Induction of cytochrome P-450, cytochrome b-5, nadph-cytochrome c reductase and change of cytochrome P-450 isozymes with long-term trichloroethylene treatment

Kawamoto Toshihiro Kawamoto, Hobara Tatsya Hobara, Nakamura Kazuyuki Nakamura, Imamura Akihisa Imamura, Keiki Ogino, Kobayashi Haruo Kobayashi, Iwamoto Susumu Iwamoto, Sakai Tsunemi Sakai

Research output: Contribution to journalArticle

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Abstract

Several reports have described the effects of trichloroethylene (TCE) on the microsomal mixed function oxidase system (MFOS). These studies suggest that repeated TCE administration induces MFOS, especially cytochrome P-450 and NADPH-cytochrome c reductase. However, it is uncertain what isozymes are induced by TCE treatment, and it is not clear how microsomal enzymes or cytochrome P-450 isozymes are altered when TCE is administered for a duration longer than 28 days. We investigated the changes of MFOS by long-term TCE treatment. Male Wistar rats were injected TCE, 1.0 k/kg body weight once a day for 5 continuous days or 2.0 g/kg body weight twice a week for 15 days. The mean body weight of the rats treated with TCE for 15 weeks was slightly, but not significantly, less than that of the control rats. Relative liver weights (liver wt/body wt) of the TCE-treated group were however significantly larger (21 %) than those of the control group. The weights of the other organs were not changed by long-term TCE treatment. Trichloroethylene treatments for 5 days and 15 weeks caused significant increases in microsomal protein, cytochrome P-450, cytochrome b-5 and NADPH-cytochrome c reductase. TCE treatments produced an increase in a polypeptide band at 52 000 molecular weight range observed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This increase is similar to, but less pronounced than that induced by phenobarbital (PB) treatment. There were no remarkable changes at 56 000 molecular weight range where a band appeared after the treatment with 3-methylcholanthrene (MC). It is likely that the induction of cytochrome P-450 by TCE is relatively similar to that by PB.

Original languageEnglish
Pages (from-to)239-249
Number of pages11
JournalToxicology
Volume53
Issue number2-3
DOIs
Publication statusPublished - Dec 30 1988
Externally publishedYes

Fingerprint

Cytochrome Reductases
Cytochromes b5
Trichloroethylene
NADPH-Ferrihemoprotein Reductase
Cytochromes c
Cytochrome P-450 Enzyme System
Isoenzymes
Mixed Function Oxygenases
Body Weight
Phenobarbital
Liver
Rats
Molecular Weight
Molecular weight
Rat control
Methylcholanthrene
Organ Size
Electrophoresis
Sodium Dodecyl Sulfate
Wistar Rats

Keywords

  • Cytochrome b-5
  • Cytochrome P-450
  • Induction
  • Isozymes
  • NADPH-cytochrome c reductase
  • Trichloroethylene

ASJC Scopus subject areas

  • Toxicology

Cite this

Toshihiro Kawamoto, K., Tatsya Hobara, H., Kazuyuki Nakamura, N., Akihisa Imamura, I., Ogino, K., Haruo Kobayashi, K., ... Tsunemi Sakai, S. (1988). Induction of cytochrome P-450, cytochrome b-5, nadph-cytochrome c reductase and change of cytochrome P-450 isozymes with long-term trichloroethylene treatment. Toxicology, 53(2-3), 239-249. https://doi.org/10.1016/0300-483X(88)90217-X

Induction of cytochrome P-450, cytochrome b-5, nadph-cytochrome c reductase and change of cytochrome P-450 isozymes with long-term trichloroethylene treatment. / Toshihiro Kawamoto, Kawamoto; Tatsya Hobara, Hobara; Kazuyuki Nakamura, Nakamura; Akihisa Imamura, Imamura; Ogino, Keiki; Haruo Kobayashi, Kobayashi; Susumu Iwamoto, Iwamoto; Tsunemi Sakai, Sakai.

In: Toxicology, Vol. 53, No. 2-3, 30.12.1988, p. 239-249.

Research output: Contribution to journalArticle

Toshihiro Kawamoto, K, Tatsya Hobara, H, Kazuyuki Nakamura, N, Akihisa Imamura, I, Ogino, K, Haruo Kobayashi, K, Susumu Iwamoto, I & Tsunemi Sakai, S 1988, 'Induction of cytochrome P-450, cytochrome b-5, nadph-cytochrome c reductase and change of cytochrome P-450 isozymes with long-term trichloroethylene treatment', Toxicology, vol. 53, no. 2-3, pp. 239-249. https://doi.org/10.1016/0300-483X(88)90217-X
Toshihiro Kawamoto K, Tatsya Hobara H, Kazuyuki Nakamura N, Akihisa Imamura I, Ogino K, Haruo Kobayashi K et al. Induction of cytochrome P-450, cytochrome b-5, nadph-cytochrome c reductase and change of cytochrome P-450 isozymes with long-term trichloroethylene treatment. Toxicology. 1988 Dec 30;53(2-3):239-249. https://doi.org/10.1016/0300-483X(88)90217-X
Toshihiro Kawamoto, Kawamoto ; Tatsya Hobara, Hobara ; Kazuyuki Nakamura, Nakamura ; Akihisa Imamura, Imamura ; Ogino, Keiki ; Haruo Kobayashi, Kobayashi ; Susumu Iwamoto, Iwamoto ; Tsunemi Sakai, Sakai. / Induction of cytochrome P-450, cytochrome b-5, nadph-cytochrome c reductase and change of cytochrome P-450 isozymes with long-term trichloroethylene treatment. In: Toxicology. 1988 ; Vol. 53, No. 2-3. pp. 239-249.
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abstract = "Several reports have described the effects of trichloroethylene (TCE) on the microsomal mixed function oxidase system (MFOS). These studies suggest that repeated TCE administration induces MFOS, especially cytochrome P-450 and NADPH-cytochrome c reductase. However, it is uncertain what isozymes are induced by TCE treatment, and it is not clear how microsomal enzymes or cytochrome P-450 isozymes are altered when TCE is administered for a duration longer than 28 days. We investigated the changes of MFOS by long-term TCE treatment. Male Wistar rats were injected TCE, 1.0 k/kg body weight once a day for 5 continuous days or 2.0 g/kg body weight twice a week for 15 days. The mean body weight of the rats treated with TCE for 15 weeks was slightly, but not significantly, less than that of the control rats. Relative liver weights (liver wt/body wt) of the TCE-treated group were however significantly larger (21 {\%}) than those of the control group. The weights of the other organs were not changed by long-term TCE treatment. Trichloroethylene treatments for 5 days and 15 weeks caused significant increases in microsomal protein, cytochrome P-450, cytochrome b-5 and NADPH-cytochrome c reductase. TCE treatments produced an increase in a polypeptide band at 52 000 molecular weight range observed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This increase is similar to, but less pronounced than that induced by phenobarbital (PB) treatment. There were no remarkable changes at 56 000 molecular weight range where a band appeared after the treatment with 3-methylcholanthrene (MC). It is likely that the induction of cytochrome P-450 by TCE is relatively similar to that by PB.",
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