Induction of chemosensitivity in human lung cancer cells in vivo by adenovirus-mediated transfer of the wild-type p53 gene

Toshiyoshi Fujiwara, Elizabeth A. Grimm, Tapas Mukhopadhyay, Wei Wei Zhang, Laurie B. Owen-Schaub, Jack A. Roth

Research output: Contribution to journalArticle

480 Citations (Scopus)

Abstract

Recombinant adenovirus-mediated transfer of the wild-type p53 gene into monolayer cultures or multicellular tumor spheroids of human non-small cell lung cancer cell line H358, which has a homozygous deletion of p53, markedly increased the cellular sensitivity of these cells to the chemotherapeutic drug cisplatin. Treated cells underwent apoptosis with specific DNA fragmentation. Direct injection of the p53-adenovirus construct into H358 tumors s.c. implanted into nu/nu mice, followed by i.p. administration of cisplatin, induced massive apoptotic destruction of the tumors. These results support the clinical application of a regimen combining gene replacement using replication-deficient wild-type p53 adenovirus and DNA-damaging drugs for treatment of human cancer.

Original languageEnglish
Pages (from-to)2287-2291
Number of pages5
JournalCancer Research
Volume54
Issue number9
Publication statusPublished - May 1 1994
Externally publishedYes

Fingerprint

p53 Genes
Adenoviridae
Lung Neoplasms
Cisplatin
Neoplasms
Cellular Spheroids
DNA Fragmentation
Non-Small Cell Lung Carcinoma
Pharmaceutical Preparations
Apoptosis
Cell Line
Injections
DNA
Genes
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Fujiwara, T., Grimm, E. A., Mukhopadhyay, T., Zhang, W. W., Owen-Schaub, L. B., & Roth, J. A. (1994). Induction of chemosensitivity in human lung cancer cells in vivo by adenovirus-mediated transfer of the wild-type p53 gene. Cancer Research, 54(9), 2287-2291.

Induction of chemosensitivity in human lung cancer cells in vivo by adenovirus-mediated transfer of the wild-type p53 gene. / Fujiwara, Toshiyoshi; Grimm, Elizabeth A.; Mukhopadhyay, Tapas; Zhang, Wei Wei; Owen-Schaub, Laurie B.; Roth, Jack A.

In: Cancer Research, Vol. 54, No. 9, 01.05.1994, p. 2287-2291.

Research output: Contribution to journalArticle

Fujiwara, T, Grimm, EA, Mukhopadhyay, T, Zhang, WW, Owen-Schaub, LB & Roth, JA 1994, 'Induction of chemosensitivity in human lung cancer cells in vivo by adenovirus-mediated transfer of the wild-type p53 gene', Cancer Research, vol. 54, no. 9, pp. 2287-2291.
Fujiwara, Toshiyoshi ; Grimm, Elizabeth A. ; Mukhopadhyay, Tapas ; Zhang, Wei Wei ; Owen-Schaub, Laurie B. ; Roth, Jack A. / Induction of chemosensitivity in human lung cancer cells in vivo by adenovirus-mediated transfer of the wild-type p53 gene. In: Cancer Research. 1994 ; Vol. 54, No. 9. pp. 2287-2291.
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