Induction of apoptosis by β-carotene and dimethyl tetrasulfide assisted by UVA irradiation in HL-60 Cells

Gongliang Zhang, Haitao Wu, Beiwei Zhu, Yasuaki Shimoishi, Yoshimasa Nakamura, Yoshiyuki Murata

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Cellular phototoxicity induced by UVA irradiation and its potential application to therapy has been reported. In the present study, the induction of apoptosis induced by β-carotene and dimethyl tetrasulfide (Me2S4) assisted by UVA irradiation in HL-60 cells was assessed. β-carotene assisted by UVA significantly decreased the cell viability and induced DNA fragmentation in HL-60 cells. Me2S4 combined with β-carotene and assisted by UVA significantly inhibited the cell viability, and enhanced the caspase-3 activity which was completely inhibited by N-acety-L-cysteine. β-carotene was significantly degraded by UVA, but this was not accelerated by Me2S4 in a cell culture system. The photodegradation products of β-carotene prepared by UVA irradiation regardless of the addition of Me2S4 showed lower cytotoxicity than β-carotene itself in HL-60 cells. These results suggest that the ROS-and caspase-3-dependent apoptosis induced by β-carotene and Me2S4 assisted by UVA was due to a synergistic action rather than to the sole effect of the photodegradation products of β-carotene in HL-60 cells.

Original languageEnglish
Pages (from-to)1014-1020
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Issue number5
Publication statusPublished - 2009



  • β-carotene
  • Apoptosis
  • Dimethyl tetrasulfide
  • HL-60 cells
  • UVA

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

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