Induction of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes

Akinori Kiba, Tadahiro Takeda, Takuya Kanemitsu, Kazuhiro Toyoda, Yuki Ichinose, Tetsuji Yamada, Tomonori Shiraishi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A high molecular weight elicitor (>70 kDa) from spore germination fluid of a pea pathogen, Mycosphaerella pinodes, has a partial structure of β-D-Glc-(1 → 6)-a-D-Man-(1 → 6)-D-Man, which is O-glycosidically attached to serine in the protein moiety. To elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including β-D-Glc-(1 → 6)-a-D-Man(1 → 6)-D-Man-O-Ser (No. 1) to [β-D-Glc-(1 → 6)-a-D-Man(1 → 6)-D-Man]3-O-Ser3-Pro3 (No. 9) on the infection by M. pinodes, superoxide generation and ATPase activity were measured. The glycoPePtides [β-D-Glc-(1 → 6)-a-D-Man-(1 → 6)-D-Man]-O-Ser2-Pro2 (No. 3) to No. 9 induced rejection reaction of pea tissue against M. pinodes. The glycopeptides No. 3 to No. 9 also induced superoxide generation on uninjured pea leaves. Moreover, the glycopeptides No. 3 to No. 9 induced in vitro the activation of cell wall-bound ATPase and superoxide generation system in the protein fraction solubilized from pea cell wall. The results indicate that the synthetic glycopeptides, No. 3 to No. 9, are available to analyze the signal transduction cascade leading to defense responses and the receptor for the elicitor.

Original languageEnglish
Pages (from-to)978-985
Number of pages8
JournalPlant and Cell Physiology
Volume40
Issue number9
Publication statusPublished - Sep 1999

Fingerprint

Mycosphaerella pinodes
glycopeptides
Glycopeptides
spore germination
Germination
Spores
Peas
peas
Superoxides
superoxide anion
Cell Wall
Adenosine Triphosphatases
adenosinetriphosphatase
cell walls
elicitors
fluids
Serine
Signal Transduction
serine
Proteins

Keywords

  • Cell wall-bound ATPase
  • Mycosphaerella pinodes
  • Pisum sativum L.
  • Rejection reaction
  • Superoxide generation
  • Synthetic glycopeptide elicitor

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Induction of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes. / Kiba, Akinori; Takeda, Tadahiro; Kanemitsu, Takuya; Toyoda, Kazuhiro; Ichinose, Yuki; Yamada, Tetsuji; Shiraishi, Tomonori.

In: Plant and Cell Physiology, Vol. 40, No. 9, 09.1999, p. 978-985.

Research output: Contribution to journalArticle

@article{33e9d2ff6602460abe1ca40fc993bfae,
title = "Induction of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes",
abstract = "A high molecular weight elicitor (>70 kDa) from spore germination fluid of a pea pathogen, Mycosphaerella pinodes, has a partial structure of β-D-Glc-(1 → 6)-a-D-Man-(1 → 6)-D-Man, which is O-glycosidically attached to serine in the protein moiety. To elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including β-D-Glc-(1 → 6)-a-D-Man(1 → 6)-D-Man-O-Ser (No. 1) to [β-D-Glc-(1 → 6)-a-D-Man(1 → 6)-D-Man]3-O-Ser3-Pro3 (No. 9) on the infection by M. pinodes, superoxide generation and ATPase activity were measured. The glycoPePtides [β-D-Glc-(1 → 6)-a-D-Man-(1 → 6)-D-Man]-O-Ser2-Pro2 (No. 3) to No. 9 induced rejection reaction of pea tissue against M. pinodes. The glycopeptides No. 3 to No. 9 also induced superoxide generation on uninjured pea leaves. Moreover, the glycopeptides No. 3 to No. 9 induced in vitro the activation of cell wall-bound ATPase and superoxide generation system in the protein fraction solubilized from pea cell wall. The results indicate that the synthetic glycopeptides, No. 3 to No. 9, are available to analyze the signal transduction cascade leading to defense responses and the receptor for the elicitor.",
keywords = "Cell wall-bound ATPase, Mycosphaerella pinodes, Pisum sativum L., Rejection reaction, Superoxide generation, Synthetic glycopeptide elicitor",
author = "Akinori Kiba and Tadahiro Takeda and Takuya Kanemitsu and Kazuhiro Toyoda and Yuki Ichinose and Tetsuji Yamada and Tomonori Shiraishi",
year = "1999",
month = "9",
language = "English",
volume = "40",
pages = "978--985",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "9",

