Abstract
Indirect fluorescence detection in cyclodextrin (CD)-modified micellar electrokinetic chromatography (MEKC) was demonstrated by using a diode laser as an excitation source. Amino-substituted polycyclic aromatic hydrocarbons (PAHs), which have no absorption band in the deep-red region, were used as model compounds since it was difficult to separate them by conventional MEKC. In the preliminary study, the separation of amino-substituted PAHs was achieved using γ-CD as a modifier. The fluorescence intensity of the visualizing reagent, oxazine 750, increased in the presence of γ-CD due to the complex formation of oxazine 750 with γ-CD. Consequently, the peak areas of amino-substituted PAHs were observed to decrease with increasing concentration of γ-CD. Detection limits were improved due to the enhancement in efficiency and dynamic reserve by adding small amounts of γ-CD. The results obtained in this study were in good agreement with the proposed model.
| Original language | English |
|---|---|
| Pages (from-to) | 285-292 |
| Number of pages | 8 |
| Journal | Journal of Chromatography A |
| Volume | 831 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 29 1999 |
| Externally published | Yes |
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Keywords
- Detection
- Electrophoresis
- Oxazine 750
- Polycyclic aromatic hydrocarbons
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Indirect detection of amino-substituted polycyclic aromatic hydrocarbons in cyclodextrin-modified micellar electrokinetic chromatography combined with diode laser-induced fluorometry. / Kaneta, Takashi; Saito, Yumiko; Imasaka, Totaro.
In: Journal of Chromatography A, Vol. 831, No. 2, 29.01.1999, p. 285-292.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Indirect detection of amino-substituted polycyclic aromatic hydrocarbons in cyclodextrin-modified micellar electrokinetic chromatography combined with diode laser-induced fluorometry
AU - Kaneta, Takashi
AU - Saito, Yumiko
AU - Imasaka, Totaro
PY - 1999/1/29
Y1 - 1999/1/29
N2 - Indirect fluorescence detection in cyclodextrin (CD)-modified micellar electrokinetic chromatography (MEKC) was demonstrated by using a diode laser as an excitation source. Amino-substituted polycyclic aromatic hydrocarbons (PAHs), which have no absorption band in the deep-red region, were used as model compounds since it was difficult to separate them by conventional MEKC. In the preliminary study, the separation of amino-substituted PAHs was achieved using γ-CD as a modifier. The fluorescence intensity of the visualizing reagent, oxazine 750, increased in the presence of γ-CD due to the complex formation of oxazine 750 with γ-CD. Consequently, the peak areas of amino-substituted PAHs were observed to decrease with increasing concentration of γ-CD. Detection limits were improved due to the enhancement in efficiency and dynamic reserve by adding small amounts of γ-CD. The results obtained in this study were in good agreement with the proposed model.
AB - Indirect fluorescence detection in cyclodextrin (CD)-modified micellar electrokinetic chromatography (MEKC) was demonstrated by using a diode laser as an excitation source. Amino-substituted polycyclic aromatic hydrocarbons (PAHs), which have no absorption band in the deep-red region, were used as model compounds since it was difficult to separate them by conventional MEKC. In the preliminary study, the separation of amino-substituted PAHs was achieved using γ-CD as a modifier. The fluorescence intensity of the visualizing reagent, oxazine 750, increased in the presence of γ-CD due to the complex formation of oxazine 750 with γ-CD. Consequently, the peak areas of amino-substituted PAHs were observed to decrease with increasing concentration of γ-CD. Detection limits were improved due to the enhancement in efficiency and dynamic reserve by adding small amounts of γ-CD. The results obtained in this study were in good agreement with the proposed model.
KW - Detection
KW - Electrophoresis
KW - Oxazine 750
KW - Polycyclic aromatic hydrocarbons
UR - http://www.scopus.com/inward/record.url?scp=0033043372&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033043372&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(98)00925-X
DO - 10.1016/S0021-9673(98)00925-X
M3 - Article
AN - SCOPUS:0033043372
VL - 831
SP - 285
EP - 292
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 2
ER -