Induced accessibility and enhanced inaccessibility at the cellular level in barley coleoptiles. V. Duration of stimulus by a non-pathogen in relation to enhanced inaccessibility

Hitoshi Kunoh, Kazuhiro Toyoda, Naoto Yamaoka, Issei Kobayashi

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Abstract

The influence of the duration of a direct stimulus by a non-pathogen to the enhancement of inaccessibility was examined using barley coleoptile cells and Erysiphe pisi. The degree of enhanced inaccessibility was evaluated with the rate of haustorium formation (penetration efficiency, PE) of E. graminis which attempted penetration of the same coleoptile cells as E. pisi. Partially dissected coleoptiles (coleoptiles A) were inoculated with E. pisi conidia. By observing the specimens every 15 min, the time of initiation of cytoplasmic aggregation in coleoptile cells below an individual E. pisi appressorium was recorded. At 0·5, 1·0 or 3·0 h after the initiation of cytoplasmic aggregation, germlings of E. pisi were removed by a micromanipulator. Germinating conidia of E. graminis which had been inoculated onto other coleoptiles were then transferred onto coleoptile A by micromanipulation so that they could attempt to penetrate the same cells in which E. pisi had induced a cytoplasmic aggregate. When E. pisi was removed 0·5 h after the initiation of cytoplasmic aggregation, the PE of E. graminis was reduced by 35·4%, suggesting that the inaccessibility was enhanced by the presence of E. pisi. When E. pisi was removed at 1·0 and 3·0 h, the subsequent PEs of E. graminis were 24·8% and 5·8%, respectively, indicating that the prolonged presence of E. pisi further enhanced the inaccessibility. The degree of enhanced inaccessibility was influenced by the size of coleoptile cells but not by the distances between penetration sites of the two fungi within the same cell and the time intervals of the initiation of cytoplasmic aggregation induced by both fungi. It is suggested that unknown mechanism(s) unrelated to papilla formation may be involved in the enhancement of inaccessibility.

Original languageEnglish
Pages (from-to)507-518
Number of pages12
JournalPhysiological and Molecular Plant Pathology
Volume35
Issue number6
DOIs
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Erysiphe pisi
Cotyledon
coleoptiles
Hordeum
barley
duration
Fungal Spores
cells
Fungi
conidia
Micromanipulation
Blumeria graminis
Cell Size
fungi
appressoria
cell aggregates

ASJC Scopus subject areas

  • Plant Science

Cite this

@article{f227a48e8e224ed6a377363ec8d38793,
title = "Induced accessibility and enhanced inaccessibility at the cellular level in barley coleoptiles. V. Duration of stimulus by a non-pathogen in relation to enhanced inaccessibility",
abstract = "The influence of the duration of a direct stimulus by a non-pathogen to the enhancement of inaccessibility was examined using barley coleoptile cells and Erysiphe pisi. The degree of enhanced inaccessibility was evaluated with the rate of haustorium formation (penetration efficiency, PE) of E. graminis which attempted penetration of the same coleoptile cells as E. pisi. Partially dissected coleoptiles (coleoptiles A) were inoculated with E. pisi conidia. By observing the specimens every 15 min, the time of initiation of cytoplasmic aggregation in coleoptile cells below an individual E. pisi appressorium was recorded. At 0·5, 1·0 or 3·0 h after the initiation of cytoplasmic aggregation, germlings of E. pisi were removed by a micromanipulator. Germinating conidia of E. graminis which had been inoculated onto other coleoptiles were then transferred onto coleoptile A by micromanipulation so that they could attempt to penetrate the same cells in which E. pisi had induced a cytoplasmic aggregate. When E. pisi was removed 0·5 h after the initiation of cytoplasmic aggregation, the PE of E. graminis was reduced by 35·4{\%}, suggesting that the inaccessibility was enhanced by the presence of E. pisi. When E. pisi was removed at 1·0 and 3·0 h, the subsequent PEs of E. graminis were 24·8{\%} and 5·8{\%}, respectively, indicating that the prolonged presence of E. pisi further enhanced the inaccessibility. The degree of enhanced inaccessibility was influenced by the size of coleoptile cells but not by the distances between penetration sites of the two fungi within the same cell and the time intervals of the initiation of cytoplasmic aggregation induced by both fungi. It is suggested that unknown mechanism(s) unrelated to papilla formation may be involved in the enhancement of inaccessibility.",
author = "Hitoshi Kunoh and Kazuhiro Toyoda and Naoto Yamaoka and Issei Kobayashi",
year = "1989",
doi = "10.1016/0885-5765(89)90092-1",
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pages = "507--518",
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T1 - Induced accessibility and enhanced inaccessibility at the cellular level in barley coleoptiles. V. Duration of stimulus by a non-pathogen in relation to enhanced inaccessibility