}

TY - JOUR

T1 - Induction of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes

AU - Kiba, Akinori

AU - Takeda, Tadahiro

AU - Kanemitsu, Takuya

AU - Toyoda, Kazuhiro

AU - Ichinose, Yuki

AU - Yamada, Tetsuji

AU - Shiraishi, Tomonori

PY - 1999/9

Y1 - 1999/9

N2 - A high molecular weight elicitor (>70 kDa) from spore germination fluid of a pea pathogen, Mycosphaerella pinodes, has a partial structure of β-D-Glc-(1 → 6)-a-D-Man-(1 → 6)-D-Man, which is O-glycosidically attached to serine in the protein moiety. To elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including β-D-Glc-(1 → 6)-a-D-Man(1 → 6)-D-Man-O-Ser (No. 1) to [β-D-Glc-(1 → 6)-a-D-Man(1 → 6)-D-Man]3-O-Ser3-Pro3 (No. 9) on the infection by M. pinodes, superoxide generation and ATPase activity were measured. The glycoPePtides [β-D-Glc-(1 → 6)-a-D-Man-(1 → 6)-D-Man]-O-Ser2-Pro2 (No. 3) to No. 9 induced rejection reaction of pea tissue against M. pinodes. The glycopeptides No. 3 to No. 9 also induced superoxide generation on uninjured pea leaves. Moreover, the glycopeptides No. 3 to No. 9 induced in vitro the activation of cell wall-bound ATPase and superoxide generation system in the protein fraction solubilized from pea cell wall. The results indicate that the synthetic glycopeptides, No. 3 to No. 9, are available to analyze the signal transduction cascade leading to defense responses and the receptor for the elicitor.

AB - A high molecular weight elicitor (>70 kDa) from spore germination fluid of a pea pathogen, Mycosphaerella pinodes, has a partial structure of β-D-Glc-(1 → 6)-a-D-Man-(1 → 6)-D-Man, which is O-glycosidically attached to serine in the protein moiety. To elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including β-D-Glc-(1 → 6)-a-D-Man(1 → 6)-D-Man-O-Ser (No. 1) to [β-D-Glc-(1 → 6)-a-D-Man(1 → 6)-D-Man]3-O-Ser3-Pro3 (No. 9) on the infection by M. pinodes, superoxide generation and ATPase activity were measured. The glycoPePtides [β-D-Glc-(1 → 6)-a-D-Man-(1 → 6)-D-Man]-O-Ser2-Pro2 (No. 3) to No. 9 induced rejection reaction of pea tissue against M. pinodes. The glycopeptides No. 3 to No. 9 also induced superoxide generation on uninjured pea leaves. Moreover, the glycopeptides No. 3 to No. 9 induced in vitro the activation of cell wall-bound ATPase and superoxide generation system in the protein fraction solubilized from pea cell wall. The results indicate that the synthetic glycopeptides, No. 3 to No. 9, are available to analyze the signal transduction cascade leading to defense responses and the receptor for the elicitor.

KW - Cell wall-bound ATPase

KW - Mycosphaerella pinodes

KW - Pisum sativum L.

KW - Rejection reaction

KW - Superoxide generation

KW - Synthetic glycopeptide elicitor

UR - http://www.scopus.com/inward/record.url?scp=0033198624&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033198624&partnerID=8YFLogxK

M3 - Article

VL - 40

SP - 978

EP - 985

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 9

ER -