Induction of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes

Akinori Kiba, Tadahiro Takeda, Takuya Kanemitsu, Kazuhiro Toyoda, Yuki Ichinose, Tetsuji Yamada, Tomonori Shiraishi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A high molecular weight elicitor (>70 kDa) from spore germination fluid of a pea pathogen, Mycosphaerella pinodes, has a partial structure of β-D-Glc-(1 → 6)-a-D-Man-(1 → 6)-D-Man, which is O-glycosidically attached to serine in the protein moiety. To elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including β-D-Glc-(1 → 6)-a-D-Man(1 → 6)-D-Man-O-Ser (No. 1) to [β-D-Glc-(1 → 6)-a-D-Man(1 → 6)-D-Man]3-O-Ser3-Pro3 (No. 9) on the infection by M. pinodes, superoxide generation and ATPase activity were measured. The glycoPePtides [β-D-Glc-(1 → 6)-a-D-Man-(1 → 6)-D-Man]-O-Ser2-Pro2 (No. 3) to No. 9 induced rejection reaction of pea tissue against M. pinodes. The glycopeptides No. 3 to No. 9 also induced superoxide generation on uninjured pea leaves. Moreover, the glycopeptides No. 3 to No. 9 induced in vitro the activation of cell wall-bound ATPase and superoxide generation system in the protein fraction solubilized from pea cell wall. The results indicate that the synthetic glycopeptides, No. 3 to No. 9, are available to analyze the signal transduction cascade leading to defense responses and the receptor for the elicitor.

Original languageEnglish
Pages (from-to)978-985
Number of pages8
JournalPlant and Cell Physiology
Volume40
Issue number9
Publication statusPublished - Sep 1999

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Keywords

  • Cell wall-bound ATPase
  • Mycosphaerella pinodes
  • Pisum sativum L.
  • Rejection reaction
  • Superoxide generation
  • Synthetic glycopeptide elicitor

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

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