Induction and prevention of virus-associated malignant lymphoma by serial transmission of EBV-related virus from cynomolgus by blood transfusion in rabbits

Tirtha Raj Koirala, Kazuhiko Hayashi, Zaishun Jin, Sachiyo Onoda, Takehiro Tanaka, Wakako Oda, Koichi Ichimura, Nobuya Ohara, Takashi Oka, Masao Yamada, Tadashi Yoshino

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV)-related herpesvirus (Si-IIA-EBV) was serially transmitted for 3 passages from rabbit to rabbit of the opposite sex by blood transfusion, which subsequently induced virus-associated rabbit lymphomas. The virus could be transmitted by transfusion with 15-20 ml of whole blood (7/7) or irradiated blood (1/6) from the EBV-related virus-infected rabbits, but there was no transmission with transfusion of cell-free plasma (0/6) from the infected rabbits. Passive anti-EBV-VCA IgG (x 20-x 10) titers decreased during the first 1-2 weeks in the transfused rabbits. The virus-transmitted rabbits showed a gradual increase in antibody titers ranging from peak titers of x 640 to x 2560 after 3 weeks of transfusion. The recipient origin of malignant lymphoma that developed in the first rabbit transfused by infected blood was confirmed by chromosomal analysis. This rabbit model thus shows that EBV-related herpesvirus is serially transmissible by blood transfusion and that transmission can not be completely prevented by irradiation of blood, but removal of blood cells is the best way to prevent transmission of EBV-related virus. Therefore, this animal model provides a convenient in vivo system for studies of the prevention and therapy of transfusion-related transmission of EBV and EBV-associated lymphoproliferative diseases in immunocompromised human beings.

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalActa Medica Okayama
Volume58
Issue number2
Publication statusPublished - Apr 2004

Fingerprint

Virus Activation
Human Herpesvirus 4
Viruses
Blood Transfusion
Lymphoma
Blood
Rabbits
Herpesviridae
Plasma Cells
Blood Cells
Animal Models
Immunoglobulin G

Keywords

  • Blood transfusion
  • Epstein-Barr virus (EBV)
  • Lymphoproliferative diseases
  • Rabbit

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Induction and prevention of virus-associated malignant lymphoma by serial transmission of EBV-related virus from cynomolgus by blood transfusion in rabbits. / Koirala, Tirtha Raj; Hayashi, Kazuhiko; Jin, Zaishun; Onoda, Sachiyo; Tanaka, Takehiro; Oda, Wakako; Ichimura, Koichi; Ohara, Nobuya; Oka, Takashi; Yamada, Masao; Yoshino, Tadashi.

In: Acta Medica Okayama, Vol. 58, No. 2, 04.2004, p. 67-74.

Research output: Contribution to journalArticle

Koirala, Tirtha Raj ; Hayashi, Kazuhiko ; Jin, Zaishun ; Onoda, Sachiyo ; Tanaka, Takehiro ; Oda, Wakako ; Ichimura, Koichi ; Ohara, Nobuya ; Oka, Takashi ; Yamada, Masao ; Yoshino, Tadashi. / Induction and prevention of virus-associated malignant lymphoma by serial transmission of EBV-related virus from cynomolgus by blood transfusion in rabbits. In: Acta Medica Okayama. 2004 ; Vol. 58, No. 2. pp. 67-74.
@article{e8d6dd583d3343549400b96773e240cb,
title = "Induction and prevention of virus-associated malignant lymphoma by serial transmission of EBV-related virus from cynomolgus by blood transfusion in rabbits",
abstract = "Epstein-Barr virus (EBV)-related herpesvirus (Si-IIA-EBV) was serially transmitted for 3 passages from rabbit to rabbit of the opposite sex by blood transfusion, which subsequently induced virus-associated rabbit lymphomas. The virus could be transmitted by transfusion with 15-20 ml of whole blood (7/7) or irradiated blood (1/6) from the EBV-related virus-infected rabbits, but there was no transmission with transfusion of cell-free plasma (0/6) from the infected rabbits. Passive anti-EBV-VCA IgG (x 20-x 10) titers decreased during the first 1-2 weeks in the transfused rabbits. The virus-transmitted rabbits showed a gradual increase in antibody titers ranging from peak titers of x 640 to x 2560 after 3 weeks of transfusion. The recipient origin of malignant lymphoma that developed in the first rabbit transfused by infected blood was confirmed by chromosomal analysis. This rabbit model thus shows that EBV-related herpesvirus is serially transmissible by blood transfusion and that transmission can not be completely prevented by irradiation of blood, but removal of blood cells is the best way to prevent transmission of EBV-related virus. Therefore, this animal model provides a convenient in vivo system for studies of the prevention and therapy of transfusion-related transmission of EBV and EBV-associated lymphoproliferative diseases in immunocompromised human beings.",
keywords = "Blood transfusion, Epstein-Barr virus (EBV), Lymphoproliferative diseases, Rabbit",
author = "Koirala, {Tirtha Raj} and Kazuhiko Hayashi and Zaishun Jin and Sachiyo Onoda and Takehiro Tanaka and Wakako Oda and Koichi Ichimura and Nobuya Ohara and Takashi Oka and Masao Yamada and Tadashi Yoshino",
year = "2004",
month = "4",
language = "English",
volume = "58",
pages = "67--74",
journal = "Acta Medica Okayama",
issn = "0386-300X",
publisher = "Okayama University",
number = "2",

