Induction of c-fos transcription in the medaka brain (Oryzias latipes) in response to mating stimuli

Teruhiro Okuyama, Yuji Suehiro, Haruka Imada, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Takeo Kubo, Hideaki Takeuchi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Immediate-early genes (IEGs) are useful for mapping active brain regions in various vertebrates. Here we identified a c- fos homologue gene in medaka and demonstrated that the amounts of c-fos transcripts and proteins in the medaka brain increased in relation to an artificially evoked seizure, suggesting that the homologue gene has the characteristics of IEGs, which are used as markers of neural activity. Next, quantitative reverse-transcription-polymerase chain reaction revealed that female mating behaviors upregulated c-fos transcription in some brain regions including the telencephalon, optic tectum, and cerebellum. In addition, we performed in situ hybridization with a c-fos intron probe to detect the de novo synthesis of c-fos transcripts and confirmed induction of c-fos transcription in these brain regions after mating. This is the first report of IEG induction in response to mating stimuli in teleost fish. Our results indicated that c-fos expression was induced in response to behavioral stimuli in the medaka brain and that medaka c-fos could be a useful marker of neural activity.

Original languageEnglish
Pages (from-to)453-457
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume404
Issue number1
DOIs
Publication statusPublished - Jan 7 2011
Externally publishedYes

Fingerprint

Oryzias
Transcription
Brain
Genes
Immediate-Early Genes
Proto-Oncogene Proteins c-fos
fos Genes
Telencephalon
Superior Colliculi
Chromosome Mapping
Polymerase chain reaction
Introns
Fish
Cerebellum
Reverse Transcription
In Situ Hybridization
Vertebrates
Optics
Fishes
Seizures

Keywords

  • C-fos
  • Mating behavior
  • Medaka
  • Telencephalon
  • Teleost

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Induction of c-fos transcription in the medaka brain (Oryzias latipes) in response to mating stimuli. / Okuyama, Teruhiro; Suehiro, Yuji; Imada, Haruka; Shimada, Atsuko; Naruse, Kiyoshi; Takeda, Hiroyuki; Kubo, Takeo; Takeuchi, Hideaki.

In: Biochemical and Biophysical Research Communications, Vol. 404, No. 1, 07.01.2011, p. 453-457.

Research output: Contribution to journalArticle

Okuyama, Teruhiro ; Suehiro, Yuji ; Imada, Haruka ; Shimada, Atsuko ; Naruse, Kiyoshi ; Takeda, Hiroyuki ; Kubo, Takeo ; Takeuchi, Hideaki. / Induction of c-fos transcription in the medaka brain (Oryzias latipes) in response to mating stimuli. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 404, No. 1. pp. 453-457.
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