Indirect detection by semiconductor laser-induced fluorometry electrokinetic chromatography

Takashi Kaneta, Totaro Imasaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Indirect fluorescence detection of electrically neutral compounds separated by micellar electrokinetic chromatography is performed using a semiconductor laser as an exciting light source. Oxazine 750 is used as a visualizing agent of which absorption maximum is near 680 nm. A surfactant, tetradecyltrimethylammonium chloride, is used to form micelles and to prevent adsorption of oxazine 750 with a positive charge on the capillary wall negatively charged. This surfactant coats on the capillary wall so that oxazine 750 is repulsed electrically on the capillary wall. In this technique, some aromatic compounds with relatively polar functional groups, such as aniline and nitrobenzene, could be separated and detected, while nonpolar compounds such as benzene and toluene can not be detected. The range of the detection limit is from 4.2 × 10-4 to 1.6 × 10-3 M (S/N = 3) for the aromatic compounds. The detection mechanism is based on enhancement of the fluorescence intensity in the micellar solution and on exclusion of the fluorophore attached at the hydrophilic moiety of the micelle by a hydrophilic sample.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages67-70
Number of pages4
Volume2388
ISBN (Print)0819417351
Publication statusPublished - 1995
Externally publishedYes
EventAdvances in Fluorescence Sensing Technology II - San Jose, CA, USA
Duration: Feb 6 1995Feb 8 1995

Other

OtherAdvances in Fluorescence Sensing Technology II
CitySan Jose, CA, USA
Period2/6/952/8/95

Fingerprint

Aromatic compounds
electrokinetics
chromatography
Chromatography
Micelles
Semiconductor lasers
Surface active agents
aromatic compounds
Fluorescence
semiconductor lasers
Nitrobenzene
Fluorophores
micelles
Aniline
surfactants
Functional groups
Toluene
Light sources
Benzene
fluorescence

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Kaneta, T., & Imasaka, T. (1995). Indirect detection by semiconductor laser-induced fluorometry electrokinetic chromatography. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2388, pp. 67-70). Society of Photo-Optical Instrumentation Engineers.

Indirect detection by semiconductor laser-induced fluorometry electrokinetic chromatography. / Kaneta, Takashi; Imasaka, Totaro.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2388 Society of Photo-Optical Instrumentation Engineers, 1995. p. 67-70.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kaneta, T & Imasaka, T 1995, Indirect detection by semiconductor laser-induced fluorometry electrokinetic chromatography. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 2388, Society of Photo-Optical Instrumentation Engineers, pp. 67-70, Advances in Fluorescence Sensing Technology II, San Jose, CA, USA, 2/6/95.
Kaneta T, Imasaka T. Indirect detection by semiconductor laser-induced fluorometry electrokinetic chromatography. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2388. Society of Photo-Optical Instrumentation Engineers. 1995. p. 67-70
Kaneta, Takashi ; Imasaka, Totaro. / Indirect detection by semiconductor laser-induced fluorometry electrokinetic chromatography. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2388 Society of Photo-Optical Instrumentation Engineers, 1995. pp. 67-70
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