Indirect detection of amino-substituted polycyclic aromatic hydrocarbons in cyclodextrin-modified micellar electrokinetic chromatography combined with diode laser-induced fluorometry

Takashi Kaneta, Yumiko Saito, Totaro Imasaka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Indirect fluorescence detection in cyclodextrin (CD)-modified micellar electrokinetic chromatography (MEKC) was demonstrated by using a diode laser as an excitation source. Amino-substituted polycyclic aromatic hydrocarbons (PAHs), which have no absorption band in the deep-red region, were used as model compounds since it was difficult to separate them by conventional MEKC. In the preliminary study, the separation of amino-substituted PAHs was achieved using γ-CD as a modifier. The fluorescence intensity of the visualizing reagent, oxazine 750, increased in the presence of γ-CD due to the complex formation of oxazine 750 with γ-CD. Consequently, the peak areas of amino-substituted PAHs were observed to decrease with increasing concentration of γ-CD. Detection limits were improved due to the enhancement in efficiency and dynamic reserve by adding small amounts of γ-CD. The results obtained in this study were in good agreement with the proposed model.

Original languageEnglish
Pages (from-to)285-292
Number of pages8
JournalJournal of Chromatography A
Volume831
Issue number2
DOIs
Publication statusPublished - Jan 29 1999
Externally publishedYes

Fingerprint

Fluorometry
Semiconductor Lasers
Polycyclic Aromatic Hydrocarbons
Cyclodextrins
Chromatography
Semiconductor lasers
Fluorescence
Limit of Detection
Absorption spectra

Keywords

  • Detection
  • Electrophoresis
  • Oxazine 750
  • Polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

@article{7d50f888b5b9462fa01f2f05d82589b8,
title = "Indirect detection of amino-substituted polycyclic aromatic hydrocarbons in cyclodextrin-modified micellar electrokinetic chromatography combined with diode laser-induced fluorometry",
abstract = "Indirect fluorescence detection in cyclodextrin (CD)-modified micellar electrokinetic chromatography (MEKC) was demonstrated by using a diode laser as an excitation source. Amino-substituted polycyclic aromatic hydrocarbons (PAHs), which have no absorption band in the deep-red region, were used as model compounds since it was difficult to separate them by conventional MEKC. In the preliminary study, the separation of amino-substituted PAHs was achieved using γ-CD as a modifier. The fluorescence intensity of the visualizing reagent, oxazine 750, increased in the presence of γ-CD due to the complex formation of oxazine 750 with γ-CD. Consequently, the peak areas of amino-substituted PAHs were observed to decrease with increasing concentration of γ-CD. Detection limits were improved due to the enhancement in efficiency and dynamic reserve by adding small amounts of γ-CD. The results obtained in this study were in good agreement with the proposed model.",
keywords = "Detection, Electrophoresis, Oxazine 750, Polycyclic aromatic hydrocarbons",
author = "Takashi Kaneta and Yumiko Saito and Totaro Imasaka",
year = "1999",
month = "1",
day = "29",
doi = "10.1016/S0021-9673(98)00925-X",
language = "English",
volume = "831",
pages = "285--292",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Indirect detection of amino-substituted polycyclic aromatic hydrocarbons in cyclodextrin-modified micellar electrokinetic chromatography combined with diode laser-induced fluorometry

AU - Kaneta, Takashi

AU - Saito, Yumiko

AU - Imasaka, Totaro

PY - 1999/1/29

Y1 - 1999/1/29

N2 - Indirect fluorescence detection in cyclodextrin (CD)-modified micellar electrokinetic chromatography (MEKC) was demonstrated by using a diode laser as an excitation source. Amino-substituted polycyclic aromatic hydrocarbons (PAHs), which have no absorption band in the deep-red region, were used as model compounds since it was difficult to separate them by conventional MEKC. In the preliminary study, the separation of amino-substituted PAHs was achieved using γ-CD as a modifier. The fluorescence intensity of the visualizing reagent, oxazine 750, increased in the presence of γ-CD due to the complex formation of oxazine 750 with γ-CD. Consequently, the peak areas of amino-substituted PAHs were observed to decrease with increasing concentration of γ-CD. Detection limits were improved due to the enhancement in efficiency and dynamic reserve by adding small amounts of γ-CD. The results obtained in this study were in good agreement with the proposed model.

AB - Indirect fluorescence detection in cyclodextrin (CD)-modified micellar electrokinetic chromatography (MEKC) was demonstrated by using a diode laser as an excitation source. Amino-substituted polycyclic aromatic hydrocarbons (PAHs), which have no absorption band in the deep-red region, were used as model compounds since it was difficult to separate them by conventional MEKC. In the preliminary study, the separation of amino-substituted PAHs was achieved using γ-CD as a modifier. The fluorescence intensity of the visualizing reagent, oxazine 750, increased in the presence of γ-CD due to the complex formation of oxazine 750 with γ-CD. Consequently, the peak areas of amino-substituted PAHs were observed to decrease with increasing concentration of γ-CD. Detection limits were improved due to the enhancement in efficiency and dynamic reserve by adding small amounts of γ-CD. The results obtained in this study were in good agreement with the proposed model.

KW - Detection

KW - Electrophoresis

KW - Oxazine 750

KW - Polycyclic aromatic hydrocarbons

UR - http://www.scopus.com/inward/record.url?scp=0033043372&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033043372&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(98)00925-X

DO - 10.1016/S0021-9673(98)00925-X

M3 - Article

AN - SCOPUS:0033043372

VL - 831

SP - 285

EP - 292

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 2

ER -