Induction of an outer membrane protein of 78 kDa in Vibrio vulnificus cultured in the presence of desferrioxamine B under iron-limiting conditions

Hiroaki Aso, Shin Ichi Miyoshi, Hiroshi Nakao, Keinosuke Okamoto, Shigeo Yamamoto

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Although Vibrio vulnificus is known to be able to utilize ferrioxamine B as an iron source, its outer membrane receptor remains to be determined. In this study, we found that V. vulnificus expressed a new outer membrane protein of 78 kDa when grown in the presence of desferrioxamine B under iron-limiting conditions. The desferrioxamine B-dependent iron uptake was only observed in bacterial cells expressing this protein. Furthermore, non-denaturing polyacrylamide gel electrophoresis followed by autoradiography of the outer membrane preparation containing the 78-kDa protein preincubated with [55Fe]ferrioxamine B provided a single radioactive band in which the 78-kDa outer membrane protein was present as the major component. These lines of evidence suggest that the inducible 78-kDa protein may serve as the cell-surface receptor for ferrioxamine B.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalFEMS Microbiology Letters
Volume212
Issue number1
DOIs
Publication statusPublished - Jun 18 2002

Keywords

  • Desferal
  • Ferrioxamine B
  • Siderophore receptor induction
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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