Induction of active invagination of plasma membranes by Helicobacter pylori cytotoxin, VacA, in cultured gastric cells: An immunoelectron microscopic study

Shin Ichi Izumi, Miyuki Kimura, Takahiro Kimura, Akihiro Wada, Toshiya Hirayama, Akitoyo Ichinose, Takehiko Koji

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

VacA cytotoxin secreted by Helicobacter pylori causes cytoplasmic vacuolation in gastric epithelial cells. To elucidate whether VacA directly affects the cell surface, changes in the membrane structure after VacA treatment were examined in a human gastric epithelial cell culture (AZ-521) at an ultrastructural level. When the time-course changes in the localization of VacA were analyzed by immunoelectron microscopy in AZ-521 cells that had been exposed to VacA, VacA was detected predominantly on the plasma membrane at an early stage of the exposure, and later in endocytotic vesicles and/or tubulo-vesicles. Five to 24 hr after exposure, the VacA was distributed in a limestone cavern like-structure, which was formed by elongated surfaces of plasma membranes, as shown by scanning electron microscopy. Therefore, the binding of VacA to the plasma membrane and the subsequent internalization into the enlarged cavern like-plasma membranes may be involved in the induction of vacuolation of gastric epithelial cells by VacA.

Original languageEnglish
Pages (from-to)89-94
Number of pages6
JournalActa Histochemica et Cytochemica
Volume33
Issue number2
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Cytotoxins
Cell membranes
Helicobacter pylori
Cultured Cells
Stomach
Cell Membrane
Epithelial Cells
Membrane structures
Immunoelectron Microscopy
Calcium Carbonate
Cell culture
Electron Scanning Microscopy
Microscopic examination
Cell Culture Techniques
Scanning electron microscopy
Membranes

Keywords

  • Electron microscopy
  • Helicobacter pylori
  • Immunohistochemistry
  • Plasma membrane
  • VacA

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology
  • Anatomy
  • Neuroscience(all)

Cite this

Induction of active invagination of plasma membranes by Helicobacter pylori cytotoxin, VacA, in cultured gastric cells : An immunoelectron microscopic study. / Izumi, Shin Ichi; Kimura, Miyuki; Kimura, Takahiro; Wada, Akihiro; Hirayama, Toshiya; Ichinose, Akitoyo; Koji, Takehiko.

In: Acta Histochemica et Cytochemica, Vol. 33, No. 2, 2000, p. 89-94.

Research output: Contribution to journalArticle

Izumi, Shin Ichi ; Kimura, Miyuki ; Kimura, Takahiro ; Wada, Akihiro ; Hirayama, Toshiya ; Ichinose, Akitoyo ; Koji, Takehiko. / Induction of active invagination of plasma membranes by Helicobacter pylori cytotoxin, VacA, in cultured gastric cells : An immunoelectron microscopic study. In: Acta Histochemica et Cytochemica. 2000 ; Vol. 33, No. 2. pp. 89-94.
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