AU - Kunoh, Hitoshi

AU - Toyoda, Kazuhiro

AU - Yamaoka, Naoto

AU - Kobayashi, Issei

PY - 1989

Y1 - 1989

N2 - The influence of the duration of a direct stimulus by a non-pathogen to the enhancement of inaccessibility was examined using barley coleoptile cells and Erysiphe pisi. The degree of enhanced inaccessibility was evaluated with the rate of haustorium formation (penetration efficiency, PE) of E. graminis which attempted penetration of the same coleoptile cells as E. pisi. Partially dissected coleoptiles (coleoptiles A) were inoculated with E. pisi conidia. By observing the specimens every 15 min, the time of initiation of cytoplasmic aggregation in coleoptile cells below an individual E. pisi appressorium was recorded. At 0·5, 1·0 or 3·0 h after the initiation of cytoplasmic aggregation, germlings of E. pisi were removed by a micromanipulator. Germinating conidia of E. graminis which had been inoculated onto other coleoptiles were then transferred onto coleoptile A by micromanipulation so that they could attempt to penetrate the same cells in which E. pisi had induced a cytoplasmic aggregate. When E. pisi was removed 0·5 h after the initiation of cytoplasmic aggregation, the PE of E. graminis was reduced by 35·4%, suggesting that the inaccessibility was enhanced by the presence of E. pisi. When E. pisi was removed at 1·0 and 3·0 h, the subsequent PEs of E. graminis were 24·8% and 5·8%, respectively, indicating that the prolonged presence of E. pisi further enhanced the inaccessibility. The degree of enhanced inaccessibility was influenced by the size of coleoptile cells but not by the distances between penetration sites of the two fungi within the same cell and the time intervals of the initiation of cytoplasmic aggregation induced by both fungi. It is suggested that unknown mechanism(s) unrelated to papilla formation may be involved in the enhancement of inaccessibility.

AB - The influence of the duration of a direct stimulus by a non-pathogen to the enhancement of inaccessibility was examined using barley coleoptile cells and Erysiphe pisi. The degree of enhanced inaccessibility was evaluated with the rate of haustorium formation (penetration efficiency, PE) of E. graminis which attempted penetration of the same coleoptile cells as E. pisi. Partially dissected coleoptiles (coleoptiles A) were inoculated with E. pisi conidia. By observing the specimens every 15 min, the time of initiation of cytoplasmic aggregation in coleoptile cells below an individual E. pisi appressorium was recorded. At 0·5, 1·0 or 3·0 h after the initiation of cytoplasmic aggregation, germlings of E. pisi were removed by a micromanipulator. Germinating conidia of E. graminis which had been inoculated onto other coleoptiles were then transferred onto coleoptile A by micromanipulation so that they could attempt to penetrate the same cells in which E. pisi had induced a cytoplasmic aggregate. When E. pisi was removed 0·5 h after the initiation of cytoplasmic aggregation, the PE of E. graminis was reduced by 35·4%, suggesting that the inaccessibility was enhanced by the presence of E. pisi. When E. pisi was removed at 1·0 and 3·0 h, the subsequent PEs of E. graminis were 24·8% and 5·8%, respectively, indicating that the prolonged presence of E. pisi further enhanced the inaccessibility. The degree of enhanced inaccessibility was influenced by the size of coleoptile cells but not by the distances between penetration sites of the two fungi within the same cell and the time intervals of the initiation of cytoplasmic aggregation induced by both fungi. It is suggested that unknown mechanism(s) unrelated to papilla formation may be involved in the enhancement of inaccessibility.

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