}

TY - JOUR

T1 - Induction and prevention of virus-associated malignant lymphoma by serial transmission of EBV-related virus from cynomolgus by blood transfusion in rabbits

AU - Koirala, Tirtha Raj

AU - Hayashi, Kazuhiko

AU - Jin, Zaishun

AU - Onoda, Sachiyo

AU - Tanaka, Takehiro

AU - Oda, Wakako

AU - Ichimura, Koichi

AU - Ohara, Nobuya

AU - Oka, Takashi

AU - Yamada, Masao

AU - Yoshino, Tadashi

PY - 2004/4

Y1 - 2004/4

N2 - Epstein-Barr virus (EBV)-related herpesvirus (Si-IIA-EBV) was serially transmitted for 3 passages from rabbit to rabbit of the opposite sex by blood transfusion, which subsequently induced virus-associated rabbit lymphomas. The virus could be transmitted by transfusion with 15-20 ml of whole blood (7/7) or irradiated blood (1/6) from the EBV-related virus-infected rabbits, but there was no transmission with transfusion of cell-free plasma (0/6) from the infected rabbits. Passive anti-EBV-VCA IgG (x 20-x 10) titers decreased during the first 1-2 weeks in the transfused rabbits. The virus-transmitted rabbits showed a gradual increase in antibody titers ranging from peak titers of x 640 to x 2560 after 3 weeks of transfusion. The recipient origin of malignant lymphoma that developed in the first rabbit transfused by infected blood was confirmed by chromosomal analysis. This rabbit model thus shows that EBV-related herpesvirus is serially transmissible by blood transfusion and that transmission can not be completely prevented by irradiation of blood, but removal of blood cells is the best way to prevent transmission of EBV-related virus. Therefore, this animal model provides a convenient in vivo system for studies of the prevention and therapy of transfusion-related transmission of EBV and EBV-associated lymphoproliferative diseases in immunocompromised human beings.

AB - Epstein-Barr virus (EBV)-related herpesvirus (Si-IIA-EBV) was serially transmitted for 3 passages from rabbit to rabbit of the opposite sex by blood transfusion, which subsequently induced virus-associated rabbit lymphomas. The virus could be transmitted by transfusion with 15-20 ml of whole blood (7/7) or irradiated blood (1/6) from the EBV-related virus-infected rabbits, but there was no transmission with transfusion of cell-free plasma (0/6) from the infected rabbits. Passive anti-EBV-VCA IgG (x 20-x 10) titers decreased during the first 1-2 weeks in the transfused rabbits. The virus-transmitted rabbits showed a gradual increase in antibody titers ranging from peak titers of x 640 to x 2560 after 3 weeks of transfusion. The recipient origin of malignant lymphoma that developed in the first rabbit transfused by infected blood was confirmed by chromosomal analysis. This rabbit model thus shows that EBV-related herpesvirus is serially transmissible by blood transfusion and that transmission can not be completely prevented by irradiation of blood, but removal of blood cells is the best way to prevent transmission of EBV-related virus. Therefore, this animal model provides a convenient in vivo system for studies of the prevention and therapy of transfusion-related transmission of EBV and EBV-associated lymphoproliferative diseases in immunocompromised human beings.

KW - Blood transfusion

KW - Epstein-Barr virus (EBV)

KW - Lymphoproliferative diseases

KW - Rabbit

UR - http://www.scopus.com/inward/record.url?scp=3042681117&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042681117&partnerID=8YFLogxK

M3 - Article

C2 - 15255507

AN - SCOPUS:3042681117

VL - 58

SP - 67

EP - 74

JO - Acta Medica Okayama

JF - Acta Medica Okayama

SN - 0386-300X

IS - 2

ER -