Indirect detection of amino-substituted polycyclic aromatic hydrocarbons in cyclodextrin-modified micellar electrokinetic chromatography combined with diode laser-induced fluorometry

Takashi Kaneta, Yumiko Saito, Totaro Imasaka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Indirect fluorescence detection in cyclodextrin (CD)-modified micellar electrokinetic chromatography (MEKC) was demonstrated by using a diode laser as an excitation source. Amino-substituted polycyclic aromatic hydrocarbons (PAHs), which have no absorption band in the deep-red region, were used as model compounds since it was difficult to separate them by conventional MEKC. In the preliminary study, the separation of amino-substituted PAHs was achieved using γ-CD as a modifier. The fluorescence intensity of the visualizing reagent, oxazine 750, increased in the presence of γ-CD due to the complex formation of oxazine 750 with γ-CD. Consequently, the peak areas of amino-substituted PAHs were observed to decrease with increasing concentration of γ-CD. Detection limits were improved due to the enhancement in efficiency and dynamic reserve by adding small amounts of γ-CD. The results obtained in this study were in good agreement with the proposed model.

Original languageEnglish
Pages (from-to)285-292
Number of pages8
JournalJournal of Chromatography A
Volume831
Issue number2
DOIs
Publication statusPublished - Jan 29 1999
Externally publishedYes

Keywords

  • Detection
  • Electrophoresis
  • Oxazine 750
  • Polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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