase mRNA was measured by semiquantitative reverse transcription-polymerase chain reaction. The expression of the five POX genes was induced by a fungal elicitor. On the other hand, inorganic phosphate induced the accumulation of POX11, POX14, and POX21 mRNAs but not of POX13, POX29, and PsPAL1 mRNAs within 1-3∈h after treatment of pea seedlings. In view of these findings, we discuss inorganic phosphate as a signal transmitter inducing part of the plant defense responses.
KW - Defense
KW - Extracellular peroxidase
KW - Inorganic phosphate signaling pathway
KW - Mycosphaerella pinodes
KW - Pisum sativum L.
UR - http://www.scopus.com/inward/record.url?scp=33744490623&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33744490623&partnerID=8YFLogxK
U2 - 10.1007/s10327-005-0261-9
DO - 10.1007/s10327-005-0261-9
M3 - Article
VL - 72
SP - 129
EP - 136
JO - Journal of General Plant Pathology
JF - Journal of General Plant Pathology
SN - 1345-2630
IS - 3
ER -